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M Lampe

Publications and source records attributed to M Lampe.

23 records · Page 2Linked to original sources
PubMed1977

Cytochemical variation of mast cells in the skin of the rat.

The mast cells of the skin of the external ear of the rat have been examined, with a view to the possible identification of different cell-types. It was not possible to discern more than one type of cell using criteria of size, shape, nuclear chromophilia or sizes of granules. The cytoplasmic granules of all of the cutaneous mast cells were tingible with Alcian blue (pH. 1.0 and 2.5) and, metachromatically, with toluidine blue 0. These properties were attributable to the heparin contained in the granules. The cells all gave positive reactions with the diazosafranine method, possibly by virtue of their content of serotonin. About two thirds of the mast cells in the dermis contained granules which were stainable by the periodic acid-Schiff (PAS) method and which could bind concanavalin A. It is suggested that these latter cells contain, in addition to heparin, a neutral mucosubstance characterized by a high content of alpha-d-glucosyl and/or alpha-d-mannosyl monosaccharide residues. The PAS-positive cells were relatively more abundant in vascular adventitiae and nerves and less so in the general connective tissue of the dermis than were the mast cells which did not contain the second mucosubstance.

Animals↗
PubMed1976

Degranulation of dermal mast cells: effects of fixation and of antidromic nervous impulses on two histochemically identified cell-types.

Two types of mast cell can be identified histochemically in the dermis of the rat's external ear. One type is recognized by the binding of concanavalin A (con A) to the cytoplasmic granules (con A-positive cells) while in the other type (con A-negative cells), the granules do not bind con A. The granules in both types are stained metachromatically by toluidine blue. Antidromic stimulation of the great auricular nerve for 2 min results in an increased proportion of degranulating mast cells in the auricular dermis and both types of cell are affected to an approximately equal extent. In discussion of this observation, it is argued that both the con A-positive and the con A-negative mast cells are probably involved in the mediation of vasodilatation due to axon reflexes in injured skin. The proportions of degranulating mast cells determined in histological preparations varied with the fixatives (Carnoy and glutaraldehyde-formaldehyde) used, but the increased degranulation due to antidromic nervous stimulation could be detected after either fixation.

Animals↗
PubMedDate not supplied

Lack of correlation after reperfusion between ventricular function and infarct size estimated by thallium single-photon emission computed tomography.

In 32 patients with acute myocardial infarction, who had undergone successful intracoronary thrombolysis, the results of regional wall motion measured from contrast cineangiograms 10 to 21 days after thrombolysis were related to the results of thallium single-photon emission computed tomography (SPECT) after intravenous dipyridamole. Wall motion was measured by means of the centerline method, and thallium defect size was estimated by comparing the patient's circumferential profile with that of 20 normals. No correlation was found between ejection fraction or regional wall motion and thallium defect size. The time from symptom onset to thrombolysis was inversely correlated with the degree of hypokinesis (r = -0.51) but not with thallium defect size. In patients treated within 3 hours, hypokinesis was significantly less than in patients treated later (-1.1 +/- 0.6 SD vs -2.2 +/- 0.8 SD, p less than 0.01) whereas thallium defect size was not significantly different in both groups. It is concluded that, in patients after thrombolysis, thallium defect size determined by SPECT does not reflect the degree of left ventricular dysfunction.

Adult↗
PubMedDate not supplied

The role of spermatozoa in the pathogenesis of Chlamydia trachomatis salpingitis in a primate model.

BACKGROUND AND OBJECTIVES: The role of spermatozoa in carrying microorganisms from the cervix to the fallopian tubes and promoting infection was examined in eight pig-tailed macaques. GOAL OF THIS STUDY: The aim of this study was to determine whether spermatozoa play a role in the transport of Chlamydia trachomatis into the upper reproductive tract and in the pathogenesis of chlamydial salpingitis. STUDY DESIGN: Five monkeys were inoculated with Chlamydia trachomatis in the cervix and mated, and three other monkeys were infected in the cervix but not mated. RESULTS: Culture positive results were obtained for cervical samples from all animals. After cervical inoculation, no mated animals and one unmated animal had positive culture results for samples from the fallopian tubes. After mating, no spermatozoa examined in samples taken from the upper tract or cul de sac had elementary bodies (EBs) on their surfaces, and only one sample obtained from the cervix showed C. trachomatis attached to the spermatozoa, as determined by fluorescent antibody (FA) staining. Two of the mated monkeys became pregnant; one spontaneously aborted late in gestation, and the other delivered a normal female infant. The fallopian tubes of all eight monkeys were challenged directly with serovar D and examined for evidence of salpingitis. Repeat laparoscopy was performed to monitor the inflammatory response. Tubal specimens for isolation and polymerase chain reaction (PCR) were obtained. Hysterectomies were performed between 3 and 4 weeks after tubal inoculation. Histopathologic examination of the fallopian tubes revealed widespread inflammation consisting of focal collections of lymphocytes and plasma cells in the submucosa. Peritubal adhesions were observed in four of five mated monkeys and in two of three unmated monkeys. CONCLUSION: This study failed to confirm that spermatozoa play a role in the pathogenesis of C. trachomatis infection.

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