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Biomedical subjects

M Matumoto

Publications and source records attributed to M Matumoto.

At least 19 recordsLinked to original sources
PubMed1992

Double antibody sandwich ELISA for the detection of rubella virus antigen.

We developed enzyme linked immunosorbent assay (ELISA) for the detection of rubella virus antigen, using two monoclonal antibodies, MC-7 and MC-22. The double antibody sandwich ELISA method was carefully standardized and found to be sensitive enough to detect as small as 2.5 ng protein of rubella virus. The infective titers by the double antibody sandwich ELISA closely related to those judged by interference of vesicular stomatitis virus in RK-13 cells. The method is simple, sensitive, and readily applicable to the detection of rubella virus.

Animals↗
PubMed1991

Immunosuppressive effect of infectious bursal disease virus strains of variable virulence for chickens.

Infection of chicks with attenuated Lp and Sp clones of the RF-1 strain of infectious bursal disease virus was shown to exert no immunosuppressive effect, whereas the parent strain RF-1tc and the original virulent strain RF-1wt were immunosuppressive. One-day-old chicks infected with Lp and Sp clones showed no suppression of immunological response to live Newcastle disease vaccines B1 and TCND, and to bivalent infectious coryza vaccine. On the other hand, infection with RF-1tc or RF-1wt strains was immunosuppressive for these vaccines. The immunosuppressive effect of RF-1tc and RF-1wt strains was more pronounced for infectious coryza vaccine and B1 vaccine than for TCND vaccine. The immunosuppressive effect of RF-1tc and RF-1wt strains was lower when chicks were infected with these strains at the age of 21 days than when they were infected at one day of age.

Animals↗
PubMed1991

Persistent hematomas in Japanese black cattle with impaired platelet aggregation function and large granule eosinophils.

In Japanese black cattle with large and long-existing hematomas, platelets was impaired in collagen aggregation function in vitro. There was no statistically significant difference from control animals in the tests of PT (prothrombin time) and PTT (partial thromboplastin time) for extrinsic and intrinsic blood coagulation system. Aside from impaired collagen aggregation function, platelets in the hematoma cattle showed the similar aggregation patterns as the normal cattle, when ADP, serotonin (5-HT), thrombin, arachidonic acid, epinephrine and ristocetin were used as agents for inducing aggregation. Decreased aggregation function as well as impaired collagen-induced release response in platelets suggested the hematoma cattle to be of storage pool disease (SPD). The impaired platelet was postulated to be a main cause of the large and long-existing hematomas. All of the hematoma cattle with impaired platelet functions had the eosinophils in peripheral blood of which granules were fewer and larger than normal ones. These large eosinophil granules were peroxidase positive and periodic acid Schiff (PAS) staining negative as typical eosinophil granules.

Adenosine Diphosphate↗
PubMed1991

Participation of H1-receptors in histamine-induced contraction and relaxation of horse coronary artery in vitro.

The mechanisms of histamine-induced contraction and relaxation were investigated in rings isolated from a middle part of the left descending coronary arteries of horses. Intact and endothelium-denuded preparations were compared. Rings of horse coronary arteries contracted in response to histamine in a concentration dependent manner, but some of them relaxed with lower concentrations and contracted with higher concentrations. Removal of the endothelium abolished the relaxation and potentiated the contraction. The pD2 values were 4.70 +/- 0.08 in the rings with intact endothelium and 4.95 +/- 0.08 in endothelium-denuded rings. Histamine-induced contractions in intact and denuded preparations were not affected by an H2-antagonist, cimetidine, but were inhibited by an H1-antagonist, diphenhydramine in non-competitive manner in the rings with endothelium and in competitive manner in denuded rings. After precontraction with PGF2 alpha or norepinephrine, histamine relaxed preparations with intact endothelium (pD2 value, 7.80 +/- 0.11), although histamine-induced relaxations were not observed in denuded preparations. The relaxation was competitively inhibited by diphenhydramine. Relaxing response was significantly attenuated by methylene blue, quinacrine, L-nitro-arginine, gossypol and AA861 but not by indomethacin. These results suggest that the histamine-induced contraction and relaxation in horse coronary arteries are mediated mainly by H1-receptors in the smooth muscle and endothelium, respectively, and H1-receptor activation of endothelial cells may liberate vasodilator substances.

Animals↗
PubMed1991

[Effect of UFT by oral administration using a murine hepatic metastasis model].

