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Biomedical subjects

M Luttges

Publications and source records attributed to M Luttges.

4 recordsLinked to original sources

The BIMDA shuttle flight mission: a low cost microgravity payload.

This paper presents the design, operation and experiment protocol of the Bioserve sponsored flights of the ITA Materials Dispersion Apparatus Payload (BIMDA) flown on the Space Shuttle on STS-37. The BIMDA payload represents a joint effort between ITA (Instrumentation Technology Associates, Inc.) and Bioserve Space Technologies, a NASA Center for the Commercial Development of Space, to investigate the methods and commercial potential of biomedical and fluid science applications in the microgravity environment of space. The BIMDA payload, flown in a Refrigerator/Incubator Module (R/IM) in the Orbiter middeck, consists of three different devices designed to mix fluids in space; four Materials Dispersion Apparatus (MDA) Minilabs developed by ITA, six Cell Syringes, and six Bioprocessing Modules both developed by NASA JSC and Bioserve. The BIMDA design and operation reflect user needs for late access prior to launch (<24 h) and early access after landing (<2 h). The environment for the payload is temperature controlled by the R/IM. The astronaut crew operates the payload and documents its operation. The temperature of the payload is recorded automatically during flight. The flight of the BIMDA payload is the first of two development flights of the MDA on the Space Shuttle. Future commercial flights of ITA's Materials Dispersion Apparatus on the Shuttle will be sponsored by NASA's Office of Commercial Programs and will take place over the next three years. Experiments for the BIMDA payload include research into the following areas: protein crystal growth, thin film membrane casting, collagen formation, fibrin clot formation, seed germination, enzymatic catalysis, zeolite crystallization, studies of mixing effects of lymphocyte functions, and solute diffusion and transport.

Biotechnology↗

Myoelectric paraspinal response to spinal loads: potential for monitoring low back pain.

LBP evaluation has been hampered by the subjectivity of available clinical procedures and the paucity of factual information about spinal dysfunction. Objective indices of spinal pathomechanics would assist the clinician to prescribe and evaluate appropriate treatment. This project assesses paraspinal myoelectric symmetry as a potential objective measure of low back performance. A preliminary group (N = 39) of symptomatic subjects was prospectively tested to choose parameters for the blinded experiment to follow. The experimental group of volunteers (N = 37) were electromyographically monitored while the subjects performed selected postural tasks which did not produce pain. Results were compared to orthopedic tests and history. Four arbitrary myoelectric classifications were defined for subject grouping and comparison: subjects with symmetrical paraspinal task performance, occasional (less than 4 tasks) asymmetry, low amplitude response for quasi-static loads, and large amplitude asymmetry (greater than 4 tasks). Eighty percent of subjects with significant asymmetry of muscle action had recent LBP history. Isolated task testing is inconclusive as a clinical parameter. However, observation of performance of a series of tasks may be useful in clinical diagnosis and monitoring of function in low back pain.

Abdominal Muscles↗

Qualitative and quantitative morphological changes in subcellular fractions from mouse cerebral cortex during postnatal development.

Discontinuous Ficoll-sucrose gradients were used to prepare subcellular fractions from mouse cerebral cortex at various stages of postnatal development. Representative samples of each subcellular fraction were obtained by sedimentation in an analytical ultracentrifuge and each fraction was examined quantitatively and qualitatively by electron microscopy. The amount of synaptosomal material was determined for each fraction on the basis of volume percentage, obtained from a series of contiguous micrographs, to circumvent any sampling error. This allowed an accurate appraisal of synaptosomal distribution during neural development and a direct comparison of the Ficoll-sucrose gradient fractions to the original crude mitochondrial preparations. The distribution of synaptosomal material was shown to be quantitatively altered during neural development, and maturation-dependent changes, at a qualitative level, were described. In addition, the relationship between neural maturation and the relative proportion and distribution of subcellular particles which contain processes tentatively identified as growth cones were characterized.

Animals↗