Biomedical subjects

M M Sopper

Publications and source records attributed to M M Sopper.

10 recordsLinked to original sources

PubMed2001

Phosphorylation state of the native high-molecular-weight neurofilament subunit protein from cervical spinal cord in sporadic amyotrophic lateral sclerosis.

The intraneuronal aggregation of phosphorylated high-molecular-weight neurofilament protein (NFH) in spinal cord motor neurons is considered to be a key pathological marker of amyotrophic lateral sclerosis (ALS). In order to determine whether this observation is due to the aberrant or hyper-phosphorylation of NFH, we have purified and characterized NFH from the cervical spinal cords of ALS patients and controls. We observed no differences between ALS and normal controls in the physicochemical properties of NFH in Triton X-100 insoluble protein fractions, with respect to migration patterns on 2D-iso electrofocusing (IEF) gels, the rate of Escherichia coli alkaline phosphatase mediated dephosphorylation, or the rate of calpain-mediated proteolysis. The rate of calpain-mediated proteolysis was unaffected by either exhaustive NFH dephosphorylation or by the addition of calmodulin to the reaction. Phosphopeptides and the phosphorylated motifs characterized by liquid chromatography tandem mass spectroscopy (LC/MS/MS) analysis demonstrated that all the phosphorylated residues found in ALS NFH were also found to be phosphorylated in normal human NFH samples. Hence, we have observed no difference in the physicochemical properties of normal and ALS NFH extracted from cervical spinal cords, suggesting that the perikaryal aggregation of highly phosphorylated NF in ALS neurons reflects the aberrant somatotopic localization of normally phosphorylated NFH.

Amino Acid Sequence↗

PubMed1998

Nitration of the low molecular weight neurofilament is equivalent in sporadic amyotrophic lateral sclerosis and control cervical spinal cord.

To determine the extent to which enhanced nitration of the low molecular weight neurofilament subunit protein (NFL) is of pathogenic significance in sporadic ALS, we isolated the neurofilament (NF) from the cervical spinal cord of 15 cases of sporadic ALS and 11 age-matched control cases. Of the three NF subunits, only NFL demonstrated consistent nitrotyrosine immunoreactivity on immunoblots against mouse monoclonal anti-nitrotyrosine antibodies. Regardless of whether the NFL was isolated from the Triton X-100 soluble or insoluble cytoskeletal fractions, the extent of NFL nitration did not differ between ALS and control tissue. Similarly, no differences were observed on either two dimensional isoelectric focusing or NFL peptide maps. These findings suggest that NFL is particularly susceptible to peroxynitrite-mediated nitration in vivo, but reveal no significant qualitative or quantitative modifications in the nitration of NFL isolated from sporadic ALS cervical spinal cord tissue as compared to non-ALS controls.

Adolescent↗

PubMed1997

Enhanced ex vivo cosedimentation of high molecular weight neurofilament protein (NFH) with microtubules following in vivo aluminum chloride exposure: inhibition of dephosphorylation-dependent dissociation.

We have investigated the effect of acute in vivo aluminum exposure on the subsequent ex vivo cross-linking of the high molecular weight neurofilament protein (NFH) with polymerized microtubules. Young adult female New Zealand white rabbits were inoculated intracisternally with 1000 micrograms of AlCl3 in 0.9% NaCl or with 0.9% NaCl alone, and killed 48 hours later. Following isolation of a cytoskeletal-enriched protein fraction from the cervical spinal cord, NFH was purified by either electroelution or column chromatography. Tubulin was isolated from New Zealand white rabbit brains by repeated temperature-dependent polymerization and depolymerization, purified over phosphocellulose, and cosedimented with either phosphorylated or dephosphorylated NFH. Following incubation for 30 minutes at 32 degrees C with tubulin in the presence of 20 microM Taxol, 1.0 mM MgCl2 and 1.0 mM GTP, the insoluble pellet containing NFH/microtubules was isolated. Both the pellet and supernatent were fractionated by SDS.PAGE and the amount of NFH present quantified by transmission densitometry following silver-staining. Results were identical regardless of the technique utilized for the purification of NFH. Control NFH preferentially cosedimented with microtubules when in the fully phosphorylated isoform, but remained in the soluble fraction following dephosphorylation. Phosphorylated NFH derived from AlCl3-inoculated rabbits demonstrated similar binding characteristics to control NFH, but following exhaustive dephosphorylation, exhibited a 4.5 fold induction of NFH/microtubule binding (p = 0.0314). Incubating dephosphorylated control NFH with microtubules in the presence of increasing concentrations of AlCl3 failed to induce similar cosedimentation. These experiments suggest that phosphorylation promotes NFH cross-linking to microtubules. In addition, the phosphorylation/dephosphorylation dependent regulation of NFH cross-linking to microtubules is disrupted following in vivo AlCl3 exposure by a mechanism that s independent of NFH/Al3+ binding.

