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M McKeag

Publications and source records attributed to M McKeag.

3 recordsLinked to original sources
PubMed1981

The rapid determination of erythrocyte porphyrins using reversed-phase high performance liquid chromatography.

The extraction, separation and quantification of free acid erythrocyte porphyrins are described. The porphyrins are extracted from whole blood using 3:1 ethyl acetate/acetic acid (v/v) and separated using the reversed-phase mode of high performance liquid chromatography (RPLC). The compounds are detected on-line spectrofluorometrically using an excitation wavelength of 404 nm and an emission cut-off filter of 550 nm. Excellent resolution of the porphyrin free acids is achieved in less than 6 min. The analytical recoveries for protoporphyrin IX and zinc protoporphyrin IX were greater than 90% with relative standard deviations for day-to-day analysis under 6%.

Acetates↗
PubMed1977

Temperature control system for water-perfused suits.

A system used to control skin temperature in human subjects wearing water-perfused garments is described, It supplies 8 l/min at 10 psi with water temperature controlled within +/-0.1 degree C. Temperature control is facilitated by a low circulating thermal mass and a fast responding heater based on a commercially available quartz heat lamp. The system is open so that hot or cold water can be added from the building mains to produce rates of change or water temperature exceeding 5 degrees C/min. These capabilities allow semiautomatic control of skin temperature within +/-1 degree C of desired wave forms. Potential hazards and associated safety devices are described.

Humans↗