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M McClellan

Publications and source records attributed to M McClellan.

6 recordsLinked to original sources

Appropriateness of care. A comparison of global and outcome methods to set standards.

The RAND-UCLA Health Services Utilization Study previously analyzed the appropriateness of use of carotid endarterectomy based on a literature review and global expert judgments. In this study, for 45 of the same clinical indications used in the RAND-UCLA Study, the authors compare the appropriateness judgments based on the global judgment method to appropriateness ratings based on probability estimates of specific outcomes that were provided by the same panel of experts. The authors asked these experts to estimate, for each clinical indication, the likelihood of important medical outcomes (i.e. stroke within a year) in the presence and absence of endarterectomy. Using decision analysis, the appropriateness of endarterectomy for these 45 indications was then calculated. For only two of the eight physicians were the Spearman rank-order correlations between these two methods of judging appropriateness significant and positive. (Correlations for the eight physicians ranged from 0.45 to -0.38). This result was produced by: 1) the tendency of the experts to estimate relatively poor outcomes for seriously ill patients whether or not endarterectomy was performed; and 2) a far less consistent effect of clinical factors on outcome estimates than on global judgments. Better methods for incorporating probability estimates into a global rating process must be developed. The authors found excellent agreement between the panelists' relative outcome estimates for common endarterectomy indications and the observed stroke rate for these same indications, suggesting that one promising method is to use specific outcome data to "anchor" expert judgments.

Aged

Inverse diffusion methods for data peak separation.

Previous methods for separation of overlapping data peaks include geometrical assessment and Fourier deconvolution. On the basis of inverse diffusion theory, we present new separation methods suitable for convenient programming and rapid calculation of Gaussian area contributions. Both continuum and discrete inverse diffusion models are described. Example computations are given for biological data: density gradient centrifugation, isoelectric focusing electrophoresis, and high-pressure liquid chromatography.

Centrifugation, Density Gradient

Immunocytochemical localization of estrogen receptors in the macaque endometrium during the luteal-follicular transition.

We examined changes in estrogen receptors (ERs) in endometrial stromal and epithelial cells in cynomolgus macaques during artificially induced menstruation and repair. We used Silastic implants filled with either estradiol (E2) or progesterone (P) to treat spayed animals for 14 days with E2 followed by 14 days of E2 plus P. We then withdrew the P (but not the E2) implants and removed uteri 0, 0.5, 1, 2, 3, 4, 5, 7, and 14 days later. Uterine tissues were assayed biochemically for ER content, fixed for histology and frozen for immunocytochemistry of ER with monoclonal antiestrophilins. On day 0, ER levels in endometrium were low [1330 +/- 201 (n = 9) fmol/mg DNA]. An increase in total receptor was evident by 3-4 days of P withdrawal 2762 +/- 190 (n = 6) fmol/mg DNA; P less than 0.001]. Total receptor concentrations increased linearly with time from 0.5-7 days of P withdrawal (r = 0.88). On day 0, staining for nuclear ER in the glandular epithelium and stroma of zones I, II, and III of the endometrium was negative. Beginning at 12-24 h and continuing through 4 days of P withdrawal, nuclear staining became detectable and increased in intensity only in endometrial stromal fibroblasts and myometrial smooth muscle cells. The glandular epithelium of the endometrium did not develop nuclear staining until 5-7 days of P withdrawal, coincident with a 10-fold increase in the mitotic index of the epithelium of the upper zones. Thus, the increase in endometrial ER levels that occurred during the first 5 days of an induced luteal-follicular transition took place almost exclusively in stromal fibroblasts.

Animals