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Biomedical subjects

M Krutsay

Publications and source records attributed to M Krutsay.

At least 19 recordsLinked to original sources
PubMed1990

[The role of permeability in histological staining].

Ionic stain-binding is influenced by number of acid and basic groups, their dissociation equilibrium coefficient and isoelectric point of proteins, respectively, and by permeability, on tissue side and acid and basic character, respectively, ionic volume, isochronic and succedaneous way of application, respectively, and hydrogene ion concentration of solution on side of stain. With joint or sequent use of acid stains of different ionic volume, permeability order of certain tissue elements, their relative permeability can be determined.

Histological Techniques↗
PubMed1989

[The impregnation of glial fibers].

The author studied the influence of the solvents and of the rinsing fluids on the result of silver impregnation. A titrimetric method is described for the determination of the concentration of formaldehyde solutions.

Formaldehyde↗
PubMed1989

[Differential staining of calcium compounds in histologic sections].

An experimental protocol is proposed for the differentiation of calcium-hydrogen-phosphate, tri-calcium-phosphate, calcium-oxalate, calcium-urate and fatty acidic calcium. For tissue fixation calcium acetate-formaldehyde is recommended in which fatty acids and earth-metal urates form insoluble calcium-salts which can be demonstrated in paraffin sections.

Animals↗
PubMed1989

[Fixation of tissue specimens. II].

As a routine fixative calcium-acetate-formol is recommended causing no tissue shrinkage, haemolysis, formaldehyde pigments and having an almost neutral pH. Due to the formation of insoluble calcium salts it preserves fatty acids and water-soluble urates and makes them withstanding to paraffin embedding. If completed with methylene blue this solution is also suitable for an improved fixation of acidic glycosaminoglycans.

Acetates↗
PubMed1988

[Amyloid staining. III].

Anisotrophy of collagen fibres can be abolished, without change of the anomalous colour of amyloid, by mounting Congo-red stained sections in glycerin. Treatment with sodium hydroxide solution prevents staining of secondary amyloid with Sirius red.

Amyloid↗
PubMed1987

[A new argyrophil reaction].

A method is described, which is based on mordantiation with a concentrated silver nitrate solution at 60 degrees C followed by two-step physical development at room temperature, with formalin as the reducing agent.

Animals↗
PubMed1987

[The staining of amyloid].

All types of amyloid can be quickly and selectively demonstrated by staining with an alcohol-containing Sirius red solution.

Amyloid↗
PubMed1986

[The histochemical use of alcian green].

With the hydrochloric acid-alcoholic solution of alcian green, without any pretreatment both the sulfated and the carboxylated proteoglycans can be demonstrated. After an indirect sulfation (periodic acid-bisulfite pretreatment), the glycogen and the glycoproteins, and after an oxidation with acidified potassium permanganate, the high cystine-containing proteins can also be stained. As a counterstain, a picric acid-Sirius red solution has proved good.

Coloring Agents↗
PubMed1986

[Demonstration of polysaccharides by silver impregnation (2)].

To demonstrate carbohydrate-containing tissue elements becoming argyrophil by treatment with chromic acid and periodic acid respectively, the author proposes instead of the methenamine-silver nitrate solution the successive application of a silver nitrate solution, adjustable to the optimal pH, and of a physical developer.

Humans↗