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M Kriz

Publications and source records attributed to M Kriz.

14 recordsLinked to original sources
PubMed1977

[Prolactin].

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Female↗
PubMed1977

[Epidural analgesia--effect on the fetus and uterine activity].

The effect of continuous epidural analgesia on the fetus, newborn, and uterine activity was studied in a group of 50 parturients. No untoward effects, either in the fetus or uterine activity, that could be ascribed to this procedure, were observed. The authors use their own modicifation of the epidural block by titrating the amount of the local anesthetic according to the intensity of pain in labour.

Anesthesia, Epidural↗
PubMed1976

Stimulation of prolactin synthesis and of adenosine 3':5'-cyclic phosphate formation by prostaglandins and thyroliberin in cultured rat pituitary cells.

1. The effects of prostaglandins E2 and F2alpha on prolactin synthesis were examined in a clonal strain of rat pituitary tumour cells, and compared with those of thyroliberin. 2. The prostaglandins and thyroliberin gave a dose-related and time-dependent stimulation of prolactin synthesis. The maximal effects (about twofold increases) were observed after 54h of treatment with 25nM-prostaglandin E2 and 2.5nM-prostaglandin F2alpha. A similar stimulation of prolactin synthesis was observed after 250nM-thyroliberin. The combined treatment with prostaglandins and thyroliberin did not increase prolactin synthesis over and above that obtained with each compound alone. 3. After removal of prostaglandins E2 and F2alpha there was a complete reversal of prolactin synthesis to pre-stimulation values 18h later (t1/2less than or equal to 9h). The rapid reversible effect of prostaglandins was in contrast with that of thyroliberin, where prolactin synthesis returned to control values with a t1/2 of about 42 h. 4. Prostaglandin E2 (5mum) and thyroliberin (5mum) increased cellular concentrations of cyclic AMP eight- and four-fold respectively. Maximal effects were observed after 2-5min of incubation. The increases in cyclic AMP were biphasic; normal values were obtained 60 min after the start of incubation with prostaglandin E2 or thyroliberin. 5. The dose/response curve showed that prostaglandin E2 caused maximal increase of cyclic AMP at 50nM. Concentrations of prostagland in E2 that caused half-maximal stimulation of cyclic AMP accumulation and of prolactin synthesis were 4 and 5nM respectively. 6. Combined treatment with prostaglandin E2 and thyroliberin in concentrations that separately caused maximal cyclic AMP increases did not result in a further increase in this cyclic nucleotide. 7. These results are consistent with a role of cyclic AMP in mediating the effects or prostaglandins and thyroliberin on prolactin synthesis. However, if cyclic AMP is involved as a common intracellular mediator of prolactin synthesis, it cannot alone explain all the effects of prostaglandins and thyroliberin in this cell system.

Animals↗
PubMed1976

Measurements of prolactin and growth hormone synthesis and secretion by rat pituitary cells in culture.

A specific and sensitive immunoprecipitation method for measurements of biosynthesized radioactive prolactin and growth hormone is described. Antisera to rat prolactin and growth hormone were developed in the rabbit and monkey, respectively. The specificity of the immune sera was assessed by polyacylamide gel electrophoresis of the dissolved immunoprecipitates. The two antisera showed cross-reactions with the nonhomologous hormone of less than 1%. Separation of tritium-labelled prolactin and growth hormone by immunoprecipitation, followed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate was shown to be 95-57% complete. When both hormones were measured in the same microsample by sequential immunoprecipitation, the reaction was 97% complete for determination of intra- and extracellular prolactin and extracellular growth hormone, but 85% complete for determination of intracellular growth hormone. This method has been used to characterize the basal synthesis and secretion of prolactin and growth hormone in three different but related, pituitary cell strains. Radioactive prolactin and growth hormone was obtained from monolayer cultures when the cells were grown in the presence of [3H]L-leucine. The rate of prolactin synthesis and extracellular accumulation was higher than that of growth hormone in a cell strain which produced both hormones. In these cells prolactin synthesis represents 1-5%, and growth hormone 0.1-0.6% of total protein synthesis.

Animals↗
PubMed1976

Effects of prostaglandins on prolactin and growth hormone synthesis and secretion in cultured rat pituitary cells.

The effects of prostaglandins E1, E2, F1alpha, and F2alpha on prolactin and growth hormone synthesis and secretion were studied in cultured rat pituitary cells. Total extracellular accumulation of prolactin was measured by radioimmunoassay. When hormone synthesis was studied, the cells were cultured in the presence of [3H]leucine for the last hour of each treatment period. The intra- and extracellular radioactive hormones were determined in the same microsample by a specific and quantitative immunoprecipitation method, employing polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. The results show that all prostaglandins stimulated the extracellular accumulation of prolactin at 3-30 nM. Prostaglandin E1 was more effective in stimulating extracellular accumulation of prolactin than prostaglandin E2 and the F compounds. Prostaglandin F2alpha at 3 nM doubled the rate of prolactin synthesis, but had no effect on growth hormone or total cell protein synthesis after 24 h of treatment. The first effect of prostaglandin F2alpha was observed after 5 h of incubation, and the time-course of effect on prolactin synthesis was similar to that of thyrotropin-releasing hormone.

Animals↗
PubMedDate not supplied

[Epilepsy with centrotemporal (Rolandic) spikes. A peculiar seizure disorder of childhood].

The study presents the results of a long term investigation of 60 epileptic children with Rolandic (centrotemporal) spikes in EEG. The results could be summarized as follows: the Rolandic epilepsy is relatively frequent entity (13.4% of the total number of epileptic children). The age span was from 3--13 years, with a peak of age incidence between the 7th and 8th year of life. More than half of children had nocturnal fits only. From the clinical point of view 60% of children had generalized crises, and the remaining 40% had partial attacks corresponding to the functional organization of the Rolandic cortical area. The evolution of the Rolandic epilepsy in childhood is favourable. More than 50% didn't have more attacks after the introduction of antiepileptic therapy, and 3/4 of them could be classified as practically cured after an long-term follow-up (criterion: an attack-free period of at least 5 years. Finally, in more than 80% of cases after three years of follow-up the spikes have disappeared from the EEG tracings which were completely normal.

Adolescent↗