DNA isolation from chloroform/methanol-treated mycobacterial cells without lysozyme and proteinase K.
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Chloroform↗
Biomedical subjects
M Mestdagh
Publications and source records attributed to M Mestdagh.
Correlation of pncA sequence with pyrazinamide resistance level in BACTEC for 21 mycobacterium tuberculosis clinical isolates.
Mutations in the pncA gene, encoding pyrazinamidase, are considered the major mechanism of pyrazinamide (PZA) resistance in Mycobacterium tuberculosis, but resistant strains containing the wild-type gene have been described. The correlation of pncA sequence with PZA resistance level was examined for 21 M. tuberculosis clinical isolates. Susceptibility patterns were determined for 100, 300, and 900 microg/ml concentrations of the drug in BACTEC. Insertions and deletions and a substitution in the putative promoter region led to high-level resistance, whereas substitutions within the open reading frame seemed to confer variable levels of resistance. Variable resistance levels and PZase activities were also observed among isolates lacking pncA mutations. The high-level resistance (900 microg/ml) in pncA wild-type isolates highlights the clinical significance of these isolates. These data also suggest that there may still be more than one alternative mechanism leading to PZA resistance in M. tuberculosis isolates.
Amidohydrolases↗
Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis.
Sixty-two Mycobacterium tuberculosis isolates were tested for pyrazinamidase activity, and their pyrazinamide susceptibility was determined by the radiometric method. Sequencing of pncA genes in the 23 resistant strains revealed mutations in 16 pyrazinamidase-negative strains, 11 of which had not been previously described. Six isolates containing wild-type pncA might possess alternative resistance mechanisms.
Amidohydrolases↗
Scleromyxedema: case for therapeutic suggestion.
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Aged↗
Elastoïdosis with cysts and comedones (Favre and Racouchot).
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Aged↗
[Glucagonoma and an associated dermatological syndrome].
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Adenoma, Islet Cell↗
In vivo effects of five secretin analogs on fluid and potassium secretion from the rat pancreas.
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Amino Acid Sequence↗
The pancreatic secretion of zinc in man and rat.
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Adult↗