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Biomedical subjects

M Kurono

Publications and source records attributed to M Kurono.

69 records · Page 4Linked to original sources
PubMed1982

Isolation of recombinants doubly and triply drug-resistant to streptomycin, tetracycline and chloramphenicol by PEG-induced cell fusion of singly resistant staphylococcus aureus L-forms.

Various combinations of four substrains of Staphylococcus aureus L-form (strain STA-EMT-1), each of which was resistant to one of the following four drugs, streptomycin (SM), tetracycline (TC), chloramphenicol (CP) and erythromycin (EM), were submitted to polyethylene glycol (PEG)-induced cell fusion. PEG-induced cell fusion followed by enrichment culture in the liquid basal medium supplemented with penicillin G resulted in development of recombinants that were doubly drug-resistant to SM and TC, SM and CP, and TC and CP, but no recombinant doubly resistant to EM and TC, was obtained by treatment of a EM-resistant and TC-resistant substrains with PEG. No recombinants resistant to SM, CP and TC could be obtained by treatment of substrains resistant to SM, CP and TC, respectively, with PEG. But recombinants triply resistant to these three drugs were produced by two-step cell fusion; that is by fusion of a recombinant doubly resistant to two of the three drugs with a substrain resistant to the third drug.

Chloramphenicol↗
PubMed1980

Further evidence of polyethylene glycol-induced cell fusion of Staphylococcus aureus L-forms.

Further evidence is presented that on treatment with polyethylene glycol the emergence of doubly drug-resistant recombinants of Staphylococcus aureus L-forms from substrains resistant to streptomycin or erythromycin only is really due to cell fusion, and not to other genetic transfer mechanisms. The reason for the necessity of enrichment culture is also discussed.

Drug Resistance, Microbial↗
PubMed1976

The levels of main urinary metabolite of prostaglandin F1alpha and F2alpha in human subjects measured by radioimmunoassay.

Radioimmunoassay technique for measuring 5alpha, 7alpha-dihydroxy-11-keto-tetranorprosta-1, 16-dioic acid, the main urinary metabolite of PGF1alpha and PGF2alpha (PGF2alpha-MUM), was further improved. It was postulated based on some experimental data that the PGF2alpha-MUM exists in the urine mostly as dioic acid form, not as delta-lactone form the daily excretion of PGF2alpha-MUM in men ranged from 14.43 mug to 36.14 mug and in women from 5.21 mug to 14.25 mug.

Female↗
PubMedDate not supplied

Effect of long-term treatment with a new aldose reductase inhibitor, (2S,4S)-6-fluoro-2',5'-dioxospiro-[chroman-4,4'-imidazolidine]-2-carbox amide (SNK-860), on peripheral neuropathy in streptozotocin-induced diabetic rats.

We studied the long-term effects of a new aldose reductase inhibitor, (2S,4S)-6-fluoro-2',5'-dioxospiro-[chroman-4,4'-imidazolidine]-2- carboxamide (SNK-860), on functional, biochemical, and structural changes in peripheral nerve of streptozotocin (STZ)-induced diabetic rats. During the experimental period of 26 weeks, the delayed motor-nerve conduction in diabetic rats was significantly prevented by SNK-860 treatment, and elevated sorbitol levels and reduced myo-inositol levels were normalized to 100% and 71% of control levels, respectively. Teased nerve fiber studies demonstrated that the frequency of abnormal fibers was significantly reduced in treated diabetic rats. Morphometric analysis of myelinated fibers also disclosed prevention of axonal atrophy, distorted axonal circularity and preservation of large-sized fibers following SNK-860 treatment. These results suggest that long-term treatment with SNK-860 has a beneficial preventive effect on the development of experimental diabetic neuropathy.

Aldehyde Reductase↗