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M Lock

Publications and source records attributed to M Lock.

At least 37 records · Page 2Linked to original sources
PubMed1998

New High-Resolution Analysis of the nu3, nu4, and nu6 Bands of D2CO Measured by Fourier Transform Spectroscopy

A reanalysis of the nu3, nu4, and nu6 interacting bands of D2CO has been carried out in the region 850-1250 cm-1 using high-resolution Fourier transform spectra recorded at Giessen. As compared to the previous study of these bands (1987, K. Nakagawa, R. H. Schwendeman, and J. W. C. Johns, J. Mol. Spectrosc. 122, 462-476) higher J and Ka transitions were assigned for the three bands, leading to a better determination of the upper state constants. The v3 = 1, v4 = 1, and v6 = 1 experimental energy levels were introduced in a least-squares fit calculation together with the microwave measurements available in the literature in order to obtain the upper state parameters (band centers, rotational and coupling constants). In this calculation, which allowed us to reproduce both the infrared and the microwave measurements to within their experimental accuracies, the A-, B-, and C-type Coriolis interactions involving the rotational levels belonging to the v4 = 1 and v6 = 1, v3 = 1 and v4 = 1, and v3 = 1 and v6 = 1 interacting states respectively were explicitly taken into account. Finally, from the intensities, a new determination of the relative values of the q3, q4, and q6 first derivatives of the D2CO dipole moment was performed. Copyright 1998 Academic Press. Copyright 1998Academic Press

Journal Article↗
PubMed1998

Measuring attitudes towards smoking in the Community Intervention Trial for Smoking Cessation (COMMIT).

We present the development of indices using baseline data from the Evaluation Survey for the Community Intervention Trial for Smoking Cessation (COMMIT). The indices are designed to measure two primary attitude constructs that relate to smoking behavior: beliefs about smoking as a public health problem (SPHP); and norms and values concerning smoking (NVS). Two general approaches to index construction, the rational method and the factor analytic method, were used. Item analysis suggested good internal consistency for both indices (alpha > 0.75). Seven subconstructs emerged from the factor analysis accounting for 55.0% of the total variance. The SPHP and NVS items uniquely identify with four factors and three factors, respectively, confirming the validity of the two indices. Confirmatory factor analyses of a different data set provided further validation. Validity was also assessed by an examination of the relationships between index scores and smoking status. Smokers reported significantly higher scores than non-smokers on the two measures indicating, as anticipated, that smokers have more favorable attitudes towards smoking than non-smokers. These findings suggest that the two a priori constructs of SPHP and NVS are empirically distinguishable components of attitudes towards smoking, and that the indices developed here are reliable and valid measures of those constructs.

Adult↗
PubMed1997

Co-localization of HIV-1 Nef with the AP-2 adaptor protein complex correlates with Nef-induced CD4 down-regulation.

The nef gene of human and simian immunodeficiency viruses is critical for AIDS pathogenesis. Its function in vivo is unknown, but in vitro natural isolates of Nef down-regulate expression of the cell surface CD4 molecule, a component of the T cell antigen receptor and the viral receptor, by accelerating its endocytosis. We have used chimeric proteins comprised of the natural HIV-1 NA7 Nef fused to a strongly fluorescing mutant of green fluorescent protein (GFP) to correlate Nef function with intracellular localization in human CD4-positive Jurkat T cells. The NA7-GFP fusion protein co-localizes with components of the clathrin coat, including clathrin and the beta-subunit of the AP-2 adaptor protein complex, at discrete locations that are consistent with the normal cellular distribution of clathrin coats at the plasma membrane. The NA7-GFP protein is also found in the perinuclear region of the cell, which is likely to reflect the Golgi apparatus. Evidence from a CD4-negative fibroblast cell line indicates that co-localization of NA7-GFP with components of the clathrin coat does not require expression of the CD4 molecule. Analysis of a large panel of chimeric molecules containing mutant Nef moieties demonstrated that the N-terminal membrane targeting signal cooperates with additional element(s) in the disordered loops in the Nef molecule to co-localize the Nef protein with AP-2 adaptor complexes at the cell margin. This localization of NA7-GFP correlates with, but is not sufficient for, down-regulation of surface CD4 and at least one additional function of Nef is required. In T cells co-expressing CD4 and NA7-GFP, CD4 at the cell surface is redistributed into a discrete pattern that co-localizes with that of NA7-GFP. Our observations place NA7-GFP in physical proximity to AP-2-containing clathrin coat at the plasma membrane and imply that Nef interacts, either directly or indirectly, with a component of the AP-2-containing coat at this location. This evidence supports a model whereby Nef recruits CD4 to the endocytic machinery via AP-2-containing clathrin coats at the plasma membrane.

Adaptor Protein Complex alpha Subunits↗
PubMed1997

Aneurysmal bone cyst of the terminal phalanx of the thumb in a child.

We report a case of aneurysmal bone cyst (ABC) of the terminal phalanx of the thumb in a young boy, which was treated successfully with complete curettage with preservation of the growth plate. Three years later he was asymptomatic, with normal growth of the thumb, full range of movement and no evidence of recurrence. For this rare benign cyst in a child's hand, we recommend simple surgery to preserve the growth plate.

Bone Cysts, Aneurysmal↗
PubMed1997

The Bentham Unit: a pilot remand and assessment service for male mentally disordered remand prisoners. I: Clinical activity in the first year, and related ethical, practical and funding issues.

