A system for immuno and nucleic transfers.
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Autoanalysis↗
Biomedical subjects
M McKoon
Publications and source records attributed to M McKoon.
Etched surfaces of plastic embedded and frozen hydrated gastrointestinal tissue.
Scanning electron microscopy (SEM) is most often used to describe the structure of natural surfaces, but it can also be used to examine surfaces created artificially by sectioning or cryofracture. When plasma etched resin sections are studied, the SEM combines some of the functions of a light microscope and a transmission electron microscope. When etched frozen hydrated tissue is examined the groundwork is laid for the use of X-ray microanalysis for the study of elemental concentrations in tissue unexposed to chemical agents of any kind. Examples are given of the study of jejunum and stomach with these two techniques. The results are integrated with data from conventional SEM images to produce composite diagrams which assist with the interpretation of function and topographical morphology.
Animals↗
Postnatal synaptic development of the rat.
Presynaptic boutons of the dorsomedial region of the nucleus tractus solitarius (NTS) were studied during the first 30 days of postnatal development of the rat. Electron micrographs were analyzed by conventional and stereological techniques to determine the area and volumetric density and fraction, and the number per linear length on both the dendrites and cell bodies. The numerical density and volumetric fraction of boutons in the NTS increased rapidly within the first 10 postnatal days. The number of boutons per area and volume remained stable between 10 and 30 days. Twice as many boutons synapsed on the larger dendrites than the cell bodies during the first 10 days, and then increased to 4 times the number of synapses per linear length on the cell body after the tenth postnatal day. The rapid synaptic development and shift in shift in sites of synaptic contact on neurons of the NTS suggest synaptic contacts rapidly change during early postnatal development.
Animals↗
Low temperature scanning electron microscope studies of mouse small intestine.
Etched frozen hydrated specimens of mouse small intestine have been examined with low temperature scanning electron microscopy as a preliminary to X-ray microanalysis. Recognizable images have been obtained of most of the known histological features of the gut. Nuclear and cytoplasmic details were often seen. Ice crystal damage was evident, although the degree of artefact depended on the cell type being examined and also varied from cell to cell or within cells. The same specimens were later examined with resin light microscopy and transmission electron microscopy. These two techniques confirmed that preservation was adequate for identification of cells and tissues, although cavities were seen, representing ice crystal damage. These preliminary results indicate that SEM of etched, frozen, hydrated specimens provides adequate identification of cellular detail to allow further work using X-ray microanalysis to be carried out.
Animals↗