PubMed Health⌕ Search

Biomedical subjects

M Kyo

Publications and source records attributed to M Kyo.

86 records · Page 5Linked to original sources
PubMed1991

[Differential diagnosis of kidney transplant rejection and ciclosporin nephrotoxicity by urine cytology].

To make the differentiation of kidney transplant acute rejection and ciclosporin (CS) nephrotoxicity urine cytology by classical Papanicolaou with immunocytochemical stain has been performed. Increased numbers of renal tubular cells with lymphocytes and monocytes were found in both rejections and CS toxicities. CS toxicities were associated with increased numbers of proximal tubular cells. In immunocytochemical stain, increased numbers of CD25 and CD8 positive cells as well as increased ratio of HLA-DR/cytokeratin positive cells were typically found in rejections. It is concluded that the proposed analysis of urine cytology is a non-invasive and reliable method for daily graft monitoring of acute rejection and CS toxicity.

CD8 Antigens↗
PubMed1991

[Transurethral balloon dilatation of the prostate: initial results, indication and complication].

Transurethral balloon dilatation therapy was performed on 40 patients with benign prostatic hypertrophy (BPH) under local anesthesia. During the procedure, urinary urgency occurred in 80% of the patients. After prostatic dilatation, macrohematuria was observed in almost all patients. The longest follow-up period after dilatation was now 22 months, and the average was 9.5 months. After treatment, residual urine volume decreased, and average flow rate and maximal flow rate improved from 5.7 and 10.4 ml/sec to 8.2 and 15.6 ml/sec, respectively. Overall clinical efficacy was 67.5%. Urethral dilatation therapy was thought to be an effective and non-invasive therapy for BPH.

Aged↗
PubMed1991

Morphologic findings in "zero-hour" biopsies of renal transplants.

The extreme lack of renal grafts for transplantation stimulated us to analyze how strict the selection criteria of kidney donors must be. We investigated therefore if preexisting lesions in renal grafts influence initial and late renal function. 147 zero-hour biopsies of 101 donors (mean age 33, from 6-64 years) were examined. By ligh microscopy 38% of biopsies showed no, 44% showed nonspecific and 18% specific lesions. Nonspecific lesions comprised intimal fibrosis of small arteries in 44%, interstitial fibrosis in 8% and an arteriolar hyalinosis in 29%. Out of 102 immunohistologically examined biopsies 74.5% showed nonspecific IgM/C3 deposits in glomeruli and/or arterioles. An age dependent decrease of normal renal biopsies was found which was most evident in donors older than 40 years. Specific findings consisted of glomerulosclerosis (n = 4), glomerulonephritis (n = 11), intravascular coagulation (n = 10) and eclamptic kidney (n = 1). In case of nonspecific immunohistologic findings and in glomerulonephritis rebiopsies showed that antigen deposits usually disappeared within 4 months. Independent of morphologic findings 82% of transplant recipients had a good initial and late renal function. Since donor age, glomerulosclerosis, glomerulonephritis, intravascular coagulation or eclamptic changes seem not to compromise renal function after transplantation a more liberal choice of donors should be considered.

Adult↗
PubMed1990

Possible mechanism in rat with surviving heart allograft after short course of 15-deoxyspergualin treatment.

15-Deoxyspergualin has been reported to be a useful immunosuppressive agent, that is already used to inhibit acute rejection, in clinical transplantation. In the present study, the survival of heart allograft in rats after a short course of DSG treatment and the mechanism underlying induction of DSG-induced heart allograft survival, in the early phase, were studied. Male LEW rats were used as recipients. Male ACI and Wistar rats were used as donors and third party donors, respectively. Survival of ACI heart grafts in LEW recipients, treated with a short course of DSG, beginning from day 4 of grafting, were markedly prolonged, with a mean survival time of 16.6 +/- 5.8 days and 29.8 +/- 3.0 days at the dose of 2.5 mg/kg/day and 5 mg/kg/day, respectively. The mechanism of inducing allograft survival in the early phase, after a short course of DSG treatment, was further analyzed by testing the ability of splenic lymphocytes or serum in MLR assays and transfer experiments. The addition of various concentrations of splenic lymphocytes or serum from DSG treated LEW rat with surviving ACI heart allograft to MLR showed a strong suppression in a cell-dose-dependent manner and serum-dose-dependent manner, both in donor strain and third party MLR. An interesting finding was that there was a constant differential suppressive rate between donor strain party MLR and third party MLR. Moreover, transfer of 2.0 x 10(8) splenic lymphocytes or 2 ml serum from DSG-treated LEW rat with surviving ACI heart allograft, to sublethally irradiated grafted host, did not prolong survival both in ACI heart grafts and third party Wistar grafts. These results suggest that the strong suppresser cells and the suppressor humoral factor(s) play, at least, a partial role in the early phase of rats bearing allograft after a short course of DSG treatment.

