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Biomedical subjects

M Matĕjka

Publications and source records attributed to M Matĕjka.

At least 19 recordsLinked to original sources
PubMed1984

Isolation of mycobacterial phage from the laboratory strain Mycobacterium leprae murium "Douglas".

The colony microstructure of the laboratory strain Mycobacterium leprae murium "Douglas" cultivated on Ogawa's egg medium was examined. A bioptical sample from the liver of a white mouse subcutaneously infected and observed for ten months was used as inoculum. The inoculum contained 5.2 X 10(9) acidfast rods. The Ogawa's media were incubated in 5% atmosphere of CO2 at 33 degrees C to 37 degrees C for 6 to 10 months. The outgrown colonies were killed with a formol solution, then embedded into the agarparaffin and cut out with the aid of Reichert's microtom. In thin sections there was an apparent vacuolisation of colonies proving the presence of the temperate phage, which was isolated from the bacterial suspension inoculated on the host non lysogenic strain Mycobacterium smegmatis ATCC 607. On the simple agar medium N-4 the number of 2.4 X 10(9) living particles was achieved, which shows the possible use of this phage for differential diagnostic purposes in the taxonomy studies of mycobacteria.

Animals↗
PubMed1982

The microscopical pattern of growth of Mycobacterium leprae-murium "Douglas" in microcolonies.

A thin section technique of Ogawa egg yolk culture medium inoculated with Mycobacterium leprae-murium was found to reveal microscopical growth of the strain which could not be demonstrated by macroscopical examination. A peculiar structure of the growth, characterized by many lytic spots different in size, was observed indicating the possible presence of a temperate phage which may interfere with the synthesis of nucleic acids needed for the active multiplication of M. leprae-murium.

Animals↗
PubMed1981

An electron microscope study of the perfusion-fixed spleen. III. Fine structure of germinal centers in monkey spleens with special reference to the so-called "dendritic reticular cells".

The spleen white pulp of monkey (Macaca mulatta) was studied on electron microscopical level with special reference to cell composition of the light zone of follicular germinal or reactive centers. The presence of a special kind of the so-called "dendritic reticular cells" whose origin appear to have a bearing only on the germinal center formation was confirmed. These non-lymphoid cells, characteristic principally of a well developed Golgi complex and a conspicuous cell coat, overlying mainly the very fine ramified peripheral cytoplasmic processes, seem to support differentiation of small lymphocytes (B-cells). This specialized cell type of peculiar shape and function may be derived most probably from the rest structural reticulum cells under proper circumstances.

Animals↗
PubMed1977

An electron microscope study of the perfusion-fixed spleen. II. Fine structure of the sinus lining cells with respect to filamentous structures in monkey spleens.

In the perfusion-fixed spleen of monkey (Macaca mulatta) were studied with the electron microscope the cytoplasmatic structures of the sinus lining cells. The particular interest find the contractile filaments in this cells, which are important for the cell contraction. The connections to the smooth endoplasmatic reticulum are very strong as in the muscle fiber or muscle cell.

Animals↗
PubMed1977

Potential genetic risks from stationary magnetic field.

Cytogenetic analysis of Dede cell lines (Chinese hamster) was used to study the mutagenic effect of stationary magnetic field (SMF) and the combined effect of SMF and TEPA. Cytogenetic analysis showed a moderate mutagenic effect after exposure to SMF. In combination with TEPA, only the additive effect of the two mutagens was observed.

Animals↗
PubMed1975

Sensitivity to tuberculin and to a nonmammalian sensitin in Mandalay District, Burma.

Delayed hypersensitivity that can be demonstrated with either a strong dose of tuberculin or a conventional dose of a sensitin prepared from certain nonmammalian mycobacteria (mycobacteria of Runyon Groups II and III, e.g., Mycobacterium avium), is known to be highly prevalent in most tropical and many subtropical areas and rare in many temperate zones. Whether such sensitivity interacts significantly with tuberculosis or with leprosy is not known.A study of reactions to tuberculin (PPD-S) and to a sensitin prepared from M. intracellulare (PPD-B) was carried out in villages close to an area in which a clinical trial of the preventive effect of BCG against leprosy was being conducted. The population had not been vaccinated with BCG. Some of the villages were in river valleys that become flooded every year for a long period during the rainy season; others were on slopes above the area subject to floods. The findings showed that sensitivity to the nonmammalian sensitin was prevalent in the area, and thus confirmed previous findings of low-grade tuberculin sensitivity in Burma and neighbouring countries. No difference in this prevalence was found between flooded and nonflooded villages.

Adolescent↗