[Immunologic study in bone marrow transplantation. Results of the analysis of 3 cases].
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Biomedical subjects
Publications and source records attributed to M Kout.
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These siblings of a Czech family aged 21, 19 and 6 years, respectively, with congenital dyserythropoietic anemia, type II, (HEMPAS) are reported. In two elder siblings ferrokinetic studies revealed a rapid plasma 59Fe clearance, markedly decreased erythrocyte incorporation and shortened 51Cr red-cell survival. Direct anti-globulin test was found positive in one of them. Further investigations revealed low values of blood plasma cholesterol, total lipids, beta-lipoproteins, beta-carotine and vitamin E and A as well as low values of the prothrombin complex. Liver biopsy demonstrated siderosis and disseminated intravascular coagulation in the liver in both patients. The possible reasons for these humoral aberrations are discussed.
In the serum of Wistar rats following intraperitoneal application of methylcellulose (Kabi) the presence of the aggregation factor was confirmed. Using the more sensitive immunohaematological method-enzyme tests-we proved the agglutination phenomenon, which can be of antibody nature and cannot be induced under the test conditions in vitro by action of methylcellulose on blood cells and serum of normal rats. The described phenomenon will only occur if methylcellulose "passes" the organism of the experimental animal.
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A family of gypsy origin, with 5 Fy(a-b-) members was found in Czechoslovakia. The involvement of the silen allele, Fy, in paternity testing is discussed.
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The author evaluates the results of control blood group estimations in the sense of supreme expertise in paternity cases as performed during 7966 through 1974. As ensues from the analysis, the percentage of unconfirmed eliminating conclusions remains to be high, i. e. 5,5%. Different findings as occurring in various group systems have been encountered in 284 cases. Therefore revision of eliminating couclusions cannot be abandoned.