We examined the antitumor effect of UFT, a 5-FU derivative using a hepatic metastasis model in mice. BALB/c and CDF1 mice were given orally UFT (30 mg/kg, 50 mg/kg daily or 120 mg/kg every other day) for 20 days after inoculation of colon-26 tumor cells into the portal vein. The number of metastatic nodules and the weight of the liver decreased significantly in all groups of UFT treatment. The therapeutic effect with 50 mg/kg UFT was better than those with the other doses. NK and LAK activities of spleen cells obtained from UFT-treated tumor bearing mice were not significantly different from the untreated control. IL-1 and IL-2 production of the spleen cells were also not significantly changed by the treatment with UFT. These results suggested that the oral administration of UFT represents an antitumor effect against hepatic metastasis in mice without sever suppression of the host immune system.

Administration, Oral↗
PubMed1990

Production of circulation interferon in children with varicella.

Interferon (IFN) alpha was detected in the serum of children with varicella. The serum IFN was demonstrated in the acute phase of varicella and became negative 7 days after the onset of rash. Serum IFN was detected during the incubation period in two patients tested before the onset of rash. Some correlation was shown between the positive rate of circulating IFN and the severity of illness.

Acute Disease↗
PubMed1977

Polyadenylate in the virion RNA of mouse hepatitis virus.

Mouse hepatitis (MH) virus was grown in SR-CDF1-DBT, a mouse cell line, and purified by ammonium sulfate precipitation and by density gradient centrifugation. Extraction of RNA from purified virions with 1% SDS and sedimentation analysis of the RNA revealed a major 50S component and two minor components. Treatment of virions with phenol/chloroform also produced the 50S component, although its yield was lower. MH virion RNA can bind to a poly(U)-fiberglass filter, indicating that MH virion RNA contains poly(A). A poly(A)-like fragment was isolated by digestion with ribonuclease A [EC 3.1.4.22] and T1 [EC 3.1.4.8] and by DEAE-Sephadex column chromatography. Analysis of the fragment for base composition showed it to be an adenine-rich material. Its chain length was about 90 nucleotides, as determined by ion-exchange chromatography and gel electrophoresis.

Cell Line↗
PubMed1977

[Semi-excitation in nerve and electric nerve model (ENM) (author's transl)].

The "semi-excitation" is an excitation (like phenomenon) found by the authors, first in ENM and then in nerve during observation of the action potential and impedance decrease associated with excitation. The phenomenon was further investigated in relation to the action potential and impedance decrease (for AC of 13 kHz) of the stimulated site on the sciatic nerve and ENM. The nature of the phenomenon was the following. 1. The configuration of the action potential and impedance decrease were almost the same as that of the (complete) excitation, but the durations of the potential change (plateau) and impedance decrease depended on entirely to the duration of stimulating current. 2. Submaximum amplitude of action potential and impedance decrease were graded by the intensity of the stimulating current, but the threshold of this excitation was found. 3. During or after the semi-excitation, absolute or relative refractory period was not found. 4. Both in nerve and ENM, transition from complete excitation to semi-excitation or vice versa was observed. 5. Semi-excitation was found to occurr in the state of reduced membrane potential of the nerve and ENM.

Action Potentials↗
PubMed1976

Epizootic congenital arthrogryposis-hydranencephaly syndrome in cattle: isolation of Akabane virus from affected fetuses.

Previous serolgoical studies strongly suggested Akabane virus to be the etiologic agent of epizootic abortion and congenital arthrogryposis-hydranencephaly in cattle, and this view was further corroborated in this study by the isolation of the virus from an aborted fetus in an epizootic of the disease and from a fetus extracted froma cow which was suggested by serologic tests to have a recent infection with the virus. The latter fetus had histological changes of encephalomyelitis and polymyositis, and specific antigens of Akabane virus was shown by the immunofluorescent technique in brain tissues as well as skeletal muscular tissues. The virus was recovered from various fetal tissues and fluids, and in relatively large amounts from brain, spinal cord, cerebral fluid, skeletal muscles and fetal placenta. The intracranial inoculation of suckling mice, 1-2 days of age, was the most sensitive system for Akabane virus isolation and HmLu-1, a continuous cell line from hamster lung, seemed almost as sensitive as suckling mice.

Abortion, Veterinary↗
PubMed1976

Mouse hepatitis virus (MHV-2). Plaque assay and propagation in mouse cell line DBT cells.

Various factor sinfluencing the plaque formation of mouse hepatitis virus (MHV-2) in DBT cell monolayers were studied and a practical method for plaque assay was developed. Infected DBT cells yielded high-titered virus and were a satisfactory source of complement-fixing viral antigen. The predominant cytopathic effect of MHV-2 in DBT cells was cell rounding and detachment, but no syncytial formation was observed. Fluorescent antibody staining revealed specific fluorescence only in the cytoplasm of infected DBT cells. In one-step growth experiment, newly formed virus was first recognized within 4-hr postinfection and showed subsequently a rapid exponential increase. Release of newly formed virus from the cell was rapid, and a continuous release lasted for a certain period of time. The average per-cell yield of active virus was estimated to be about 6-7 X 10(2) plaque-forming units.

Animals↗