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M L Cibull

Publications and source records attributed to M L Cibull.

44 records · Page 3Linked to original sources
PubMed1982

Evaluation of methods of detecting terminal deoxynucleotidyl transferase in human hematologic malignancies. Comparison of immunofluorescence and enzymatic assays.

Terminal transferase (TdT) activity and antigen have been measured in 267 specimens of human bone marrow and peripheral blood by using a biochemical assay for enzymatic activity and an immunofluorescence test for antigen. Oligo p(dA)50 and dGTP were used as reagents in the biochemical assay and either rabbit anti-calf TdT or rabbit anti-human TdT was used as the primary antibody for immunofluorescence. Because both false-positive and false-negative detection of TdT antigen occurs, the biochemical assay of TdT activity is considered the standard against which immunofluorescence assays must be measured. If specimens of cells contained TdT activity, then the immunofluorescence detected antigen in 91% of cases (rabbit anti-calf TdT) and 95% of cases (rabbit anti-human TdT). When no TdT activity was detected, the immunofluorescence test was positive in 7.8% of cases (rabbit anti-calf TdT) and 5.2% of cases (rabbit anti-human TdT). When air-dried slices were shipped by air mail to a distant location before being stained for immunofluorescence, TdT antigen was detected in only 33% of matched pair cases which contained TdT activity. From this study, the authors conclude that with current methodology, immunofluorescence tests for TdT antigen must be carried out on slides prepared in the testing laboratory and that such tests are reliable in more than 90% of cases. However, because a small percentage of results are false positives and false negatives, the authors suggest that if a patient's clinical response is not consistent with the immunofluorescence TdT result, an enzymatic assay for TdT activity be carried out.

Antigens↗
PubMed1981

Cutaneous malignant lymphoma: a pathologic study of 50 cases with clinical analysis of 37.

Fifty cases of malignant lymphoma, exclusive of mycosis fungoides, with initial involvement of the skin are reported. The head and neck was the most common site of presentation and 44% of the cases were classified as the diffuse histiocytic type. The cases encompassed virtually the entire spectrum of histologic subtypes of non-Hodgkin's lymphomas; these subtypes were a major prognostic factor in predicting the subsequent clinical course. Among 37 patients with available follow-up information, those patients with a "favorable" histology had a significantly better five-year freedom from relapse (70% vs. 19%) and survival (88% vs. 30%) compared with those patients with the "unfavorable" types of lymphoma. clinical staging was another important variable in determining prognosis. Fifteen patients with disease confined to skin had a significantly longer freedom from relapse (44% vs. 23%) at five years than 22 patients in whom staging procedures revealed extracutaneous disseminated lymphoma. Thirteen of the patients with widespread lymphoma have died from 2-30 months after diagnosis (median survival ten months), in contrast with only four deaths among the patients with limited disease. The pattern of relapse for these latter patients tended to involve cutaneous sites distant to the original lesion. This observation may have a bearing on future therapy of cutaneous lymphomas.

Adolescent↗
PubMed1981

Pseudolymphoma of the stomach. A diagnostic and therapeutic dilemma.

Pseudolymphoma is an uncommon benign lesion of the stomach that poses a difficult problem in diagnosis and management. The clinical manifestations and endoscopic, radiologic, and biopsy findings are not generally helpful in making this diagnosis preoperatively. Histologic examination of the lesion is the only reliable method that distinguishes pseudolymphoma from true lymphoma. Distinguishing histologic features of pseudolymphomas are (1) formation of true germinal centers, (2) presence of a polymorphous inflammatory infiltrate, and (3) absence of lymph nodal involvement by lymphoma. We report four cases and review the literature to illustrate the features of pseudolymphoma. Subtotal gastric resection is done for diagnostic as well as for therapeutic purposes. Distinction of these benign lesions from malignant lymphomas is important so that unnecessary radical surgery and postoperative radiation therapy or chemotherapy are avoided.

Adult↗
PubMed1981

Unusual immunofluorescence patterns for terminal deoxynucleotidyl transferase in blast crisis chronic myelogenous leukemia.

An unusual cytoplasmic distribution of terminal deoxynucleotidyl transferase (TdT) antigen in leukemic cells from two patients who had chronic myelogenous leukemia in blastic phase is described. In most leukemic cells that contain TdT, the intracellular location has been reported to be exclusively nuclear. The cells from these two patients demonstrated TdT staining in both the nucleus and the cytoplasm. The pattern is remarkably similar to that observed in thymocytes, in which bright cytoplasmic staining may also be seen. In the immunofluorescence procedures for detection of TdT in blasts from patients who have chronic myelogenous leukemia, significant cytoplasmic staining should not be mistaken for nonspecific absorption of immunoglobulins or specimen deterioration.

Adult↗
PubMed1978

Incidental discovery at radical mastectomy of inapparent Hodgkin's disease in long term survivors.

Two long-term survivors with nodular sclerosing Hodgkin's disease were found to have adenocarcinoma of the breast sixteen and twenty years after limited field radiotherapy. Examination of the axillary nodes at radical mastectomy showed both metastatic adenocarcinoma and clinically inapparent Hodgkin's disease. The occurrence of persistent but clinically inactive Hodgkin's disease as well as the development of secondary malignancies in this disease has important clinical ramifications. Skin testing and in vitro lymphocyte transformation studies in these two patients were also done.

Adenocarcinoma↗
PubMed1978

Ultrastructure of osteoclastoma-like giant cell tumor of thyroid.

An aggressive anaplastic tumor of the thyroid with osteoclastoma-like giant cells was studied by electron microscopy. The lack of junctional complexes or other obvious epithelial elements suggested mesenchymal origin. The giant cells resembled osteoclasts with respect to mitochondria, rough endoplastic reticulum, lysosomes, ribosome complexes and vacuoles, but the prominent nuclear golgi apparatus of giant cell tumors of bone was absent.

Aged↗
PubMedDate not supplied

Bone marrow metastases from small cell cancer of the head and neck.

BACKGROUND: Primary small cell carcinoma of the head and neck is rare. Although the larynx is the most prevalent site of head and neck small cell carcinoma (SCC), this report will concentrate on SCC of the major salivary glands and paranasal sinuses. In all, 33 cases of paranasal sinus and 43 cases of major salivary gland SCC have been reported in the literature. METHODS: We report two patients, one with submandibular gland SCC and the other with maxillary sinus SCC. A literature review of all known paranasal sinus and major salivary gland SCC with inclusion of data from these two new cases is undertaken. Discussion of all past and present cases concentrates on sites of metastasis, treatment, and survival. RESULTS: Paranasal sinus SCCs predominantly arise from the nasal cavity, whereas the parotid gland is the primary site in three fourths of major salivary gland SCCs. One half of major salivary gland and three fourths of paranasal sinus SCCs have only local disease at presentation. Both patients in this report developed bone marrow metastases, a feature heretofore not observed in SCC from these primary sites. The patient with maxillary sinus SCC developed the syndrome of inappropriate antidiuretic hormone (SIADH). CONCLUSION: The paranasal sinus and major salivary glands are rare primary sites for SCCs. Long-term survival with local therapy in patients with local disease can occur, but in patients with metastatic disease survival mirrors metastatic pulmonary SCC.

Bone Marrow↗