Animals↗
PubMed1989

[Interstitial and glomerular changes in kidney transplants].

This review gives a survey of the most important tubulo-interstitial and glomerular lesions in kidney transplants. Among the tubulo-interstitial lesions subcapsular interstitial fibrosis, acute tubular necrosis, cellular tubulo-interstitial rejection and Ciclosporin associated lesions are briefly described. The review of the glomerular lesions is focused upon intraglomerular coagulation, transplant glomerulitis and transplant glomerulopathy. Among the immunocomplex diseases the preexisting glomerular lesions are stressed. Furthermore the differential diagnosis between rejection associated glomerular lesions and glomerulonephritis is discussed. Finally the prognostic significance of the different morphologic lesions is outlined based on an analysis of 500 biopsies from Basel.

Antigens, CD↗
PubMed1988

[Transverse ectopia of the testis. A case report].

A case of transverse ectopia of the right testis with left inguinal hernia is reported. A one-year-old-boy was admitted to our hospital with swelling of the left scrotal contents and right undescended testis. At operation, two testes were situated on the left side, one in the left scrotum and another in the left inguinal canal. Right transseptal orchiopexy was performed. Including our case, we discovered 73 cases of transverse ectopia of the testes in the Japanese literature. A brief discussion was made about the disease.

Abdominal Neoplasms↗
PubMed1987

[A case of angiomyolipoma originating from polycystic kidney with horseshoe kidney].

A case of renal angiomyolipoma originating from polycystic kidney with horseshoe kidney is reported. A 32-year-old woman visited our hospital with the complaint of proteinuria. with computerized tomographic scan and further examinations the patient was diagnosed as having renal angiomyolipoma with tuberous sclerosis. The tumor originated from a polycystic horseshoe kidney. Three weeks later, she complained of right flank pain and was diagnosed with spontaneous rupture of the angiomyolipoma. Right heminephrectomy was performed and histological examination confirmed the preoperative diagnosis. Some discussion is made on the characteristics and treatment of renal angiomyolipoma, and the statistics on renal diseases with tuberous sclerosis in Japan are presented.

Adult↗
PubMed1985

Studies on Conditions for Cell Division and Embryogenesis in Isolated Pollen Culture of Nicotiana rustica.

A method for the induction of a high rate of cell division and embryogenesis of Nicotiana rustica pollen was developed. Binucleate pollen grains were fractionated by Percoll density gradient (35/45%) centrifugation and cultured in 0.4 molar mannitol at 30 degrees C (the first culture). After 3 days in culture pollen was recollected by a second Percoll fractionation (0/30%) and transferred to and cultured in a medium containing the Murashige-Skoog macro-elements, 0.4 molar mannitol, 40 millimolar galactose, 3 millimolar glutamine, and 5 micromolar ABA for 10 days (the second culture). The cell population consisting of about 80% dividing pollen was transferred to a Murashige-Skoog medium containing 0.4 molar mannitol, 3 millimolar glutamine, and no phytohormone (the third culture), where about 40% of dividing pollen developed into embryos or embryogenic calli.

Journal Article↗
PubMedDate not supplied

Giant mitochondria in "zero-hour" transplant biopsies.

Giant mitochondria in kidneys have increasingly been observed since the introduction of Cyclosporin A (CSA) as immunosuppressant in kidney transplants and in patients with autoimmune disorders. In animals treated with CSA, giant mitochondria were also described. In a study of "zero-hour" biopsies taken immediately before or after reperfusion of the renal transplant, giant mitochondria were often unexpectedly found. Retrospective analysis revealed that a significant increase in the number of giant mitochondria is more often associated with CSA therapy than ischemia. Giant mitochondria with sparse cristae seem to prevail in ischemia, whereas those with dense matrix and crystalloid structures predominate in CSA therapy.

Adolescent↗