Biomedical subjects

M Kuffer

Publications and source records attributed to M Kuffer.

6 recordsLinked to original sources

PubMed1998

Karnofsky's score modified for cats.

The index of Karnofsky evaluating quality of life was originally developed by David A. Karnofsky in 1948. It determines the ability of a patient to carry on normal activities in life by using a scale from 0 to 100%. This index was modified for the cat. The index enables judgment of life quality and well-being in cats which is very difficult to interpret. Objectivity is given by a classification orientated in details concerning general condition as well as eating, playing, sleeping, and social behavior.

Animals↗

PubMed1998

Efficacy of the acyclic nucleoside phosphonates (S)-9-(3-fluoro-2-phosphonylmethoxypropyl)adenine (FPMPA) and 9-(2-phosphonylmethoxyethyl)adenine (PMEA) against feline immunodeficiency virus.

The acyclic nucleoside phosphonates (S)-9-(3-fluoro-2-phosphonylmethoxypropyl)adenine (FPMPA) and 9-(2-phosphonylmethoxyethyl)adenine (PMEA) were evaluated for their efficacy and side effects in a double-blind placebo-controlled trial using naturally occurring feline immunodeficiency virus (FIV)-infected cats. This natural retrovirus animal model is considered highly relevant for the pathogenesis and chemotherapy of HIV in humans. Both PMEA and FPMPA proved effective in ameliorating the clinical symptoms of FIV-infected cats, as measured by several clinical parameters including the incidence and severity of stomatitis, Karnofsky's score, immunologic parameters such as relative and absolute CD4+ lymphocyte counts, and virologic parameters including proviral DNA levels in peripheral blood mononuclear cells (PBMC) of drug-treated animals. In contrast with PMEA, FPMPA showed no hematologic side effects at a dose that was 2.5-fold higher than PMEA.

Adenine↗

PubMed1997

[Comparative investigation of the efficacy of two nucleocapsid analogs in FIV infected cats].

Antiviral activity of the acyclic nucleoside phosphonates 9-(2-phosphonylmethoxyethyl)adenine (PMEA) and 9-(3-fluoro-2-phosphonylmethoxypropyl)adenine (FPMPA) against feline immunodeficiency virus (FIV) was investigated in field cats. The study was designed as a placebo-controlled double-blind study enrolling 27 FIV infected cats. Nine cats received PMEA at a dosage of 10 mg/kg body weight, nine cats received FPMPA at a dosage of 25 mg/kg body weight. A variety of parameters were established to evaluate the therapeutic efficacy of the compounds. The improvement was monitored by different aspects: clinical status, index of Karnofsky modified for the cat, laboratory parameters, immunological parameters, surrogate markers, and a virological parameter. Concerning the clinical and immunological parameters cats of both treatment groups disclosed a relevant improvement. Efficacy of antiviral treatment was a little bit higher in the cats treated with PMEA than in the animals injected with FPMPA. However, side effects were also more prominent in the PMEA treated group.

Adenine↗

PubMed1995

[Rapid enzymatic test for diagnosis of parvovirus infections in dogs].

In this study 52 canine fecal samples were examined for the presence of canine parvovirus (CPV). The two different test systems used to confirm infection were electron microscopy as standard method and an enzyme-linked immunosorbent assay (ELISA). In comparison, the results conferred in 92.3%. The CPV ELISA had a sensitivity of 89.7% and a specificity of 100%. Therefore, it can be used as a quick, reliable method for diagnosis of canine parvovirus infection in veterinary practice.

Animals↗

PubMed1995

[Treatment of severe neutropenias in dogs and cats with filgrastim].

The recombinant hG-CSF Filgrastim was used in severe cases of neutropenia in the dog caused by parvovirosis, hyperestrogenism, treatment with antineoplastic agents, an aplastic syndrome and in the cat in cases of infectious panleukopenia. Increases of the numbers of leukocytes were observed in all groups of diseases in the dog, but not in feline infectious panleukopenia. Filgrastim is indicated in neutropenias associated with disturbance of the general condition accompanied by fever, but not in cases of transitory leukopenias.

Anemia, Aplastic↗

PubMed1994

[Diagnosis of FIV infection].

The FIV PetCheck ELISA is a practical and commercially available method routinely used for the detection of anti-FIV antibodies in FIV infection. However, false positive and false negative results occur, although the reliability of the negative results is higher than that of the positive results. To determine the predictive value of a positive result in Bavaria, 1911 randomly selected cat sera were collected. Using the PetCheck anti-FIV ELISA, 103 (5.4%) of the 1911 samples tested were positive. These positive samples were reexamined by two other ELISA and by Western Blot. Sixty-one (59.2%) were positive with the second ELISA, 53 (51.5%) with the third ELISA and 40 (40.7%) by Western Blot. Therefore, 59.2% of all positive samples could be considered false positive. The ELISA as a single method to diagnose FIV infection is not reliable in Germany due to the low prevalence of FIV infection. Therefore, a Western Blot confirmation of every positive ELISA is of absolute necessity.

Animals↗