PubMed Health⌕ Search

Biomedical subjects

M M Rogan

Publications and source records attributed to M M Rogan.

5 recordsLinked to original sources
PubMed1994

Enantiomeric separation of salbutamol and related impurities using capillary electrophoresis.

The enantiomeric resolution of salbutamol and its chiral and achiral related impurities is investigated using capillary electrophoresis. The effects of nine varieties of cyclodextrin, cyclodextrin concentration and organic modifier concentration were studied in an attempt to resolve all possible analytes in a complex mixture of salbutamol-related solutes. Eleven components including three enantiomeric pairs were baseline resolved using 112 mM dimethyl-beta-cyclodextrin at pH 2.5 in a citric acid/phosphate buffer. Both methanol and iso-propyl alcohol at up to 20% were found to have a deleterious effect on the separation. Binding constants and mobility values for the free and complexed forms for each solute were determined. The results are interpreted by considering the physical properties of the molecules under the conditions employed and a rationale proposed for the underlying basis for chiral and achiral selectivity.

Albuterol↗
PubMed1994

Quantitative applications and validation of the resolution of enantiomers by capillary electrophoresis.

The separation and quantitation of enantiomers is an important application of capillary electrophoresis. This paper reviews the performance and validation of capillary electrophoretic methods for this application. Limits of detection of < 0.1% are shown for determinations of minor enantiomer levels. Application areas include enantiomeric purity testing of pharmaceuticals and herbicides, reaction rate monitoring, stability testing, and clinical analysis. It is concluded that the number of quantitative application areas will undoubtedly expand as capillary electrophoresis becomes more widely established as an alternative and complement to high-performance liquid chromatography.

Artifacts↗
PubMed1993

Enantioselective enzymatic biotransformation of 2'-deoxy-3'-thiacytidine (BCH 189) monitored by capillary electrophoresis.

Concentrations of the enantiomers of 2'-deoxy-3'-thiacytidine (BCH 189) have been monitored during enantiospecific biotransformation catalyzed by cytidine deaminase. This enzymatic reaction is monitored by capillary electrophoresis (CE) over a 51-h time period. The use of dimethyl-beta-cyclodextrin as a chiral electrolyte additive in a pH 2.3 phosphate buffer system gives enantiomeric separation with results for peak efficiencies, resolution, and analysis time of the order of 25,000 plates, > 1.5 and 30 min, respectively. The features of the CE method are compared with those of the previously favored chiral HPLC method.

Antiviral Agents↗