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Biomedical subjects

M L French

Publications and source records attributed to M L French.

51 records · Page 3Linked to original sources
PubMed1972

Immunofluorescence staining of group B coxsackieviruses.

Studies were conducted on the sensitivity and specificity of indirect fluorescent-antibody (FA) staining for identification of group B coxsackieviruses. Antisera produced in four different species (monkeys, rabbits, horses, hamsters) and immune ascitic fluids prepared in mice were compared for suitability in FA staining. The horse antisera showed high titers of nonspecific staining, and the rabbit antisera showed relatively low homologous FA titers. Immune reagents from monkeys, hamsters, and mice were used for homologous and heterologous testing against cell cultures infected with the various group B coxsackieviruses. Antisera or immune ascitic fluids produced in these three species showed some heterotypic and nonspecific staining at low dilutions, with the monkey antisera showing the highest heterotypic titers. However, the immune reagents could be diluted to a point where they gave no heterotypic reactivity, but still showed characteristic homotypic staining. Heterotypic staining appeared as diffuse, low-level staining of the cells, whereas homotypic staining revealed characteristic, brightly staining aggregates of viral antigen in the cytoplasm of the infected cells. By using hamster immune sera, appropriately diluted to eliminate heterotypic staining and yet give strong homotypic staining, it was possible to identify correctly 79 (93%) of 85 field strains of group B coxsackieviruses at the first passage level in BS-C-1 cells; the remainder of the strains were identified after two passages in BS-CS-1 cells. No incorrect identifications were made. A limited number of field strains of group B coxsackieviruses were passed into rhesus monkey kidney and human fetal diploid kidney cells, and these were all correctly identified by FA staining, even the strains which failed to produce a cytopathic effect in the human fetal diploid kidney cells. Two human heart and brain tissues from which coxsackievirus type B4 had been isolated failed to show homotypic FA staining in excess of nonspecific or heterotypic staining.

Animals↗
PubMed1971

Contamination of sputum induction equipment during patient usage.

Sputum induction equipment was evaluated for its capacity to become contaminated by patients harboring Mycobacterium tuberculosis. The mouthpiece, goose-neck, and 10% NaCl solution were found capable of being contaminated by tuberculous patients. Two per cent glutaraldehyde was shown to be an effective means of decontamination. This study indicates that the entire induction apparatus must be cleaned and decontaminated between patients.

Aldehydes↗
PubMedDate not supplied

Incidence of positive serologic tests for treponemal infection in healthy rabbits.

Sera from 167 apparently normal rabbits were screened by the Venereal Disease Research Laboratory (VDRL) slide test and by the fluorescent treponemal antibody-absorption (FTA-Abs) and microhemagglutination assay for Treponema pallidum antibodies (MHA-TP) test. Three sera (1.8%) were reactive in all three tests, a fact suggesting possible inapparent infection with Treponema paraluis-cuniculi. One hundred forty-four sera (86.2%) were nonreactive in all tests. The VDRL slide test was reactive with 22 sera (13.2%). In 19 of these, treponemal tests were nonreactive; thus the rabbits probably were not infected with pathogenic treponemes. One hundred sixty-three sera (97.6%) were nonreactive in both treponemal tests, and three sera (1.8%) were reactive in both. One serum was reactive in the FTA-Abs test and nonreactive in the MHA-TP test. The lower incidence of reactivity with treponemal tests suggests that the FTA-Abs and MHA-TP tests are more useful than the VDRL slide test for screening rabbits intended for experimental studies of syphilis.

Animals↗
PubMedDate not supplied

Women at risk for gonorrhea: comparison of rosaramicin and ampicillin plus probenecid in the eradication of Neisseria gonorrhoeae, Chlamydia trachomatis and genital mycoplasmas.

Rosaramicin is a macrolide antibiotic with activity against Neisseria gonorrhoeae, Chlamydia trachomatis, and the genital mycoplasmas Ureaplasma urealyticum and Mycoplasma hominis. Its efficacy in the treatment of genital infections was evaluated and compared with that of single-dose ampicillin plus probenecid in women with known, or suspected, uncomplicated gonococcal infection. Isolation rates for N. gonorrhoeae, C. trachomatis, U. urealyticum, and M. hominis were 72%, 44%, 95%, and 65%, respectively. Rosaramicin cured 24 (89%) of 27 gonococcal infections and 11 (92%) of 12 chlamydial infections. It transiently reduced the carriage of U. urealyticum but had little effect on carriage of M. hominis. Rosaramicin may be of value in the treatment of concurrent gonococcal and chlamydial infections when tetracycline is contraindicated.

Adolescent↗
PubMedDate not supplied

The surgeon's garb.

A comprehensive evaluation of all factors associated with the surgeon's garb worn with and without laminar air flow revealed that in a conventional operating room, all personnel should wear an impermeable one-piece suit with foot attachments, any type of head cover, and, if they are going to be near the operative site or instrument table, a disposable mask. Scrub personnel should wear some type of a disposable gown to reduce the amount of surface contamination. The best way to reduce the environmental and surface contamination would be for all personnel to wear some type of a hooded exhaust equipment, but this is impractical both for circulating nurses and for anesthesiologists. Despite the reduction obtained from all the above controls, the reduction in the amount of bacterial contamination would not be as great as that obtained with laminar air flow, 93% (p < 0.005). Laminar air flow produced the greatest single reduction in environmental and surface contamination and variables such as time, people and attire did not influence the overall counts. The attire recommended for the conventional operating room can be used in laminar air flow with an expectation of overall reduction in contamination.

Antisepsis↗