BACKGROUND: Because of continuing concern regarding the inadequacy of existing NHS provision for mentally disordered remand prisoners, the Bentham Unit was commissioned as a pilot project to provide rapid assessment and, where appropriate, hospital admission for such prisoners from the former North West Thames catchment area. METHOD: Information is presented on the 150 referrals and 62 admissions in the first year of the service's operation. RESULTS: The service met the Reed Report targets for assessment time. Most of those referred were previously known to psychiatric services. The alleged offences were more serious than anticipated. Those admitted were transferred to appropriate services at the conclusion of their court cases, and remained in contact with services three months later. Waiting for the conclusion of court proceedings significantly prolonged the length of stay. Substantial cost transfers from the criminal justice system to the health system were evident. CONCLUSIONS: Adequate services for mentally disordered remand prisoners are entirely achievable; provision of such services is a resource issue, not a clinical problem.

Adult↗
PubMed1997

Medicalization of women's third age.

Medicalization usually refers to the process whereby the normal processes of pregnancy, childbirth, menstruation and menopause have been claimed and redefined by medicine. Rather than discussing medicalization and menopause in terms of the number of women taking hormones, or the percentage of physicians convinced they should prescribe them, this paper looks at the visual image of the menopausal woman as portrayed in the pharmaceutical literature and in the mass media. Unlike the depressed and sickly looking women shown in the pharmaceutical advertisements in the 1970s, this 1990s version of the menopausal woman is shown glowing with fitness, with well-maintained teeth, hair and skin, far too fit to break a hip, have a heart attack, or witness the slow destruction of their minds by Alzheimer's disease. This image is not to be confused with the reality of being a menopausal woman, yet the two are intimately intertwined, for the image determines how menopausal women see themselves and how they are seen in the wider society. The final section of the paper discusses how health is the new virtue for women as they age as each individual is held responsible for what happens to her body, particularly in terms of the decisions made at the time of menopause.

Aged↗
PubMed1996

Identification of a set of proteins (C' and C) encoded by the bicistronic P gene of the Indiana serotype of vesicular stomatitis virus and analysis of their effect on transcription by the viral RNA polymerase.

Previous work (C.F. Spiropoulou and S.T. Nichol, 1993, J. Virol. 67, 3103-3110) has demonstrated the existence in cells infected with the New Jersey serotype of vesicular stomatitis virus (VSV) of two small carboxy-coterminal proteins encoded by the P mRNA. These proteins have been named C' and C. We are interested in studying the function of these proteins in the virus life cycle. Toward this end, we have cloned the ORF encoding the potential C' protein of the Indiana serotype as a histidine-tagged fusion protein, purified the expressed protein from Escherichia coli, and used the fusion protein as an immunogen to raise antiserum in a rabbit. We have used this anti-C' protein serum to demonstrate that both of the predicted C' and C proteins are synthesized in cells infected with the Indiana serotype of VSV. In addition we have localized a portion of these proteins to nucleocapsids isolated from infected cells, suggesting that they may play a role in RNA synthesis. Reconstitution of the viral polymerase activity by expressing the L and P protein subunits with or without the C proteins failed to demonstrate any effect of the presence of these latter proteins on reconstituted transcription using purified nucleocapsids as templates. However, we have been able to show a dramatic stimulation of the polymerase activity in purified virions by the addition of purified C' protein to in vitro transcription reactions. Both the level and the fidelity of mRNA synthesis are stimulated by this protein. Evidence for the specificity of this effect comes from the fact that stimulation appears to be serotype specific; C' protein of the Indiana serotype stimulates transcription by purified Indiana serotype virions but has a minimal effect on transcription by purified virions of the New Jersey serotype. We are continuing our studies to determine the mechanism of this stimulation.

Animals↗
PubMed1996

Death in technological time: locating the end of meaningful life.

This article demonstrates how debate about technologically manipulated death is elaborated in radically different forms in the scientifically sophisticated spaces of Japan and North America. Using recent historical materials and contemporary medical, philosophical, and media publications, I argue that the institutionalization and legitimization of "brain death" as the end of life in North America have been justified by a dominant discourse in which it is asserted that if certain measurable criteria are fulfilled, an individual can be declared scientifically dead. In Japan, by contrast, death is interpreted primarily as a social and not an individual event, and efforts to scientifically define the end of life as a measurable point in time are rejected outright by the majority, including many clinicians. The margins between nature and culture are debated in both cultural spaces, but assigned different moral status in the respective dominant discourse.

Anthropology, Cultural↗
PubMed1995

Contesting the natural in Japan: moral dilemmas and technologies of dying.

The paper opens with a discussion about the recognition of "whole-brain death" as the end of life in North America in order to perform solid organ transplants. This situation is contrasted with Japan, where, despite no financial or technological restrictions, brain death is not recognized, and transplants from brain-dead bodies cannot be performed. The Japanese cultural debate over the past twenty-five years about the "brain-death problem" is presented, followed by an analysis of Japanese attitudes towards technological intervention into what is taken to be the "natural" domain, together with a discussion of current Japanese attitudes towards death. This debate is interpreted as one aspect of a search for moral order in contemporary Japan, revealing ambivalence about self and other, Japan and the West, and tradition and modernity.

Advisory Committees↗