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Biomedical subjects

M Labuda

Publications and source records attributed to M Labuda.

At least 73 records · Page 4Linked to original sources

[Long-term results of surgical treatment of portal hypertension].

Large number of contradictory operative surgeries refer best to embarrasments, existed on this section of surgery. Today, from the amount of operative surgeries prevail the endoscopic sclerotization of the gastroesophageal varices. On basis of retrospective study of 48 portosystemic shunts was showed, that 28 operated patients survive average 8.1 year, till now 4 patients of them are alive, within 8-14 years. In 7 cases of azygoportal deconexion by Torres-Dengi, average time of survive was 2.5 year. There experiences led the authors to elaborate an algorithm of treatment of portal hypertension, where the postosystemic shunts are the portosystemic shunts are the effective palliative operative surgery in indicated cases.

Esophageal and Gastric Varices↗

Serological survey with the antigen of haemorrhagic fever virus with renal syndrome in small rodents in Slovakia.

Results of serological survey in small rodents with the antigen of haemorrhagic fever with renal syndrome (HFRS) virus indicate the existence of a natural focus of this virus in Slovakia. Antibodies were found in the following free-living rodent species: Clethrionomys glareolus, Apodemus agrarius, Apodemus sylvaticus, Pitymys subterraneus, Microtus arvalis and Microtus economus. A total 10 out of 120 (8.2%) small rodents trapped in investigated localities of Western and Eastern Slovakia had antibodies to the HFRS virus antigen as detected by complement fixation (CF) and/or immunofluorescence (IF) tests.

Animals↗

Variations of four Aedes aegypti mosquito strains in their susceptibility to and transmissibility of Tahyna virus.

Aedes aegypti mosquitoes originating from different colonies were infected with Tahyna virus by sucking on viraemic newborn mice (peak titre 10(2) X 5 mouse i.c. LD50/0.01 ml). The mosquitoes of laboratory strain "London" proved most susceptible (infection rate 74.2%, transmission rate 60%), while mosquitoes of the laboratory strain "Basel" and strain "Bangkok" (Southeast Asia) were less susceptible (infection rate 23.7 and 17.6%, respectively, transmission rate 6.0 and 10.0%, respectively). Mosquitoes of "Ifakara" strain (East Africa) were found resistant to Tahyna virus infection.

Aedes↗

Haemorrhagic fever virus with renal syndrome in small rodents in Czechoslovakia.

The antigen of haemorrhagic fever with renal syndrome (HFRS) was detected in the lungs of the following free-living small rodents trapped in different localities in Eastern Slovakia: Clethrionomys glareolus (2 positive samples of 7), Apodemus flavicollis (1 sample of 24) and Apodemus agrarius (7 positive samples of 66). The virus was first identified by indirect fluorescent antibody (FA) staining using human convalescent serum from a case of epidemic nephropathy (NE) of Scandinavia. The lung suspensions selected according to positive immunofluorescence were inoculated by intramuscular (i.m.) route into suckling rats; the antigen prepared from the lungs of these rats by sucrose-acetone extraction reacted with the prototype human serum in complement-fixation (CF) reaction. The results of the latter assay were in good agreement with those of the indirect FA staining.

Animals↗

Experimental model of transovarial transmission of Tahyna virus in Aedes aegypti mosquitoes.

The progeny of 31 viruliferous Aedes aegypti females infected with Tahyna virus by sucking on viraemic newborn mice was investigated for virus presence. Out of 1587 individuals of the F1 generation, 16 suspensions representing the progeny of 7 females were positive in 146 trials. Individuals of the F1 generation failed to transfer the virus by sucking. Electron microscopy revealed the presence of Tahyna virus particles in the cytoplasm of maturing oocytes inevitably confirming the transovarial transfer of the virus by germinal cells of Aedes aegypti mosquitoes.

Aedes↗

Bionomics and health importance of fowl tick Argas (Persicargas) persicus (Oken, 1818) (Ixodoidea: ARgasidae).

The fowl tick Argas (Persicargas) persicus occurs in warm areas of Slovakia, where its distribution is limited by the 650-700 mm annual average isohyet and the +8 degrees C annual average isotherm. In Slovakia, larvae hatch from spring to autumn, and nymphal and adult moultings occur chiefly in July and August. Larvae feed for four days, nymphs and adults for less than one hour. The tick develops and reproduces during the warm season in the wild but throughout the year under favourable laboratory conditions in which the life cycle requires 17 months.

Animals↗

Isolation of Tettnang virus from ticks, mosquitoes and small rodents.

Seven strains of Tettnang virus were isolated in Czechoslovakia: 3 from Ixodes ricinus tick nymphs, males and females, one from Dermacentor reticulatus tick females, one from Aedes cantans mosquitoes and two strains from the brains of Clethrionomys glareolus and Apodemus flavicollis small rodents.

Aedes↗