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M L Pedro-Botet

Publications and source records attributed to M L Pedro-Botet.

30 records · Page 2Linked to original sources

[Usefulness of the API ZYM system for the identification of Legionella sp].

BACKGROUND: The identification of Legionella genus is usually difficult, time consuming, and expensive. We decided to study the usefulness of API-ZYM system (Biomerieux) for Legionella species identification. METHODS: A total of 96 strains (82 from Legionella genus and 14 from other bacterial genus) were included. We studied 19 different enzyme activities and we proceed following the manufacturers directions, with an incubation time for galleries of 4 hours at 37 degrees C. RESULTS: Seventeen out of the 19 enzyme activities were uniformly positive or negative for Legionella sp. Thus, the sensitivity of these activities was 100% in identifying Legionella sp. Valine and cystine-arylamidase gave variable reactions for Legionella sp. strains. When the results of these enzyme test were compared to those of the control strains (Haemophilus influenzae, Pseudomonas aeruginosa) we observed that alkaline phosphatase, esterase, esterase-lipase, acid phosphatase and naphthol-AS-BI-phosphohydrolase in one side and esterase, esterase-lipase and leucin-arylamidase in the other side were able to differentiate Legionella sp. strains from P. aeruginosa and H. influenzae, respectively. CONCLUSION: API SYM system is a simple, accurate and reproducible method for the identification of Legionella sp.

Bacterial Proteins↗

[Bacteremia after fibrobronchoscopy. Prospective study].

We prospectively evaluated in 88 inpatients the incidence of fever and bacteremia after fiberoptic bronchoscopy and its predisposing variables. Fever was present in 27% of cases and no bacteremia could be demonstrated neither associated nor intercurrent . Chronic Hepatic Disease, endobronchial pathology and bronchial biopsy practice were predisposing factors for fever development in the univariant analysis.

Adult↗

[Haemorrhagic exanthema due to dengue virus induced by acetylsalicylic acid].

Dengue fever, a viral infectious disease characteristic of tropical climates, is considered to be a re-emergent pathology responsible for several serious outbreaks in the last decade. Some factors have been involved in the spread of the virus and its vectorial mosquito carrier: human alteration of the ecosystems, improvement and speed in the transit of goods and people and climate changes. As a reflection of this, an increase in imported cases is probable, especially in tourists coming from endemic areas, considering its short period of incubation (7-10 days). The recognition of personal antecedents of journeys, the main symptoms of the disease and the potential presence of complications (haemorrhagic dengue) should be included in the examination of fever of unknown origin or feverish exanthema. The case of a patient is presented whose clinical picture of classic dengue fever was worsened by self-treatment with acetylsalicylic acid.

Anti-Inflammatory Agents, Non-Steroidal↗