Biomedical subjects

M M Livshits

Publications and source records attributed to M M Livshits.

3 recordsLinked to original sources

PubMed1979

[Determination of the concentration of active centers and the antibody heterogeneity index by using the precipitation in fluid reaction].

Equivalence zone in the precipitation test was characterized by the fact that the number of the antigen determinants was equal to the number of active antibodies centres, irrespective of the heterogeneity of the latter. This permits to assess the antibody concentration in the values of the antigen valency. The new method of determination of the antibody heterogeneity index is based on recording the titre of the antigen and antibodies in the equivalence zone.

Animals↗

PubMed1977

[Use of serologic reactions for determining the concentration of the determinants of an antigen and its valence].

By the serological tests with the erythrocytic diagnostic agents it is possible to estimate the concentration of the determinants in the antigenic preparation under study. For this purpose it is necessary to have at one's disposal the data on the molecular weight and concentration of the reference antigen, valence of erythrocytes loaded with the antigen or antibodies, and antibody heterogeneity index. If these values are known, then one can calculate the concentration of antigenic determinants in terms divided by the valence of the reference antigen. With the aid of pure antibodies preparation obtained with the immunosorption procedure it is possible to measure the determinant concentration of the antigen tested and the valence of the reference antigen. For the capsular antigen of plaque bacilli the valence was estimated by two independent methods and in both cases it was close to 10. The valence of the reference capsular antigen permits to calculate the concentration of the antibody molecules in the hyperimmune sera without isolation of pure antibodies.

Animals↗

PubMed1975

[The possibility of predicting the degree of plasmacyte reaction in response to administration of soluble antigen to mice].

The work is devoted to the mathematical description of the accumulation of plasma cells in the spleen of CBA mice immunized intraperitoneally. A dependence between the dynamics of the soluble antigen concentration in the blood and the plasmocytic reaction in the spleen was confirmed under experimental conditions. Index A was suggested for detection of a change from the antigenic function to the accumulation of plasma cells. The mean values of index A were used to compare the calculated values of the plasmocytic reaction and the experimental data; differences between these values were insignificant. Analogously index A was used for predicting the accumulation of plasma cells in the animals to which a mixture of the two soluble antigens (a capsular antigen of the causative agent of plague and a complete antigen of the causative agent of tularemia) was administered. The calculated values of the plasmocytic reaction in this experiment satisfactorily correlated with the data obtained experimentally.

Animals↗