M M Albala — Page 3 | PubMed Health

PubMedDate not supplied

A simple method for the cryopreservation of human lymphocytes at -80C.

A simple and inexpensive method for the freezing of human blood lymphocytes at -80 C is described. Lymphocytes were frozen in either polystyrene tubes or polyolefin bags. Optimal recovery of cells was obtained when 5% DMSO and 62% plasma were used as cryoprotectants. Viable cell recovery, as assessed by response to phyto hemagglutinin (PHA) and pokeweed mitogen (PWM), was greater than 80% after 60 days of storage. In addition, percentages of T and B lymphocytes were essentially unchanged. The excellent viable cell recovery in polyolefin bags suggests that it is possible to cryopreserve large numbers of lymphocytes in a single container.

Alkenes↗

PubMedDate not supplied

Chronic granulocytic leukemia and carcinoma of the cervix in situ following renal transplantation.

A 26-year-old white female presented with Philadelphia chromosome positive chronic granulocytic leukemia five years after successful renal transplantation and prolonged therapy with azathioprine and prednisone. This patient represents the seventh case of the chronic granulocytic leukemia reported within the population of immunosuppressed renal transplant recipients. Of further interest in our case is the development of carcinoma in situ of the cervix two years following the transplant. Although multiple malignancies have been recognized in renal homografts, the occurrence of chronic granulocytic leukemia and carcinoma in situ in the same patient has not been previously described. A possible mechanism for the development of chronic granulocytic leukemia is the association of chromosome damage by azathioprine and immunoparalysis of the host, thus setting the stage for the emergency of a malignant clone. The importance of close follow-up of patients on prolonged immunosuppressive therapy is emphasized.

Adult↗