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M Lipińska

Publications and source records attributed to M Lipińska.

13 recordsLinked to original sources
PubMed1993

[Studies of epitope specificity of polyclonal antibodies against Proteus mirabilis R mutants].

The chemical structure of the following P. mirabilis R mutants lipopolysaccharide (LPS) were already established: R110/1959 (Ra), R4/028 (Rc) and R45/1959 (Re). In this report we focus on P. mirabilis R5/O28, R13/1959 and R14/1959 and R14/1959. The last one corresponds to Salmonella transient forms, and synthesis truncated core oligosaccharide lacking terminal DD-Hep and nevertheless substituted by T polysaccharide whose structure occurred to be similar to P. penneri 42 O-repeating unit. The knowledge of chemical structure of P. mirabilis R mutants lipopolysaccharides led us to the study of the epitope specificity of rabbit polyclonal R specific antisera. The results show strong structural and serological relatedness of LPS from P. mirabilis R110 and R13. Antibodies against P. mirabilis R4 recognize in homologous LPS an epitope sharing oligosaccharide Glc-Hep. The serological studies revealed also close similarities of LPS from P. mirabilis R14 and P. mirabilis S1959, O28 as well as P. penneri 42. These data indicate that polyclonal antibodies against P. mirabilis R14 are directed against four epitopes: two in T-polysaccharide (D-Glc-(beta 1,4)-D-Glc and terminal GalA residue) and two in core oligosaccharide (D-Glc-(alfa 1,6)-D-Glc and terminal GlcNAc residue) of lipopolysaccharide molecule.

Animals↗
PubMed1993

[Further types of core lipopolysaccharides in Proteus].

Comparative analysis of the chemical composition of 11 core oligosaccharides isolated from lipopolysaccharides of the wild (S) and phenotypically rough (R) strains Proteus mirabilis (nine) and Proteus vulgaris (two) allowed to recognize three new types Proteus core, classified as IV, V, VI. All of them contained D-galactose and D-galactosamine in addition to common core constituents: D-glucose, D-galacturonic acid, L-glycero-D-manno-heptose, KDO, EtN described for Proteus core types I, II, III (6, 7, 8). D-glucosamine was characteristic for Proteus core type VI whereas D-glycero-D-manno-heptose for types V and VI.

Oligosaccharides↗
PubMed1993

[Immunochemical studies of O-specific polysaccharide from Proteus penneri 14 lipopolysaccharide].

O-specific polysaccharide was obtained on mild acid degradation of Proteus penneri strain 14 lipopolysaccharide and found to contain equimolar of D-galactose, D-ribose, 2-acetamido-2-deoxy-D-glucose, N-(D-galacturonoyl)-L-alanine and 3-(Nacety-L-alanyl)-amido-3,6-dideoxy-D-glucose. On the basis of non-destructive NMR analysis it was concluded that repeat unit of the 0-specific polysaccharide of P. penneri 14 has the following structure: -2-beta-D- Quip3NAlaAc-(1-->4)-alfa-D-GalpAAla-(1-->2)-beta-D- Ribf-(1-->4)-beta-D-Galp-(1-->3)-beta-D--GlcpNAc-(1--> This structure was confirmed by structural elucidation of trisaccharide and disaccharide fragments prepared on mild acid hydrolysis of the polysaccharide. Immunodominant role of the partial structures of the pentasaccharide repeating unit in manifesting serological specificity of P. penneri 14 was discussed. Very weak cross-reactions of P. penneri anti-serum were observed with E. coli 0114 and Shigella boydii 08 LPS's, which showed some structural similarities. No cross-reaction with P. mirabilis 027 LPs was detected.

Antigens, Bacterial↗
PubMed1981

Studies on the role of humoral and cell-mediated immunity in pigs after vaccination with Aujeszky's disease virus.

Humoral and cellular immunity in pigs vaccinated twice with Aujeszky's disease virus (ADV) was studied by seroneutralizing test and direct leucocyte migration inhibition technique. Significant migration inhibition of leucocytes (LMI) was found on the fifth day, whereas specific antibodies began to appear at that time only in very low titers. Anamnestic reaction due to the second injection of ADV did not bring about a significant increase of migration inhibition of leucocytes, instead the level of antibodies elevated markedly.

Animals↗
PubMed1978

Immunological experimental arthritis in pigs. I. Propagation dynamics of the attenuated strain of Aujeszky's disease virus in the pig organism and the humoral immunity formation.

Studies on the propagation dynamics of Aujeszky's disease virus and the formation of general and local joint immunity were to provide information explaining the mechanism of experimental rheumatoid arthritis in animals. It was shown that: Aujeszky's disease virus administered intramuscularly caused viremy on 3-4th day after its application and disappeared from the blood circulation on 5-6th day, introduced into the pig ankle joint, the virus was eliminated from the articular fluid two hours after injection, but it appeared again 3-7 days later probably due to the propagation in the cells of the synovial membrane, and that first parenteral administration of the virus induced the formation of neutralizing antibodies in low titer (5-10 units); application of the virus for the second time brought about a considerable increase of antibodies titer within 40-80 units.

Animals↗
PubMed1978

Immunological experimental arthritis in pigs. II. The level of complement in the pigs immunized with the Erysipelothrix rhusiopathiae and virus of Aujeszky's disease.

The level of complement was studied in 3 groups of pigs. One group was immunized with Erysipelothrix rhusiopathiae and two remaining groups with virus of Aujeszky's disease. The lowest titers, irrespective of the starting level were shown to exist in all the studied groups in the third week after the last dose of the virus.

Animals↗
PubMed1978

Immunological experimental arthritis in pigs. III. Inflammatory reactions in the brain of pigs immunized with the attenuated virus of Aujeszky's disease.

Histopathological brains and spinal cords of pigs were examined at different times of immunization with the virus of Aujeszky's disease (AD). Temporary inflammatory reaction was found to have the features of non-suppurative disseminated inflammation in the second week after the second virus dose had been given. Perivascular and subependymal lymphocytic infiltrations were observed and the focal multiplication of microglia.

Animals↗
PubMed1978

Immunological experimental arthritis in pigs. IV. Reaction of the synovial membrane of the non-immunized and immunized pigs after intraarticular administration of the virus of Aujeszky's disease.

7 days after intramuscular administration of the attenuated virus of Aujeszky's disease, strain TK 300 L, it was found: in non-immunized animals--immunological arthritis with fibrinoid necrosis and abundant infiltrations from lymphoid cells; in animals of low antibodies titer--only few infiltrations from lymphoid and plasmic cells round the sub-synovial vessels; in animals of high antibodies titer--vast areas of fibrinoid necrosis, abundant infiltrations from lymphoid cells and presence of gigantic cells.

Animals↗
PubMed1978

Immunological experimental arthritis in pigs. V. Reaction of the synovial membrane in the pigs non-immunized and immunized with the virus of Aujeszky's disease after the intraarticular administration of the immunological complexes.

Immunized animals were given intraarticular complexes formed in vitro from the autologous serum and virus AD. 7 days after complex administration with excess of the virus, weak inflammatory reactions of the immunological type were noted. After neutral complex administration virulent immunological inflammation of the synovial membrane took place. The histological picture resembled rheumatoid arthritis in the man and the changes obtained after the virus administration to the joints with a high level of antibodies. Administration of complexes and homological serum alone to the joints of the nonimmunized animals caused the occurrence of superficial focuses of fibrinoid necrosis, but there was a lack of cellular reactions.

Animals↗