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M Lou

Publications and source records attributed to M Lou.

27 records · Page 2Linked to original sources
PubMed1991

Cadmium uptake through the anion exchanger in human red blood cells.

1. The initial rate of Cd2+ uptake in human red cells was measured by atomic absorption spectrophotometry. 2. About 96% of Cd2+ uptake was inhibited by DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid) with IC50 (concentration giving 50% of maximal inhibition) of 0.3 microM and by furosemide with IC50 of 500 microM and was resistant to ouabain and amiloride. This indicates the implication of the [Cl(-)-HCO3-] anion exchanger in Cd2+ uptake. 3. DIDS-sensitive Cd2+ uptake required the presence of external HCO3-. HCO3- ions had a biphasic effect on Cd2+ uptake. Low bicarbonate concentrations were stimulatory, suggesting formation of translocating bicarbonate-cadmium complexes. Higher bicarbonate concentrations were inhibitory, suggesting further bicarbonate complexation with formation of non-translocating species. Depending on the presence or absence of external Cl-, a maximal Cd2+ uptake of 1.7 or 0.37 mmol (l cells)-1 h-1 was observed at bicarbonate concentrations of 15.6 or 11 mM respectively. 4. In the presence of bicarbonate, external Cl- ions strongly stimulated Cd2+ uptake, with linear increase between 70 and 125 mM. This suggests that one translocating species may have chloride as ligand. 5. DIDS-sensitive Cd2+ uptake was modestly inhibited by physiological concentrations of external phosphate and was resistant to external K+, Mg2+ and Ca2+. 6. In conclusion, the anion exchanger is the major transport mechanism for red cell cadmium uptake. Translocating species appear to be monovalent anion complexes of cadmium with HCO3- such as [Cd(OH)(HCO3)2]- and [Cd(OH)(HCO3)Cl]-.

4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid↗
PubMed1990

Influences of glutathione and sulfhydryl containing compounds on aqueous humor outflow function.

Several sulfhydryl reactive compounds have previously been shown to influence aqueous humor outflow facility. The purpose of the current study was to investigate the effect of glutathione depletion on certain of these sulfhydryl actions. Enucleated calf, monkey, and human eyes were perfused via the anterior chamber by the constant pressure (15 mmHg) technique. In calf eyes, perfusion of 10 mM cysteamine produced a small (-23%, P = 0.03) decrease in outflow facility that was also observed after hyaluronidase pretreatment. In contrast, following pretreatment with 1 mM BCNU [1,3 bis(2 chlorethyl)-1-nitrosourea], an inhibitor of glutathione reductase, and 10 mM diamide, a glutathione oxidant, which did not by themselves significantly affect outflow facility, perfusion of cysteamine resulted in an opposite effect--a remarkably large (+90%, P less than 0.001) increase in outflow facility. Other reduced and oxidized sulfhydryl-containing compounds such as cysteine, beta-mercaptoethanol, and glutathione, itself, as well as the non-sulfhydryl reducing agent, ascorbic acid, were substituted for cysteamine in this protocol and found to produce similar effects of varying magnitudes. In general, the reduced sulfhydryl containing compounds and ascorbic acid were the most effective. Pretreatment with BCNU alone without diamide did not produce this effect. Treatment with BCNU and diamide resulted in a greater than 75% decrease in reduced glutathione levels and a concomitant tenfold increase in glutathione mixed disulfide levels (0.229 vs. 0.030 mumol g-1 wet weight) in the calf trabecular meshwork. The subsequent perfusion with cysteamine reversed this mixed disulfide formation.(ABSTRACT TRUNCATED AT 250 WORDS)

4-Chloromercuribenzenesulfonate↗
PubMed1990

Comparative study of lens proteins of gray squirrel and human.

1. The four crystallins of the gray squirrel lens have been characterized using gel filtration chromatography, polyacrylamide gel electrophoresis, and immunoblotting. Alpha, beta-heavy, beta-light, and gamma crystallins of squirrel lenses have been identified immunologically, and they cross-react strongly with rabbit polyclonal antibodies. The gamma-24 crystallin of the squirrel lens also reacts strongly with monoclonal anti-human lens gamma-24, as shown by its inhibition of the ELISA reaction by 85%. 2. The water-insoluble urea soluble proteins represent non-covalently associated species of soluble crystallins and the lens cytoskeletal proteins. The membrane intrinsic protein in the urea insoluble pellet has a mol. wt of 27,000 but other lower and higher mol. wt components are also present, which were removed by washing with 0.1 NaOH. The N-terminal 30 amino acid of squirrel lens gamma crystallin was found to be identical to that of the bovine (and human) lens. 3. Measurements of the distribution and state of SH and SS compounds in the squirrel lens have shown greater similarities to those of primates than those of rodents. The findings show that on the basis of both protein and sulfur chemistry the squirrel lens is a representative model for studies of oxidative lens changes in diurnal animals, including man.

Adult↗
PubMed1989

Reversal of galactosemic-induced inhibition of PGH synthase activity in cultured lens epithelial cells.