Aluminum Chloride↗

PubMed1994

Gene complementation using myoblast transfer into fetal muscle.

Gene complementation by myoblast transfer into neonatal or adult muscle has been proposed as a therapy for primary myopathies as well as to augment non-muscle gene products that may be diminished in the adult circulation. This paper describes a technique whereby myoblasts have been injected into limb muscles of normal and dystrophin-deficient (mdx) fetal mice (during the period of active myotube formation and prior to the development of the host's immune competence) without significantly interfering with fetal viability or further maturation. More mosaic myofibers (myofibers containing both host- and donor-derived myonuclei) appear to result from these transfers than have been reported following myoblast transfer into neonatal muscle or adult muscle. The small size of the fetal hosts' muscles and the lack of well-defined connective tissue septa facilitate migration of donor myoblasts into muscle groups distal to the injection site. The use of donor myoblasts derived from a tetraploid variant of a mouse myogenic cell line (MM14) provides a convenient and permanent cytological marker for the recognition of donor myoblasts and donor-derived myonuclei. When MM14 myoblasts are injected into mdx fetuses, whose muscles lack dystrophin, mosaic myofibers contain sufficient dystrophin to be visualized with routine immunohistochemical techniques. The myoblast transfer system, using fetal hosts, described in this study will facilitate the evaluation of myoblasts as vectors to overcome genetic deficiencies that may be manifested during fetal development.

Animals↗

PubMed1993

Modulation of contractile protein gene expression in fetal murine crural muscles: emergence of muscle diversity.

The modulation of contractile protein gene expression in mouse crural muscles (i.e., muscles located in the region between the knee and ankle) during the fetal period (defined as 15 days gestation to birth), resulting in diversity among and within these muscles, has been evaluated with in situ hybridization and correlated with morphogenetic events in the extensor digitorum longus and soleus muscles. During the fetal period extensive secondary myotube formation occurs in the crural muscles, and the myotubes become innervated (Ontell and Kozeka [1984a,b] Am. J. Anat. 171:133-148, 149-161; Ontell et al. [1988a,b] Am. J. Anat. 181:267-278, 181:278-288). At 15 days gestation, hybridization with 35S-labeled antisense cRNA probes demonstrates the accumulation of transcripts for alpha-cardiac and alpha-skeletal actin; MLC 1A, MLC 1F, and MLC 3F; and MHC emb, MHC pn, and MHC beta/slow. At 16 days gestation, accumulation of MHC emb transcripts is reduced (as compared with earlier developmental stages); intensity of signal following hybridization with the probe for alpha-skeletal actin is, for the first time, equal to that for the cardiac isoform; and MLC 1V mRNA accumulation is discernible. At this stage, variation in transcript accumulation for some mRNAs among and within crural muscles becomes evident. Two factors may play a role in the selective distribution of these transcripts: 1) the stage of muscle maturation; and 2) the future myofiber type. At 16 days gestation anterior crural muscles (which mature approximately 2 days before posterior crural muscles; Ontell and Kozeka [1984a,b], ibid., Ontell et al. [1988a,b], ibid.) exhibit a greater accumulation of transcripts for alpha-skeletal actin and for MLC 3F than is found in posterior crural muscles. In muscles that in the neonate are composed, in large part, of slow myofibers, MHC beta/slow and MLC 1V mRNAs accumulate in greater amounts, whereas MHC pn transcripts are less abundant in the soleus muscle than in other crural muscles. By 19 days gestation regionalization of transcript accumulation is more pronounced. The soleus muscle, a predominantly slow twitch muscle in the newborn mouse (Wirtz et al. [1983] J. Anat. 137:109-126) exhibits strong signal after hybridization with probes specific for MHC beta/slow and MLC 1V. While the level of transcript accumulation for the development isoforms, MHC emb, MLC 1A, and alpha-cardiac actin, is greatly reduced in most crural muscles at 19 days gestation, these transcripts persist in the soleus muscle at levels equal ot or exceeding their amount in limb muscles of 13 day gestation mouse embryos.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗

