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M Marschalkó

Publications and source records attributed to M Marschalkó.

24 records · Page 2Linked to original sources

Serological examinations with TPHA antigen prepared from T. pallidum Budapest strain.

The TPHA test is performed with sheep erythrocytes treated with formalin and tannin solution and conjugated with fragments of pathogenic Treponema pallidum. Absorption media was prepared by adding normal rabbit serum, powdered rabbit testicle, sheep and cow stroma and sonicated Reiter treponeme. The reagents prepared in our laboratory proved to be specific and sensitive enough for routine use. It can be confirmed that the TPHA reaction is a good confirmatory test in the serodiagnosis of syphilis. Further studies are necessary to explain the divergent results between TPHA and TPIT. We plan to carry out detailed investigation. Analysis of patients data in a large population after the problem of storage of our reagents over extended periods has been resolved.

Adolescent↗

Effect of KCN on the motility of Treponema pallidium.

Virulent Treponema pallidum has been shown to survive in KCN-containing artificial medium. Oxygen uptake being sensitive to cyanide, the observation indicates that treponemes do not have a cytochrome oxidase system. KCN had a killing effect only at a 15--30 mM final concentration. The data show that the Budapest strain of T. pallidum is an anaerobic organism.

Animals↗