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M Manchester

Publications and source records attributed to M Manchester.

22 records · Page 2Linked to original sources
PubMed1994

The activity of the protease of human immunodeficiency virus type 1 is initiated at the membrane of infected cells before the release of viral proteins and is required for release to occur with maximum efficiency.

The final steps in the production of the type C retroviruses include assembly of the viral core particle and release of virions from the surface of the infected cell. The core proteins are translated as part of one of two precursors, Gag and Gag/Pol, which are cleaved by a virally encoded protease. We examined the interaction between the processing of the human immunodeficiency virus type 1 Gag precursor and the membrane-based assembly and budding of virions. Our results indicate that cleavage by the viral protease is initiated at the membrane of the infected cell during virus release and that protease activity is required for virion release to occur with maximum efficiency.

Autoradiography↗
PubMed1989

Complete mutagenesis of the HIV-1 protease.

Retroviruses encode a protease which needs to be active for the production of infectious virions. A disabling mutation in the protease results in the production of non-infectious virus particles and examination of proteins from these mutant virions reveals unprocessed Gag and Gag-Pol precursor proteins, the substrates of the viral protease. Each amino acid of the HIV-1 protease was individually mutated using a simple mutagenesis procedure which is capable of introducing and identifying missense mutations in each residue of a protein. Phenotypic screening of these mutants in a heterologous assay system reveals three regions within the protease where multiple consecutive amino-acid residues are sensitive to mutation. These results show that random mutagenesis can be used to identify functionally important regions within a protein. Mutants with conditional phenotypes have also been identified within this collection.

Amino Acid Sequence↗