Urokinase versus heparin in acute myocardial infarction: no difference?
Explore the source record for details and available documents.
Drug Therapy, Combination↗
Biomedical subjects
M McConnell
Publications and source records attributed to M McConnell.
Restriction endonuclease and genetic mapping studies indicate that the vegetative genome of the temperate, Salmonella-specific bacteriophage, epsilon 15, is circularly-permuted.
Data from physical and genetic mapping studies show that the vegetative genome of the temperate, Group E 1 Salmonella bacteriophage, epsilon 15, is circularly-permuted. Preliminary evidence suggests that the circular permutation of the epsilon 15 genome is non-random.
DNA Restriction Enzymes↗
Developmental regulation of Drosophila DNA topoisomerase II.
Affinity-purified polyclonal antibodies were used to quantitate steady-state levels of DNA topoisomerase II (topo II) throughout Drosophila development. Although wide fluctuations were recorded at different stages, these fluctuations were paralleled by changes in levels of the nuclear lamin, a nuclear structural protein used as an internal standard. The exception to this was adult males where lamin levels were significantly elevated relative to topo II. Northern blot analyses of topo II and lamin mRNA, performed in conjunction with immunoblot analyses of protein revealed fluctuations in levels of the two different messages that paralleled changes in each other and in their respective translation products. Biochemical and immunochemical analyses were complemented by indirect immunofluorescence and immunoperoxidase experiments performed in situ. topo II was found distributed throughout nuclei in most but not all cell types examined. These results for Drosophila topo II are apparently at odds with those obtained by others working in vertebrate systems (see, for example, Heck, M. M. S. and W. C. Earnshaw. 1986. J. Cell Biol. 103:2569-2581; Heck, M. M. S., W. N. Hittelman, and W. C. Earnshaw. 1988. Proc. Natl. Acad. Sci. USA. 85:1086-1090) and suggest that in Drosophila, topo II may not be a useful marker for the proliferative state.
Animals↗
Studies on the initial interactions of bacteriophage epsilon15 with its host cell, Salmonella anatum.
Explore the source record for details and available documents.
Adsorption↗
Variation in the structure and bacteriophage-inactivating capacity of Salmonella anatum lipopolysaccharide as a function of growth temperature.
Growth temperature affects both the structure and the phage-inactivating capacity of Salmonella anatum A1 lipopolysaccharide. Whereas S. anatum cells normally synthesize smooth lipopolysaccharide when grown at physiological temperature (37 degrees C), a partial smooth-rough transition occurs when cells are grown at low temperature (20 to 25 degrees C). The synthesis at low growth temperature of lipopolysaccharide molecules lacking O-antigen was detected both by increased sensitivity of cells to the rough-specific bacteriophage Felix O-1 and by fractionation of oligosaccharides derived from lipopolysaccharide by mild acid hydrolysis. Growth temperature-induced changes in the structure of S. anatum A1 lipopolysaccharide also affected its ability to inactivate epsilon15, a bacteriophage that binds initially to the O-antigen portion of the molecule. Purified lipopolysaccharide prepared from cells grown at low growth temperature exhibited a higher in vitro phage-inactivating capacity than did lipopolysaccharide prepared from cells grown at physiological temperature (37 degrees C).
Chemical Phenomena↗
An approach to preoperative visits.
Explore the source record for details and available documents.
Nurse-Patient Relations↗
An anaerobic technique for increasing bacteriophage plaque size.
Explore the source record for details and available documents.
Anaerobiosis↗
Regulation of Membrane Flexibility in Human Erythrocytes.
We have used spin-labels to detect prostaglandin E induced changes in erythrocyte membranes. The observed changes in spin-label resonance spectra can be mimicked in erythrocyte ghosts by loading them with cAMP or cGMP. These changes can also be observed by adding either of these cyclic nucleotides to intact cells. This entry of cyclic nucleotides into intact cells is blocked by an inhibitor of the anion channel. We suggest that the observed changes in paramagnetic resonance spectra are due to changes in lipid "fluidity" that are brought about by changes in the biochemical state of membrane-associated proteins (such as spectrin) and in the direct or indirect biophysical interactions of these proteins with membrane lipids.
Benzenesulfonates↗
Fusion of dipalmitoylphosphatidylcholine vesicle membranes induced by concanavalin A.
The temperature dependence of fatty acid spin label resonance spectra and freeze fracture micrographs of sonicated dipalmitoylphosphatidylcholine vesicles in the absence and presence of concanavalin A demonstrate a strong interaction of concanavalin A with these lipid membranes, which results in fusion of the vesicles. The rate of this reaction as followed with use of magnetic resonance exhibits a pronounced maximum at 36 degrees, the midpoint of the phase transition range of dipalmitoylphosphatidylcholine vesicles. This maximum is discussed in terms of structural fluctuations, which are maximal in the phase transition range of the membranes.
Binding Sites↗