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Biomedical subjects

M McCormack

Publications and source records attributed to M McCormack.

At least 55 records · Page 3Linked to original sources
PubMed1982

Solid-phase radioimmunoassay of cell specific chromosomal proteins.

A solid-phase radioimmunoassay has been developed for the detection of cell specific chromosomal proteins which require the presence of DNA for their immunoreactivity. The assay involves the binding of chromatin particles sonicated under highly controlled conditions to PVC plates followed by first and second antibody with extensive detergent washing. The sensitivity and specificity of the assay system compares favourably with the complement fixation technique.

Binding Sites, Antibody↗
PubMed1982

Antenatal diagnosis of sickle cell disease: amniotic fluid cell DNA analysis.

Using recombinant DNA methods and amniocentesis, the antenatal diagnosis of sickle cell disorders can be accomplished. This article presents the authors' experiences with 21 pregnancies at risk using HpaI and Hind III restriction enzyme digestions of adult leukocytic and fetal amniotic fluid cell DNA. The authors were able to establish direct beta A- and beta S-globin gene linkages to the restricted fragments in 9 families. In 8 families no direct linkages could be determined and estimates of exclusion of fetal beta S homozygosity were used. These estimates were made on the basis of the frequencies of association of the HpaI fragments with the beta S and beta A genes in the Afro-American population. In 4 families, no estimates or linkages could be established, and the patients were counseled in reference to fetoscopy. The data also indicated the frequencies of association of the HpaI fragments with the beta A and beta S gene in the New Jersey-New York population. These studies did not seem to indicate any exclusive preferential segregation of the HpaI and Hind III polymorphisms.

Amniotic Fluid↗
PubMed1979

Impairments in limb actions after dorsal funiculi section in cats.

A study of the forces exerted by cats against the ground when jumping from a platform equipped with force transducers, was carried out. The study, complemented by film analysis of the jump, attempted to disclose some of the mechanisms underlying the decrease in efficiency and accuracy in releasing a moving piece of liver observed after dorsal funiculi section above C1 level. The results showed that after the lesion, peak force and height of jump were significantly reduced. However, angle of take-off, an index of force distribution in the three axes of rectangular coordinates remained unchanged. Intact cats consistently extend their forelimbs in a smooth and progressive way toward the liver in order to release it. The distance between the tips of the forelimbs remained more or less constant during the flight. Postoperatively, instead of the regular pattern described, the extension of the limbs was interrupted by fast marked flexion movements and the distance between their tips increased significantly during the flight. Peak extension of the forelimbs was also decreased after surgery. The results are interpreted in terms of the different disruption of the various components (proprio and exteroceptive) that make up fasciculus gracilis and cuneatus of the dorsal funiculi.

Animals↗
PubMed1976

A hypothesis for the increased oxygen affinity in haemoglobin Malmö.

Haemoglobin Malmöbeta97His leads to Gln, a high oxygen affinity haemoglobin which causes secondary erythrocytosis, is transmitted in an autosomal dominant manner. A hypothesis accounting for the high oxygen affinity, hyperbolic oxyhaemoglobin dissociation curve, and the relatively normal Bohr effect is presented. The purified abnormal haemoglobin from the present family provided biochemical and functional data for this hypothesis based on the allosteric model proposed by Perutz. Experimental results support the formation of a chemical bond between the -SH proton of the beta93 cysteine and the amide of oxygen of the substituted beta97 glutamine as an explanation for the high oxygen affinity of haemoglobin Malmö.

Hemoglobinopathies↗
PubMed1976

Diagnostic potential of lysosomal hydrolases in body cavity effusions.

Hexosaminidase, alpha-mannosidase, beta-galactosidase, beta-glucuronidase, and arylsulphatase A were measured inperitoneal and pleural effusions from patients with benign, malignant, and inflammatory disorders. Compared with the benign transudates, all enzyme activities were moderately elevated in malignant effusions and markedly elevated in inflammatory effusions. The assay of hexosaminidase and and alpha-mannosidase indicated clearly the underlying pathology in most specimens studied. This method could be of clinical value when the cause of an effusion is in doubt, particularly since the diagnostic criteria are independent of the presence or absence of tumour cells in the aspirate.

Arylsulfatases↗
PubMed1975

Bilateral fibrosarcoma of the epididymis.

The tenth case of primary fibrosarcoma of the epididymis is reported. Contrary to most previous cases, excision biopsy was followed by a lengthy tumour-free period, and a unique feature was bilateral epididymal recurrence.

Adolescent↗