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Biomedical subjects

M Matoba

Publications and source records attributed to M Matoba.

At least 73 records · Page 4Linked to original sources
PubMed1988

Experimental intraocular lens implantation in the rabbit eye and in the mouse peritoneal space. Part II: Morphological stages of the macrophage on the implanted lens surface.

Macrophages appearing on implanted intraocular lenses (IOLs) in the rabbit eye and in the mouse peritoneal space were observed using Wolter's implant cytology staining and scanning and transmission electron microscopy. In response to the implanted IOL, macrophages in the mouse peritoneal space displayed an activated form with marked ruffles on the surface. They were attached to the IOL with a broad base (sessile macrophage). The macrophages metamorphosed gradually to the flat shape of so-called epithelioid cells with many processes. They showed a tendency to fuse together or become more flat by extending the cytoplasmic lamellipodia and finally disappeared, leaving a thin membrane or a fibrous matrix. Phagocytic activity of macrophages and related cells was also observed. However, those observed on the IOL implanted in the rabbit eye showed less surface structure and appeared to adhere weakly to the IOL.

Animals↗
PubMed1988

Experimental intraocular lens implantation in the rabbit eye and in the mouse peritoneal space. Part III: Giant cell formation on the implanted lens surface.

Among the cell components appearing on an implanted intraocular lens (IOL), the macrophage is the most active participant in the foreign-body response. The morphological stages of the macrophage in the response include not only an activated form and a flat epithelioid cell, but also a giant cell observed in the later stage of the response. The giant cells on the IOLs implanted in the rabbit eye and in the mouse peritoneal space were investigated by means of Wolter's implant cytology staining and scanning and transmission electron microscopic observation. Differences in the two biological environments as the site of IOL implantation are discussed.

Animals↗
PubMed1987

Assay of ptaquiloside, the carcinogenic principle of bracken, Pteridium aquilinum, by mutagenicity testing in Salmonella typhimurium.

The mutagenicity of ptaquiloside, the carcinogenic principle of Pteridium aquilinum, was tested in Salmonella typhimurium TA100 and TA98. Under weakly basic conditions (pH 8.5), ptaquiloside decomposed into a conjugated dienone (considered to be the ultimate form), which was mutagenic in both strains. A novel bioassay, using the pre-incubation method at pH 8.5 with S. typhimurium tester strains was developed for the assay of ptaquiloside extracted from plants. By this bioassay the ptaquiloside content of ferns collected at different localities during various seasons, and in various parts of the plant was determined. The ubiquitous presence of ptaquiloside in fresh plant materials was confirmed. Bracken processed in alkali was found not to contain the carcinogen.

Carcinogens↗
PubMed1987

Efficacy of dibutyryl cyclic AMP in heart failure unresponsive to catecholamines.

The efficacy of dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) was evaluated in eight patients with heart failure unresponsive to catecholamine therapy. Seven patients who were hemodynamically at Forrester's hemodynamic subset stage H-IV and had a left ventricular stroke work index (LVSWI) of less than 20 g-m/m2 despite administration of unloading drugs and catecholamines were studied both hemodynamically and clinically. Another patient with dilated cardiomyopathy, in whom invasive hemodynamic monitoring could not be carried out, was studied clinically. The DBcAMP was administered intravenously at a mean (+/- SD) of 0.05 +/- 0.036 mg/kg/min, and hemodynamic measurements were made 63 +/- 37 min after administration. The cardiac index (CI) increased from 1.92 +/- 0.22 to 2.49 +/- 0.59 L/min/m2, and LVSWI from 14 +/- 4.0 to 18 +/- 5.1 g-m/m2, both significantly (CI, P less than 0.01; LVSWI, P less than 0.025). The total systemic vascular resistance index (TSVRI) decreased significantly from 2,746 +/- 427.2 to 2,218 +/- 582.6 dan.sec.cm-5.m2 ( P less than 0.01). The increase in CI was accompanied by a proportional decrease in TSVRI in all patients. Left ventricular function, which was estimated by the relation between pulmonary arterial end-diastolic pressure and LVSWI, was improved in five of seven patients after administration of DBcAMP. Two patients in whom DBcAMP was given intermittently improved clinically and survived. The authors conclude that DBcAMP has powerful vasodilating and mild positive inotropic effects and hence can be useful for treating heart failure unresponsive to catecholamines.

Adolescent↗
PubMed1985

Scanning electron microscopical observation on the isolated mucosa of rat small intestine: with special reference to the intestinal crypt.

The small intestinal mucosa of rats (male, aged 8 weeks) was isolated by the Bjerknes and Cheng's method (1981): the anesthetized rats were perfused through the left ventricle with a solution of ethylenediaminetetraacetic acid (30 mmol/l) in calcium-magnesium-free Hanks' solution, and then the mucous epithelium was removed from a segment of the duodenum, jejunum and ileum by means of vibration. Three-dimensional images of crypts on the isolated mucosa were obtained by scanning electron microscopy. The length and number of the crypts between the villi were observed to decrease along the intestine from the duodenum to the ileum. The majority of intestinal crypts were simple non-branched tubular glands, however, budding and bifurcation of the crypt ends were often observed. It was relatively rare to find longitudinally split crypts. The shapes of the crypts seems to denote various stages of the proliferation of crypts.

Animals↗
PubMed1984

Ultrastructural localization of thiamine pyrophosphatase activity in the intestinal epithelial cells of adult rats.

Ultrastructural localization of thiamine pyrophosphatase (TPPase) activity was studied in the small and large intestinal epithelial cells of adult rats. In the duodenal and jejunal absorptive cells, TPPase activity was localized on the microvilli, on the lateral cell membrane, and on the lysosome-like bodies. In the ileal epithelial cells, TPPase activity was detected on the lysosome-like bodies, and weak phosphatase activity was observed on the microvilli and on the cisternae of the rough-surfaced endoplasmic reticulum (rER). In the principal cells of the caecum, the ascending colon, and the descending colon, the lysosome-like bodies and the cisternae of the rER showed TPPase activity. Almost all Golgi lamellae, vesicles and GERL elements (NOVIKOFF 1964) in the duodenal absorptive cells were strongly TPPase positive. The 1st to 3rd of the inner Golgi lamellae and the vesicles of the jejunal, ileal, and large intestinal epithelial cells usually showed positive TPPase activity, while the GERL elements were totally negative.

Animals↗