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Biomedical subjects

M Lederer

Publications and source records attributed to M Lederer.

At least 19 recordsLinked to original sources
PubMed1989

Adsorption chromatography on cellulose. IV. Separation of D- and L-tryptophan and D- and L-methyltryptophan on cellulose with aqueous solvents.

Differences in RF values between D- and L-tryptophan on cellulose paper, developed with water, were observed already in 1954 and now several variables of this separation, such as modifications to the tryptophan molecule, the temperature and salting-out have been examined. Differences were found in the mechanism of the process compared with the systems described by Yuasa et al. [Chromatographia, 21 (1986) 79], who separated amino acid enantiomers on cellulose with pyridine-ethanol-water mixtures.

Chemical Phenomena↗
PubMed1989

The role of chromatography and electrophoresis in biomedical sciences.

Various facets of the history of clinical chromatography are considered, with emphasis on their application to the present state of the art. The work of Goppelsroeder on capillary analysis is reviewed, followed by a discussion of C.E. Dent's work on aminoacidurias, Lato's thin-layer chromatographic studies of sugars in urine, Ian Bush's approach to automated chromatography, as well as the extensive possibilities of high-voltage electrophoresis shown by Clotten and Clotten. It is interesting that none of these methods has been applied extensively in diagnostic work. Capillary electrophoresis, which was developed by Hjertén in 1969, seems to have gained new interest recently. At present, although it seems obvious that chromatographic methods could solve numerous diagnostic problems, surprisingly little work is being done outside the larger hospital laboratories. Some of the reasons for this situation are discussed, as are the future prospects of the Biomedical Applications section of the Journal of Chromatography.

Chromatography↗
PubMed1988

Adsorption chromatography on cellulose. II. Separations of aromatic amino acids, biogenic amines, alkaloids, dyes and phenols and determination of hydrophobic constants.

Separations of amines, phenols, alkaloids, etc., using cellulose papers or thin layers with aqueous ammonium sulphate as eluent are reported. These separations illustrate that excellent analytical separations are possible and provide data for the study of the mechanism of adsorption on cellulose from aqueous solutions.

Alkaloids↗