[Specific and nonspecific immunologic reactivity among children with uveitis].
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Biomedical subjects
Publications and source records attributed to M M Sheĭnbergas.
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Antibody to lymphocytic choriomeningitis virus was determined by the indirect immunofluorescence test in immune sera of guinea pigs and immune ascitic fluids of rats and mice. Among 135 patients with aseptic meningitis serum antibody was found in 11 patients in titers of 1 : 64 to 1 : 128 and in the cerebro-spinal fluid of these patients in considerably lower titers. By the indirect immunofluorescence test antibody in maximum titers was found early after the appearance of meningeal symptoms.
An attempt was made to isolate an infectious virus from FL cells in which arenavirus antigen revealed by immunofluorescence tests persisted. Inhibitors of macromolecular synthesis, ultraviolet irradiation, fusion with BHK 21/13S cells, and transfection of BHK-21/13S cells with DNA from FL cells were tested in order to activate the virus. Negative results were obtained in each of these experiments. Prolonged cultivation of FL cells at 31 degres or 41 degrees C resulted in a two-fold increase in proportion of the antigen-containing cells or its complete elimination, respectively. The likelihood of integration of transcribed proviral fragment into the cell genome is discussed.
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By the indirect immunofluorescence test, antibodies to lymphocytic choriomeningitis virus in titers of 1:32 to 1:512 detected in 12 out of 32 infants under 1 with congenital hydrocephalus. The majority of the mothers of these infants were also shown to possess these antibodies in low and moderate titers. The assumption that lymphocytic choriomeningitis virus infecting the fetus in utero may cause the development of hydrocephalus is substantiated. In 11 of 12 seropositive infants foci of chorioretinal degeneration were found.