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Biomedical subjects

M Miegeville

Publications and source records attributed to M Miegeville.

At least 19 recordsLinked to original sources
PubMed1992

Microsporum gypseum complex in man and animals.

Twenty-eight strains of the Microsporum gypseum complex isolated from humans and animals were studied. The perfect form was found for 25 of the isolates. Nannizzia incurvata was the species most frequently involved in human pathology, while Nannizzia gypsea was most frequently found on animal lesions. Nannizzia fulva was rarely involved pathologically and Nannizzia corniculata was not isolated during this study. It is surprising to note that this species was not found even though most of our strains (22/28) came from Africa. Reliable methods are not available for differentiating among the anamorphs, which are commonly called M. gypseum, Microsporum fulvum or Microsporum boullardii. The Sabouraud medium conventionally used for medical mycology makes almost no distinction among them. We found that the species could be easily distinguished by colonial and microscopic features when grown on Takashio medium. When strains are atypical, sexual reproduction remains the reference technique but, in most cases, Takashio medium makes it possible to avoid this long drawn-out procedure.

Adolescent↗
PubMed1989

The role of dental canal fillings in the development of Aspergillus sinusitis. A report of 85 cases.

Radiopaque concretions in the maxillary sinus in cases of sinusitis are often observed in infections with aspergillosis. For several authors, such features are considered to be typical of these infections. For us this foreign body in most cases is believed to be related to overfilling of the teeth. We have previously drawn attention to this fact. We report 85 cases of aspergillosis of the maxillary sinus. Cases involving immunosuppressed patients were excluded because of very different clinical conditions. A radiopaque foreign body was seen in 94% of the cases. Of this group, 85% were believed to be related to overfilling of maxillary teeth with dental paste, particularly since evidence for endodontic treatment was found in the premolar/molar region. An image of intrasinus dental paste was demonstrated in 12% of the cases as a direct extension of filling paste from affected teeth. The nature of the dental paste is important because the zinc contained can stimulate the growth of Aspergillus fumigatus. In vitro studies in our laboratory also showed that the growth of A. fumigatus was stimulated with a low concentration of zinc.

Adolescent↗
PubMed1983

[Use of scanning electron microscopy coupled with transmission electron microscopy in comparative studies of the relation between Schistosoma mansoni and Salmonella typhimurium].

Relations between Schistosoma mansoni and Salmonella typhimurium are studied in vivo and in vitro using scanning and transmission electron microscopy as complementary methods. Salmonellae adhesion is a specific process materialized in special places of male and mature schistosome tegumental surface. Interactions are marked by bacterial strong fibres creating a network all around Schistosoma where Salmonellae are dividing. Membrane junction is the last stage leading to symbiotic balance between two biologic systems.

Animals↗
PubMed1983

[Babesiasis, pediatric malaria: does confusion exist in Africa?].

The authors describe a case of febrile disease acquired by a nursling in Africa: was it induced by Pl. falciparum or by Babesia sp.? In spite of numerous microscopic and serological appraisals from veterinary and medical authorities, it is difficult to state definitively this subject, but it is more probably a babesiosis of canine or rodent origin.

Animals↗
PubMed1983

[Specificity in the relationship between Salmonella typhimurium and Schistosoma mansoni].

Special interaction between Salmonella typhimurium (STM) and Schistosoma mansoni is considered under two complementary aspects, in vivo and in vitro, using scanning and transmission electron microscopy. The resulting observations have obviously permitted us to discover a large specificity in these adhesion phenomena that seems to lead to the fusion of membranes between the two organisms. Microanalysis trials executed in these areas of strong affinity were attempted.

Animals↗
PubMed1981

[Possible transfer between sarcomatous mouse cells (BP8) and yeasts (Saccharomyces cerevisiae, whole bodies and protoplasts)].

From our observation in vitro, we can suggest that : a direct contact between sarcomatous cells and physiologically active yeasts appears to be necessary for the transfer of the radioactive labelling; the presence of a filter with pores of 1.2 micrometer diameter prevents passage of fragments of DNA or RNA between the cells; this transfer is a very small fact, but implies about the third of the yeasts.

Animals↗
PubMed1978

[Observation of Schistosoma mansoni by scanning electron microscopy].

The integumental surface of adult Schistosoma mansoni was studied by scanning electron microscopy (SEM) at 220 to 10,000 magnifications. SEM shows certain basic features such as spines in the oral sucker and the acetabulum which may facilitate rasping and attachment of the parasite to stay in the bloodstream of the definitive host. It seems likely that SEM visualization will be a means for differentiation some species of the genus Schistosoma.

Animals↗
PubMed1977

[New scanning electron microscopy contribution to the study of yeast protoplasts].

Study by scanning electron microscopy enables us to describe with precision the morphology of protoplasts. We can confirm that the wrinkles more or less perceptible on the protoplasts studied, do belong to the morphology of those just mentioned, that the presence or the absence of globulous component is connected with the physiological activity of the cell and that it is possible to distinguish the different protoplasts thanks to their morphological aspect.

Candida↗
PubMed1977

[Autoradiography of the exchanges that can occur between murine sarcoma cells (BP 8) and yeast cells (Saccharomyces cerevisiae, complete or protoplast)].

We can tell after observing the resulting negative that yeasts are able, in our experimental conditions, to collect a sequence of ADN and of ARN proceeding from the cancerous cells. Then, those yeasts could turn their synthesis towards the production of new materials. If those informed yeasts were introduced into a mouse, they would induce at the level of the immunocompetent cells a specific immunizing antitumoral power.

Animals↗
PubMedDate not supplied

[Deviation of the life cycle of Dipetalonema viteae (Filarioidea)].

Dipetalonema viteae is a filarial that can evoluate among hosts zoologically broadly apart (Ixodides and Argasides), but always gathered from meriones burrows. Its evolution is, on the contrary, blocked among most of the other ticks, particularly among Ornithodoros erraticus morphologically very similar to the normal vector. Our work concerns the experimental deviation of the cycle of Dipetalonema viteae, with its possible adaptability in the bosom of an intermediate of fowl tropism and a permanent host, different from a gnawing.

Animals↗
PubMedDate not supplied

[Release of "Saccharomyces cerevisiae" protoplasts: scanning electron microscope study (author's transl)].

In a previous study we described the minimal methodology used to obtain protoplasts from ascomycetous yeasts. Using a reducing agent associated with 1,3-beta-glucanase at 26 degrees C, protoplasts were invariably obtained. In the present study we localized the disruption spots of the cell wall using the two same reagents. The observations were made with the scanning electron microscope. The disruption site was always in the subterminal region, and this very simple structure (proteins with disulphide bridges and 1,3-beta-glucans) was opposite the birth-scar. The dissociation of the two reagents showed that a small part of the yeast population was able to release protoplasts with only glucanase. We believe these very sensitive yeasts (2 to 10% population) to be very young cells. These disruption sites seemed very different from budding-sites. They might be identical with elongation-sites, or with the opening in the ascus-wall during germinating ascospore release.

Glucan Endo-1,3-beta-D-Glucosidase↗