PubMed1993

Contractile protein gene expression in primary myotubes of embryonic mouse hindlimb muscles.

The time course of contractile protein [actin, myosin heavy chain (MHC) and myosin light chain (MLC)] gene expression in the hindlimb muscles of the embryonic mouse (< 15 days gestation) has been correlated with the expression of genes for the myogenic regulatory factors, myogenin and MyoD, and with morphogenetic events. At 14 days gestation, secondary myotubes are not yet present in crural muscles (M. Ontell and K. Kozeka (1984) Am. J. Anat. 171, 133-148; M. Ontell, D. Bourke and D. Hughes (1988) Am. J. Anat. 181, 267-278); therefore, all transcripts for contractile proteins found in these muscles must be produced in primary myotubes. In situ hybridization, with 35S-labeled antisense cRNAs, demonstrates the versatility of primary myotubes in that transcripts for (1) alpha-cardiac and alpha-skeletal actin, (2) MHCembryonic, MHCperinatal and MHC beta/slow, and (3) MLC1A, MLC1F and MLC3F are detectable at 14 days gestation. While the general patterns of early activation of the cardiac genes and early activation of the genes for the developmental isoforms are preserved in both myotomal and limb muscles (D. Sassoon, I. Garner and M. Buckingham (1988) Development 104, 155-164 and G. E. Lyons, M. Ontell, R. Cox, D. Sassoon and M. Buckingham (1990) J. Cell Biol. 111, 1465-1476 for myotomal muscle), there are a number of differences in contractile protein gene expression. For example, in the myotome, when myosin light chain genes are initially transcribed, hybridization signal with probe for MLC1A mRNA is greater than that with probe for MLC1F transcripts, whereas the relative intensity of signal with these same probes is reversed in the hindlimb. The order in which myosin heavy chain genes are activated is also different, with MHCembryonic and MHCperinatal preceding the appearance of MHC beta/slow transcripts in limb muscles, while MHCembryonic and MHC beta/slow appear simultaneously in the myotomes prior to MHCperinatal. In the myotome, an intense hybridization signal for alpha-cardiac and a weak signal for alpha-skeletal actin transcripts are detectable prior to myosin mRNAs, whereas in the limb alpha-cardiac actin transcripts accumulate with myosin transcripts before alpha-skeletal actin mRNA is detectable. These differences indicate that there is no single coordinate pattern of expression of contractile protein genes during initial formation of the muscles of the mouse.(ABSTRACT TRUNCATED AT 400 WORDS)

Actins↗

PubMed1984

Insulin binding in human skeletal muscle.

Insulin binding to crude plasma membranes derived from human skeletal muscle was characterized. Incubations were performed for 22 h at 4 degrees C. Typical insulin binding characteristics were found, i.e., (a) specificity for insulin, (b) pH sensitivity, (c) dissociation of insulin by the addition of excess insulin and (d) concave Scatchard curves. Half-maximal inhibition of 125I-labeled-insulin binding occurred at 1 X 10(-8) M. Affinity constants were 0.76 X 10(9) and 0.02 X 10(9) M-1 for the high- and low-affinity receptor (2-site model), respectively, and the corresponding receptor numbers were 89 and 1450 fmol/mg protein, respectively. The procedures employed permit the determination of insulin binding to small quantities of human muscle (approx. 250 mg).

Adult↗

PubMed1984

Calcium requirements of cardiac myofibril ATPase activity following exhaustive exercise.

