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Biomedical subjects

M L Cook

Publications and source records attributed to M L Cook.

43 records · Page 3Linked to original sources
PubMed1971

Latent herpes simplex virus in spinal ganglia of mice.

Herpes simplex virus establishes a persistent, latent infection in spinal ganglia after mice have recovered from posterior paralysis. Infectious virus is replicated when these ganglia are explanted and maintained as organ cultures in vitro.

Animals↗
PubMed1971

Restriction of herpes simplex virus by macrophages. An analysis of the cell-virus interaction.

Macrophages restrict herpes simplex virus replication and can prevent the development of herpetic disease in mice. In an attempt to define the nature of this restriction, an analysis of virus-specified macromolecular syntheses in infected macrophages was undertaken. The significant results were the following: All cells were killed, but the infection was considered to be abortive since the level of infectious virus in macrophage cultures dropped steadily to a level beyond detection by 25 hr after infection. This restriction appeared to be specific for macrophages; the virus replicated efficiently in other mouse cells. DNA with a density characteristic for herpes simplex virus DNA was extracted from infected cultures, and the proportion of macrophages synthesizing DNA increased from less than 1% to greater than 50% by 6 hr after infection. Studies employing polyacrylamide gel electrophoresis indicated that the major viral-specific proteins were induced in macrophage cultures. In addition, all cells showing cytopathic changes characteristic of herpes virus infection also contained viral antigens which could be detected by fluorescent antibody techniques and, by 15 hr after infection, most contained nascent capsids lacking central dense cores. It is suggested that an error in DNA metabolism may be the primary cause of restriction.

Animals↗
PubMed1968

Labile coat: reason for noninfectious cell-free varicella-zoster virus in culture.

Experiments designed to determine why cell-free varicella-zoster virus replicated in cell culture is noninfectious were performed. Electron micrographs in which varicella-zoster virus (a herpesvirus) was compared to herpes simplex virus in primary human amnion cell cultures showed that the viruses were morphologically indistinguishable inside the nucleus. However, extranuclear varicella-zoster viruses were distinguished from herpes simplex virus by the presence of pleomorphism, incomplete coats, and a resultant loss of central dense cores. This result indicates that varicella-zoster virus possesses a labile coat which is degraded outside the nucleus. It is suggested that the labile coat is a principal reason for the lack of cell-free infectious virus in this system.

Amnion↗
PubMedDate not supplied

Encouraging research in home care: learning from agency staff and family caregivers.

It is clear that research is an increasing role of managers and is crucial to the financial and clinical success of a home health agency. This article has described the important considerations and approaches managers must consider to integrate research effectively. By following one agency's involvement with a researcher on a project, strategies emerge that managers can use to integrate research with staff in everyday practice.

Caregivers↗