PubMed Health⌕ Search

Biomedical subjects

M L Khatenever

Publications and source records attributed to M L Khatenever.

11 recordsLinked to original sources
PubMed1979

[Phage typing and lysogen typing of Staphylococcus aureus].

A comparison was made between the results of phage and lysogenic typing of S. aureus strains isolated during several outbreaks of staphylococcal infection and S. aureus cultures isolated from the same carriers at different periods. The study of the groups of strains having the same origin showed that the differences in the number of reactions were more pronounced in lysogenic typing than in phage typing. For this reason lysogenic typing can be recommended only for the identification of those strains which cannot be identified with the use of the phages of the International Basic Set. The results of the experiments with induced phages proliferating in a restriction-defective strain indicated that restriction and modification were mainly responsible for the specificity of lytic reactions.

Animals↗
PubMed1978

[Transfer of drug resistance plasmids from Staphylococcus epidermidis to Staphylococcus aureus in mixed culture].

A possibility of transfer of chloramphenicol-resistance and penicillin-resistance plasmids from 4 different donor S. epidermidis strains to 2 S. aureus strains was demonstrated. Chloramphenocol-resistance plasmid was found in S. epidermidis 1065/77 which was not expressed in this strain but could be transferred to and was expressed in Staphylococcus aureus strains. Penicillin-resistance and chloramphenicol-resistance plasmids were transferred simultaneously in 40 per cent of the colonies.

Chloramphenicol↗
PubMed1977

[Drug resistance of hospital strains of Staphylococcus epidermidis belonging to different biological types].

A total of 567 strains of Staph. epidermidis isolated from the bacteria carriers in several surgical units were studied. The cultures were typed according to the method developed by the authors. Their resistance to 12 antibiotics, mercuric chloride and cadmium sulphate was determined. Comparison of the strain biotypes and their drug resistance showed in some cases statistically significant correlation. It should be especially noted that staphylococci with pronounced polyresistance (simultaneous resistance to 7--12 antibiotics) prevailed among the strains belonging to biotypes 1 and 3.

Carrier State↗
PubMed1977

[Spontaneous mutants of S. aureus 209 P resistant to 3,6-diaminoacridines].

Spontaneous mutants of S. aureus 209 P resistant to proflavine sulphate and possessing cross resistance to acriflavine and acridine yellow were isolated. Sensitivity of these mutants to acridine orange, acridines No. 40 and 56 remained unchanged. The phage type and the indices of potential pathogenicity of proflavine-resistant mutants coincided with the corresponding S. aureus 209 P indices.

Acridines↗
PubMed1976

[Capsule formation in Staphylococcus aureus of hospital origin].

The authors elaborated suitable conditions for cultivation of freshly isolated Staph. aureus, which offered a possibility of detection among them of a high percentage (75.2) of capsular cultures. It was shown that when grown in serum semifluid agar capsular staphylococci formed diffuse colonies of three different types. No correlation between the presence of the capsule, on the one hand, and the absence of the flocculus-forming factor and resistance to the type bacteriophage, on the other hand, was revealed in the Staph. aureus strains.

Cell Wall↗
PubMed1976

[Data on the identification of staphylococci of hospital origin. Report 2. Method of biological typing of S. epidermis].

In accordance with the principles of numerical taxonomy the authors elaborated a new method of biological typing of S. epidermidis strains of hospital origin based on the use of 5 tests (gelatinase, hemolysis, lecithinase, phosphatase, lysozyme). The suggested scheme includes 5 biotypes. In typing 690 S. epidermidis strains it was revealed that 47.5% belonged to biotype 1,14.4% - to type 2,11.2% - to type 3,7.7% - to type 4,14.5% - to type 5, and 4.7% were nontyping strains. The suggested scheme permitted better differentiation of the cultures under study than methods of biological typing suggested earlier by foreign authors. The new scheme requires wide approbation.

Bacteriological Techniques↗
PubMed1976

[Indentification of staphylococci of hospital origin. I. Specific identification of staphylococci].

In 22 biological tests a study was made of the properties of 1117 strains of staphylococci isolated from patients and medical personnel surgical departments. The significance of each of the tests for species identification of staphylococci was assessed on the basis of correlation of its results with the results of study of 3 main signs characteristic of S. aureus: the presence of coagulase, anaerobic mannite fermentation, and of DNA-ase. Besides the ones pointed out the following could be considered as properties characteristic of S. aureus: flocculus-forming factor, fibrinolysin, hyaluronidase, lysozyme, golden pigment, tellurite-reductase, aerobic fermentation of mannite and tregalose. A standard system of species identification of staphylococci was elaborated; on its basis assessment was made of the diagnostic value of a number of simple systems used in practice for determination of staphylococcus species.

Coagulase↗
PubMed1975

[Antistaphylococcal bacteriostatic activity of the sera from surgical patients].

Blood serum was examined in 41 patients (operated for various forms of acute appendicitis) on the 5th--6th day after the operation when 10 of the patients developed purulent complications of staphylococcal etiology. A determination was made of the titres of beta-lysins and the bacteriostatic activity against 2 strains of S. aureus by the modified Ehrenkranz et al method. The mentioned indices were low in the great majority of cases; consequently, the appearance of purulent complications in the operated patients was independent of the antistaphylococcal bacteriostatic activity and the beta-lysin titres in their sera. Prophylactic express-immunization of a number of patients by crude staphylococcus toxoid did not influence the nonspecific humoral immunity factors.

Acute Disease↗