Myocardial contractility is reduced in rats following strenuous activity. Thus, the purpose of this study was to determine some of the cellular mechanisms that may contribute to the depressed contractile function. Myofibril ATPase activity was determined with varying free calcium and monomeric vanadate (Vi) concentrations. The Mg2+ stimulated myofibril ATPase activities were significantly reduced in the activity group (E). Myofibril ATPase activity from control animals increased from 0.056 +/- 0.021 to 0.216 +/- 0.030 mumol X Pi X mg-1 X min-1 with 0.1-10.0 microM Ca2+. The addition of 15.0 microM Vi resulted in a 37% decrease in ATPase activity of C animals. With regard to the experimental group, the myofibril ATPase activity at 0.1 and 1.0 microM Ca2+ were depressed (P less than 0.05) with the values at 5.0 and 10.0 microM Ca2+ being similar to the control group (P greater than 0.05). Incubations with Vi resulted in an enhanced myofibril ATPase activity for E compared to C animals. The ATPase activities were increased by 17, 10, 10 and 15% at 3.0, 5.0, 10.0 and 15.0 microM Vi. The results suggest that the exhaustive exercise raises the CA2+ requirement for half-maximal activation of cardiac myofibril ATPase activity and that the contracto-regulatory mechanism of cardiac muscle is similarly altered.

Animals↗

PubMed1983

Effects of menstrual cycle on metabolic responses to exercise.

Selected substrate and hormonal responses to exercise were compared in two phases of the menstrual cycle. Exercise-induced changes in substrate [glucose, lactate, free fatty acids (FFA), glycerol] and hormonal patterns [luteinizing hormone (LH), follicle-stimulating hormone (FSH), insulin, progesterone (P), growth hormone (GH), cortisol] were compared in the follicular and luteal phases of the menstrual cycle in 24-h-fasted (n = 5), glucose-loaded (n = 6; 1.50 g/kg, 20% solution), and control subjects (n = 8). A treadmill walk was maintained for 60 min (30 min, 40% VO2 max; 30 min, 80% VO2 max). Blood samples were obtained 5 min before, 15, 30, 45, and 60 min during, and 30 min after exercise. In the glucose group a blood sample was also taken 20 min before exercise, and glucose was ingested 15 min before exercise. Within each nutritional group the metabolic and endocrine responses to exercise were similar in the two phases for glucose, lactate, glycerol, LH, FSH, and cortisol (P greater than 0.05). In the glucose group the FFA response was lower in the luteal phase (P less than 0.05). In the fasted subjects insulin and GH responses were elevated in the luteal phase (P less than 0.05). P responses in the control and glucose groups were markedly greater in the luteal phase (P less than 0.05). In the fasted subjects no alteration in P occurred in either phase (P less than 0.05), and the LH concentration was lower in these subjects relative to the control groups (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1981

Calcium activation of sarcoplasmic reticulum ATPase following strenuous activity.

The purpose of this study was to examine the effects of varying Ca2+ activated sarcoplasmic reticulum (SR) ATPase activity of fast-twitch (FT) skeletal muscle at exhaustion and during recovery. Wistar rats (200 g) were assigned to control (C), exhausted (E), and three recovery groups (R) at 5, 15, and 30 min. Following exhaustion on a motor-driven treadmill, the gastrocnemius muscles from all groups were excised and frozen. Muscle samples were assayed for ATPase activity in a Ca2+-ethyleneglycol bis (beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA) buffering system. At 1.25 microM Ca2+, a significant depression in Ca2+ activated ATPase activity occurred in the E, 5R, 15R, and 30R groups (1.61 +/- 0.17, 1.87 +/- 0.14, 1.43 +/- 0.29, and 1.62 +/- 0.1 mumol Pi . mg-1 . 10 min-1) compared with C values (2.41 +/- 0.34 mumol Pi . mg-1 . 10 min-1) (p less than or equal to 0.05). At 5.0 microM, Ca2+ activated ATPase activity remained depressed in the E, 5R, and 15R groups compared with C and 30R groups (p less than or equal to 0.05). At 0.75 microM Ca2+, there was no significant difference between groups (p greater than or equal to 0.05). The results suggest that Ca2+ activated SR ATPase activity of fatigued FT muscle may contribute to the decreased force production at exhaustion.

Adenosine Triphosphatases↗