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Biomedical subjects

M Manabe

Publications and source records attributed to M Manabe.

At least 91 records · Page 5Linked to original sources
PubMed1985

Macromelanosomes in X-linked ocular albinism (XLOA).

A case of X-linked ocular albinism is reported. Characteristic Masson-Fontana positive and Dopa positive giant melanin granules were found in keratinocytes, melanocytes and upper dermis. Ultrastructurally the macromelanosome was composed of a dense core and a less dense surrounding mantle.

Adult↗
PubMed1984

Production of blister in normal human skin in vitro by blister fluids from epidermolysis bullosa.

Pathogenic mechanisms involved in the blister formation of epidermolysis bullosa (EB) are not clearly understood at present. In this paper, we attempted to produce experimental blistering in vitro similar to the histologic picture of EB simplex (EBS) and recessive dystrophic EB (RDEB). It was demonstrated by light and electron microscopy that the medium containing fresh blister fluids from the patients with EBS or RDEB could produce similar histologic features in normal human skin (in vitro) to those of the skin lesions of patients (in vivo). This observation may open new avenues of approach to studying these diseases.

Blister↗
PubMed1983

Comparative studies of the marginal band and plasma membrane of the epidermis.

The membranous fraction isolated from stratum corneum by 8M urea-beta ME containing alkaline buffer (pH 9.0) is quite crude when observed by electron microscopy. However, this procedure may be useful for clinical samples, as one can isolate and compare both the soluble (interfilamentous) fraction and the keratin filament from the same sample in addition to the residues (membranous fraction). A further purified membranous fraction was isolated by a new method. Human stratum corneum was chopped and treated with 8M urea-50 mM Tris-HCl (pH 9.0), digested by the use of trypsin, and the product fractionated by a sucrose density gradient to obtain separate single cells without the cytoplasm. One sample was then treated with trypsin for 1 hour and another with urea buffer for 24 hours. Observations revealed a thickened inner membrane (marginal band) of approximately 150A. Each of the membranous samples contained a level of half-cystine markedly higher in amount (around 100/1,000) and involved mostly in the epsilon-(gamma-glutamyl) lysine cross-linkages (around 30%). In order to compare the membranous fraction of horny and living cells (marginal bands and plasma membranes), the fraction was then isolated from living cells. The relative amino acid composition of the membranous fraction of the plasma membrane resembled that of human erythrocytes, but was quite different from that of the marginal band. These comparative studies of biochemical and morphological features suggested the importance of S-S cross-linking enzymes and transglutaminase in the transformation mechanism of the marginal band.

Amino Acids↗