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M Kraml

Publications and source records attributed to M Kraml.

At least 37 records · Page 2Linked to original sources
PubMed1978

An automated method for the analysis of nicotinic acid in serum.

Rational antihyperlipemic therapy based on the use of nicotinic acid and its derivatives and/or combinations demands a rapid and reliable method for monitoring nicotinic acid blood levels. To this end, an automated colorimetric method for the analysis of nicotinic acid in serum has been elaborated. For serum from rats, dogs and man given p.o. nicotinic acid or 3-pyridine methanol, the method is greater than 90% specific for nicotinic acid. The limit detection for nicotinic acid is 2 microgram/ml based on 0.15 ml of serum or 0.3 microgram/ml based on 1.5 ml of serum.

Animals↗
PubMed1977

A gas-liquid chromatographic method for the determination of butriptyline in serum.

1. A gas-liquid chromatographic (GC) method for the analysis of butriptyline in serum has been development. Quantitation is based on the peak height ratio between butriptyline and promazine used as internal standard. A triple partition provides a "clean" extract. A detection limit of 10 ng/ml is achieved. 2. The usefulness of the method has been demonstrated in bioavailability studies in dogs.

Animals↗
PubMed1977

Prodolic acid: analysis and disposition studies in rats and dogs.

1. A specific fluorimetric method for the determination of prodolic acid in serum has been elaborated and has a limit of detection of 2 microgram/ml. 2. Common anti-inflammatory drugs do not interfere with the method. 3. In rats and dogs, prodolic acid is rapidly absorbed and has a short half-life of elimination. 4. The rate of elimination was marked by secondary rises in the blood level profile which were shown to be due enterohepatic recirculation of prodolic acid. 5. Prodolic acid is highly bound to serum protein.

Animals↗
PubMed1977

Effect of ring substitution on the metabolic fate and anti-inflammatory activity of some prodolic acid analogs.

Prodolic acid, 1-n-propyl-1,3,4,9-tetrahydropyrano[3,4-b]indole-1-acetic acid, exhibits potent anti-inflammatory activity in adjuvant arthritic rats. The potency of prodolic acid is enhanced by indole ring substitution. This increase correlated well with higher and sustained drug concentrations in the serum of normal animals. Pharmacokinetic studies demonstrated that ring substitution prolonged the serum half-life without affecting the absorption or volume of distribution. Because, in the rat, indole ring hydroxylation is a major pathway for the disposition of prodolic acid, we ascribe the increased pharmacological activity of ring substituted derivatives to the interference of substituents with the hydroxylation reaction.

Animals↗
PubMed1975

A gas-liquid chromatographic determination of p-chlorophenoxyisobutyric acid and its comparison to an ultra violet method.

1. A gas-liquid chromatographic procedure for the determination of p-chlorophenoxyisobutyric acid (CPIB) has been elaborated and compared to the UV spectrophotometric procedure of Barrett and Thorp. 2. Both methods are specific when used to determine CPIB levels in normal sera from laboratory animals or humans treated with clofibrate (Atromid-S). Serum from patients treated with other drugs or abnormal sera from patients affected with a variety of diseases will often contain high and fluctuating levels of non-specific UV absorbing substances and this usually precludes the use of the UV procedure. 3. The GLC method is also applicable to the analysis of CPIB in urine samples.

Animals↗