Biomedical subjects
Publications and source records attributed to M Marano.
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Experiments were conducted to evaluate the teratogenic potential of nickel chloride given to developing chick embryos. Nickel chloride was dissolved in saline and injected into chicken eggs at dosages ranging from 0.02 to 0.7 mg per egg. The injections were made into the air sacs of eggs at days 0, 1, 2, 3, and 4 of incubation. Control eggs were injected with an equivalent volume of saline (0.1 ml per egg); in all, 840 chicken eggs were used. All embryos were examined on day eight. The following malformations were observed: exencephaly, everted viscera, short and twisted neck, short and twisted limbs, microphthalmia, hemorrhage, and reduced body size. The dose-response relationship was observed in all of the groups tested. The toxicity and teratogenicity of nickel chloride was the highest in the embryos treated at day 2. The results of the present study indicate that nickel chloride is teratogenic.
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A linear relationship between equieffective concentrations of agonist in the presence and absence of partial agonist was examined statistically. These experimental concentrations permit the estimation of the apparent dissociation constant Kp with a weighted fit. The form of the weights was proposed theoretically and substantiated by simulated experiments. Constants Kp for (+/-)-p-chloroisoproterenol were estimated from the antagonism by three concentrations of (+/-)-p-chloroisoproterenol of the positive chronotropic effects of (-)-isoproterenol on kitten (Kp = 1.75 X 10(-7) M) and guinea-pig (Kp = 1.84 X 10(-7) M) right atria. The Kp values were similar regardless of the concentrations of (+/-)-p-chloroisoproterenol used; they were also similar to a Kp of 1.95 X 10(-7) M) estimated BY Kaumann and Birnbaumer (J. Biol. Chem. 249: 7874-7885, 1974) for the (+/-)-p-chloroisoproterenol-receptor complex coupled to the adenylyl cyclase in cell-free membrane particles of kitten heart. The proposed statistical procedure appears to be useful for systems with sigmoid log concentration-effect curves in which concentrations of the dependent variable are obtained from interpolation.
The objective of this paper was to examine the major anaesthetic problems during transthoracic endoscopic sympathectomy without artificial pneumothorax and to present our experience of 16 cases suffering from Raynaud's disease. For the perioperative management we used a double lumen endo-bronchial tube and balanced anaesthesia (intravenous agents plus isoflurane). Arterial pressure, heart rate, ECG, end-tidal carbon dioxide concentration, SatO2, blood gases and peak inspiratory pressures were monitored. The results showed that no significant changes in these parameters occurred during surgery. Since hypoxaemia is the main problem of the thoracoscopic sympathectomy the A.A. emphasize the necessity to ensure a correct ventilation as well as a haemodynamic stability throughout the procedure. The combination of balanced anaesthesia and double lumen endobronchial intubation seems an advisable method when no artificial pnx is instituted. A close monitoring of the circulatory and respiratory systems is imperative.