Inhibition of prostaglandin synthesis as determined by prostaglandin endoperoxide synthetase (PGH synthase) activity is associated with polyol accumulation in cultured bovine lens epithelial cells (BLECs) incubated six days in minimal essential medium (MEM) containing 40 mM galactose (Gal). In order to better understand the nature of the correlation between hypergalactosemic exposure, polyol accumulation and inhibition of prostaglandin synthesis, a series of culture media reversal and sorbinil (an aldose reductase inhibitor) addition studies were carried out. BLECs were incubated in Gal for six days, then changed to galactose-free MEM +/- sorbinil for a three day recovery period. PGH synthase activity reduced to 66% of control after six days of exposure to Gal. The simultaneous administration of sorbinil during a nine day Gal incubation significantly protected the enzymatic activity, while the activity of PGH synthase further declined to 41% of control under the same conditions in the absence of sorbinil. Within 72 hours of media reversal, PGH synthase activity equaled or exceeded control values in BLECs switched to either MEM or MEM + sorbinil. Indeed, an enhanced prostaglandin biosynthetic capacity as demonstrated by radioimmunoassay was exhibited with microsomes prepared from cells switched from Gal into Gal-free MEM +/- sorbinil, corroborating the beneficial effect of media reversal. Furthermore, following 72 hours of reversal, the cellular dulcitol level was 93 nmol/micrograms PO4 for BLECs switched to MEM alone; no detectable level of polyol was observed in BLECs changed to MEM + sorbinil. In contrast, the polyol content in BLECs after six days of exposure to Gal was 185 nmol/micrograms PO4 and increased to 334 nmol/micrograms PO4 after nine days of continuous incubation.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1982

Whole blood viscosity in preeclampsia.

Whole blood viscosity was measured in 41 patients with preeclampsia and in 51 normotensive control subjects. The mean viscosity in the preeclamptic group had a highly significant elevation (t = 9.752, p less than 0.001, at a shear rate of 0.1 sec-1 and t = 4.223, p less than 0.001, at a shear rate of 100 sec-1). The slower shear rate gave the better discrimination between the two groups as only four patients with preeclampsia had a value within 1 SD of the mean of the control group. It is suggested that the measurement of whole blood viscosity may be clinically useful in the management of patients with preeclampsia.

Blood Pressure↗
PubMed1982

Long term treatment of two familial hypercholesterolemic heterozygote patients with batch affinity chromatography (BAC).

Two familial hypercholesterolemic heterozygote patients were treated for a period of 2 years using an extracorporeal technique involving batch affinity chromatography. In both cases LDL-cholesterol levels were drastically lowered and HDL-cholesterol levels were normalized. No untoward side effects were noted during the 2 year experimental period. These results confirm the safety and simplicity of this technique to lower considerably LDL-cholesterol levels in whole blood of familial hypercholesterolemic patients who do not adequately respond to conventional medical treatment.

Adult↗
PubMed1980

Removal of cholesterol from blood by affinity binding to heparin-agarose: evaluation on treatment in homozygous familial hypercholesterolemia.

Extracorporeal removal of cholesterol from blood with heparin-agarose conjugates was studied as a means of reducing the plasma cholesterol concentration in two patients with homozygous familial hypercholesterolemia. Both patients were treated in an outpatient clinic. Patient 1 underwent two separate courses of treatment; during the first course of eight treatments, the plasma cholesterol concentration decreased by a maximum of 54% (from 811-370 mg/dl). The six treatments in the second course resulted in a 35% reduction of plasma cholesterol (from 939--632 mg/dl). In patient two, 12 consecutive treatments resulted in a 56% decrease in the concentration of plasma cholesterol (from 768--298 mg/dl). Repetitive treatments in both patients resulted in a new "steady state" with circulating cholesterol levels lower than the pretreatment baseline value. The decrease in plasma cholesterol is mainly due to the removal of low density lipoproteins. The circulating level of high density lipoproteins is unaffected by the treatment. The treatment has no effect on the chemical composition of both low and high density lipoproteins. The extracorporeal treatment of blood with heparin-agarose was well tolerated by both patients and there were no undesirable effects. The effectiveness and simplicity of the present approach makes it attractive as a possible mode of therapy for homozygous familial hypercholesterolemia.

Adolescent↗
PubMedDate not supplied

[Role of nitric oxide in the NKCC2 hyperactivity of Dahl "salt-sensitive" rats].

Renal NaCl reabsorption is increased in Dahl "salt-sensitive" (DS) rats, due to an increased activity of the Na-K-Cl cotransporter NKCC2. On the other hand, nitric oxide (NO) is an inhibitor of NKCC2 and a deficient nitric oxide synthase (NOS) seems to play an important role in salt-sensitivity of DS rats. Here, we investigated the hypothesis that NKCC2 hyperactivity in DS rats is due to a deficient NOS, via the interactions cyclic GMP (cGMP)/cyclic AMP (cAMP) at the level of the thick ascending Henle's loop (TAL). DS rats DS (males, 250-300 g) and their normotensive controls DR ("salt-resistant") are sacrificed, the kidneys removed and NKCC2 activity is measured in medullary TAL (mTAL) as previously described. Medullary contents of NO are measured with a NitroFlux analyser by heat-reduction of nitrates and nitrites to NO. AMPc levels in mTAL are measured by an EIA immunotest. Neither L-NAME (3 mM), nor L-arginine were able to modify NKCC2 activity in mTAL from DS (pre-hypertensive) or DR rats. Levels of NO in the medullary interstitium and AMPc in mTAL were not significantly different between DS and DR rats. Conversely, in DS rats charged with 2% salt (in the food) during 7 weeks, L-arginine significantly inhibited NKCC2 in DS (35.6 +/- 6.8 vs 25.3 +/- 4.9 nmoles/mg protein/min; p<0.05 non-paired Student's t-test), but not in DR rats. In conclusion, NKCC2 in our mTAL preparation of prehypertensive DS and DR rats is insensitive to L-NAME and L-arginine. This suggests the absence of a functional NOS. NKCC2 hyperactivity of prehypertensive DS is therefore not due to a deficient NOS. This was confirmed by the normal levels of interstitial NO and mTAL cAMP in prehypertensive DS rats. Finally, a salt-load seems to induce NOS expression in mTAL of DS rats. This last observation deserves further investigation.

Animals↗