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M Michaud

Publications and source records attributed to M Michaud.

85 records · Page 5Linked to original sources
PubMed1981

Test systems for detection of human T associated Ig-related surface determinants using chicken antiglobulin reagents.

In earlier experiments the mixed antiglobulin rosetting reaction (MARR) and the direct antiglobulin rosetting reaction (DARR) were found to be of similar sensitivity in demonstration of immunoglobulin (Ig)-bearing B lymphocytes. These test systems were used in the present study together with chicken anti-F(ab')2, anti-IgM and anti-IgA antibodies to detect Ig-related determinants on human peripheral blood T cells. Although for each antiglobulin reagent the reactivity of viable T cells in the MARR and the DARR appeared to be optimal after treatment of the lymphocytes with neuraminidase, the MARR was always more sensitive than the DARR. Further, when test conditions were established so as to avoid erroneous detection of Ig bound to T cell Fc receptors, the MARR was found to be more sensitive than indirect immunofluorescence. Under the most sensitive mixed antiglobulin rosetting conditions, the mean number of T cells expressing F(ab')2-, alpha- and Mu-related determinants was 99%, 50% and 39% respectively. The complete inhibition of the MARR by human IgM when anti-IgM antibodies are used and the more than 70% inhibition by IgA when anti-IgA antibodies are used, largely exclude false positive rosette formation due to contaminating specificities directed against non-Ig determinants on T cells. Trypsin treatment of T cells removed all determinants recognized by anti-IgM and anti-IgA antibodies and these cells reexpressed these determinants during in vitro culture; this indicates that these determinants are T cell products and do not represent adsorbed Ig molecules.

Animals↗
PubMed1977

Dentin dysplasia, type II: a rare autosomal dominant disorder.

Dentin dysplasia, Type II, is a rare autosomal dominant disorder. The primary teeth are amber and translucent and the pulp chambers are obliterated. The permanent teeth have a normal to brown-gray coloration and a thistle-tube pulp configuration with multiple true denticles. To date, only five families with this disorder have been reported. This article presents two additional families. Light and scanning electron microscopy of an affected primary incisor showed the dentin, including the mantle layer, to be highly disorganized throughout. Possible pathogenic events associated with the phenotype are discussed.

Adolescent↗
PubMed1977

Oral manifestations of acute leukemia in children.

Seventy-seven children with acute leukemia were examined. A wide spectrum of oral findings was observed as the consequence of direct leukemic infiltration of tissue, infection resulting from bone marrow and immunosuppression, impaired healing responses, and poor oral hygiene. Guidelines for dental treatment are provided.

Adolescent↗
PubMedDate not supplied

Control of the expression of thrombospondin 1 in human breast cancer cell lines.

Thrombospondins (TSP) are adhesive glycoproteins which are synthesized, secreted and incorporated into the extracellular matrix of a variety of cells, including mammary epithelial cells. These molecules are included in a gene family that is composed of at least four members. Their roles in the mammary gland are associated with two contradictory functions: antiangiogenesis and tumor invasiveness. To define the role of TSP1 in the mammary gland, the steady-state level of TSP1 mRNA in both normal (HB100) and breast cancer cell lines (MCF-7, T47-D, MDA-MB-231, ZR-75-1) was established. We also observed that the TSP1 protein synthesis is down regulated in breast cancer cell lines as compared to the HB100 cell line. These results show that TSP1 is expressed in well differentiate cells, and suggest that TSP1 could be associated with the antiangiogenesis.

Breast↗