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M Matta

Publications and source records attributed to M Matta.

30 records · Page 2Linked to original sources
PubMed1993

[Evaluation of a new rapid ELISA technique for detecting human cytomegalovirus antibodies (CMV). Applications in emergency blood transfusion and in organ transplantation].

Immunocubes can detect very low HCMV IgG levels with at least the same sensitivity as the reference technique: ELISA. Samples containing bacteria showed no particular interference on the test, and results for non-infected material were in agreement with those of ELISA. The rapid test did not give any false positive reactions even with samples from patients with very high antiherpes immunoglobulins levels. Samples from transplant patients at the beginning of seroconversion or diluted to IgG titers corresponding to ELISA cut-off values were always detected as positive by immunocubes. In view of the rapidity of the test, its high specificity, its ability to detect levels corresponding to extremely low IgG signals in ELISA, and the possibility of conserving objective proof of the test, we conclude that this rapid immunocube technique is of great interest for current serologic screening in an emergency context, especially for transfusion and organ transplantation.

Antibodies, Bacterial↗
PubMed1993

[Alternative strategy for the diagnosis of HIV infection in sub-saharan Africa. Value of the sequential combination of the ELISA test and a 2nd generation rapid test].

The "conventional" algorithm for HIV testing based on the confirmation of all positive anti-HIV screening reactions by Western blot requires sufficient laboratory facilities and is expensive, that limits its use in developing countries, such as in subsaharian Africa. The apparition of second and third generation screening ELISA which are very sensitive and specific, as well as the development of rapid tests which are simple, visually read, and sufficiently sensitive and specific, has permitted the design of "alternative" strategy for HIV testing utilizing the association of 2 ELISA and/or rapid tests, in order to limit the use of a confirmatory assay. Alternative strategies are less expensive, yield generally very high sensitivity and specificity, and have proved to be valuable for African countries. In this study, 5 alternative strategies, using different associations of two second generation screening tests, one classical ELISA (Genelavia mixt) and one rapid test (Test PACK HIV-1/HIV-2 AB) have been retrospectively evaluated in the field in Bangui, Central African Republic, with a panel of 130 sera (prevalence of HIV infection: 42.7%). The strategy using two sequential screening tests (Test Pack HIV-1/HIV-2 AB following by Genelavia mixt) with the confirmation of discordant results by Western blot permitted to diagnose HIV-1 infection in Bangui with a sensitivity, a specificity and a positive predictive value of 100%, and to reduce the cost of more than 50% in comparison with the conventional strategy. Such an alternative strategy could be useful for the individual notification of HIV serology in Bangui.

Adult↗
PubMed1990

Lichen planopilaris: a clinicopathologic study.

Three clinicopathologic variants of lichen planopilaris are described. The first is characterized clinically by individual keratotic follicular papules and histologically by a lichenoid inflammatory cell infiltrate confined to the follicular epithelium. The second variant consists of erythematous to violaceous plaques, some of which show follicular prominence; the histologic appearance is that of a lichenoid inflammatory cell infiltrate that affects both follicular and interfollicular areas. The third variant manifests as follicular papules of the scalp with concomitant or subsequent cicatricial alopecia. In this variant the histologic hallmark is a lichenoid, follicular and interfollicular inflammation, associated with or followed by scarring. Overlap among the three variants exists, and hence the concept of a disease spectrum ranging from pure follicular involvement without evidence of clinical scarring to cicatricial alopecia of the scalp is advocated.

Adult↗
PubMedDate not supplied

Free and decomplexed human immunodeficiency virus p24 antigenaemia in black patients with AIDS and AIDS-related disorders.

OBJECTIVES: To determine the clinical signification of free and decomplexed p24 antigenaemia in HIV1-infected black individuals. PATIENTS AND METHODS: Sixty African patients with pre-AIDS (n = 12) and AIDS (n = 48), hospitalized in the Centre National Hospitalier Universitaire de Bangui, Central African Republic, were prospectively included. 15 HIV1-seronegative healthy African subjects served as controls. Serum p24 antigenaemia was detected using a polyclonal and a monoclonal sandwich enzyme immunoassay (EIA). All sera were tested again after acid pretreatment (45 mn incubation of serum with a 0.5 N solution of HCl at +37 degrees C). RESULTS: The polyclonal EIA and the monoclonal EIA detected respectively four (7%) to six (10%) free p24 antigen positive samples from the HIV1-infected individuals, with a similar frequency in pre-AIDS and AIDS patients. None of the controls were found p24 antigen positive. After acid pretreatment, the rate of detectable p24 antigenaemia increased significantly, leading with the monoclonal EIA to a 47% positivity rate (p < 0.001), and the patients with AIDS became more frequently p24 antigen positive (54%), than the patients with pre-AIDS (17%) (p = 0.02). CONCLUSION: Detectable p24 antigenaemia in HIV1-infected African subjects could be obtained in nearly half of cases by increasing the sensitivity of the assay using monoclonal EIA and acid hydrolysis of circulating immune complexes. Decomplexed p24 antigenaemia in HIV1-infected Black African patients could have predictive signification similar to free p24 antigenaemia in Caucasians.

AIDS-Related Complex↗