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M Matsuguchi

Publications and source records attributed to M Matsuguchi.

7 recordsLinked to original sources
PubMed1979

Late transcription and simultaneous replication of simian adenovirus 7 DNA as revealed by spreading lytically infected cell cultures.

Miller's technique of spreading DNA was applied to monkey cells productively infected with simian adenovirus 7. This permitted the visualization of cellular DNA transcription, both nucleolar and non-nucleolar, and of late transcription and replication of virus. Virus double-stranded DNA, thin fibres with very few nucleosome-like particles, were observed carrying either transcription or replication complexes. In addition, both RNP transcripts and replication forks were found on some virus duplex DNA. Virus single-stranded DNA replicative intermediates were identified on the basis of their increased thickness and contrast which results from the presence of a DNA binding protein.

Adenoviridae↗
PubMed1979

Microcentrifuge technique and its application for visualizing the simultaneous replication and transcription of an adenovirus DNA.

Miller's technique (microcentrifuge method) of visualizing transcriptional events at the molecular level is introduced with a review on the work that applied this technique, and followed by presentation of an article describing one such application in which the modes of replication and transcription are electronmicroscopically demonstrated in an adenovirus-infected cell nucleus. Adenovirus is chosen owing to its character of multiplying in a mammalian cell nucleus. Recently, there have been many important discoveries on the modes of DNA replication and RNA transcription through biochemical probes. It is confirmed by Miller's technique that large primary precursor messenger RNA is first transcribed at a late stage in a cell nucleus productively infected with adenovirus. Evidence is shown that a replicating viral DNA molecule can be simultaneously transcribed.

Adenoviridae↗
PubMed1978

Purification and the ultrastructure of a bacteriocin produced from Shigella sonnei strain 100052.

A bacteriocin produced by strain 100052 of Shigella sonnei (shigellacin 52) was purified about 80-fold from a mitomycin C-induced culture supernatant. The purified preparation gave a single band on sodium dodecyl sulfate-gel electrophoresis with an approximate molecular weight of 10,000. The negatively stained electron micrograph of the purified bacteriocin preparation revealed the existence of three different particles. They were a small cylindrical tube (4.2 by 6.0 nm), a ring-shaped particle and a filamentous structure. The molecular weight calculated from the shape of the small tube was approximately 50,000. The latter two particles appeared to be constructed from subunits which were morphologically similar to the cylindrical tube. These results suggested that the morphological subunit of shigellacin 52 consisted of five chemical subunits with molecular weights of 10,000 each, and it assembled into two types of polymers, a ring-shaped particle and a filamentous structure, which seemed to be the main components of the purified shigellacin 52 preparation.

Bacteriocins↗