Biomedical subjects
M Kuligowski
Publications and source records attributed to M Kuligowski.
Ultrastructural changes of the skin induced by human leukocyte elastase and cathepsin G.
Human neutrophil elastase and cathepsin G at a concentration of 10(-6) M were found to attack various substrates when normal skin biopsy specimens were incubated at 37 degrees C for 1 h with either of these enzymes. Elastase damaged primarily hemidesmosomes, leading to the epidermal cleavage from the dermis, whereas cathepsin G damaged the membrane structures. Both these neutral proteinases were highly specific to basal lamina of blood vessels. This indicates that neutrophil elastase and neutrophil cathepsin G may play different roles in various skin diseases related to enhanced activity and infiltration of neutrophils.
Basement membrane zone as a target for human neutrophil elastase in psoriasis.
Human neutrophil elastase was found, by indirect immunofluorescence using rabbit anti-elastase anti-serum, to be bound to basement membrane of psoriatic plaques in vivo. The enzyme was also identified inside the migrating neutrophils in the reticular dermis and dermal papillae, as well as outside the cells in micro-abscesses in psoriatic skin. In vitro incubation of normal skin with human neutrophil elastase resulted in the destruction of hemidesmosomes and separation of the epidermis from the dermis above localizations of bullous pemphigoid antigen. These findings are direct evidence that human neutrophil elastase could play a role in psoriasis in in vivo destruction of the epidermal-dermal junction.
Apoptosis in Bowen's disease. An ultrastructural study.
We performed ultrastructural studies of apoptosis (previously referred to as "malignant dyskeratosis") in a case of genital Bowen's carcinoma in which human papillomavirus (HPV) type 33 genome was identified and in two cases of cutaneous Bowen's disease with no detectable viral DNA; herein we present the sequential stages in the development of apoptotic bodies. The apoptotic process in the HPV-containing genital Bowen's disease was similar to that in the cutaneous lesions with no detectable HPV. The presence of a large number of apoptotic bodies in Bowen's disease may be responsible for the slow progression and noninvasive growth of this carcinoma in situ.
[Cutaneous mastocytosis with bullous changes simulating xanthoma tuberosum].
A 20-year-old female patient is reported with urticaria pigmentosa and coexisting nodules (mastocytoma) resembling clinically lesions of the type of xanthoma tuberosum. An analysis of microscopic image and ultrastructural changes was carried out and modern views on treatment of cutaneous mastocytosis are discussed.
[Pagetoid reticulosis (Woringer-Kolopp disease)].
The first Polish report of a case of the limited variety of the Woringer-Kolopp disease (pagetoid reticulosis) is presented. The diagnosis was based on histological examination. Numerous pagetoid cells were found in the epidermis and only few in the dermis. In ultramicroscopy two types of mononuclear cells were disclosed, one with large nucleus and scattered chromatin, and other with smaller nucleus and nuclear chromatin condensed at the periphery. The immunopathological examination of isolated pagetoid cells with monoclonal antibodies showed on their surface a slight prevalence of suppressor T-cell receptors. Radiotherapy was applied with a very good result.
[Epidermal apoptosis and its significance as a systemic phenomenon].
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[Electron microscopy studies of apoptosis in bowenoid papulosis].
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