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M Misu

Publications and source records attributed to M Misu.

10 recordsLinked to original sources
PubMed1982

Vasoactive intestinal polypeptide (VIP) immunoreactivity of endocrine-like cells in the feline pyloric mucosa.

Immunoreactivity to VIP by endocrine-like cells in the feline pyloric mucosa was examined by using three kinds of region-specific anti-porcine VIP sera. VIP-immunoreactive endocrine-like cells were detected clearly with all of the VIP antisera used. They were located mainly around the neck of the pyloric glands. Some of these endocrine-like cells showed dilution-dependent immunoreactivity against VIP antisera. The immunostaining intensity of VIP-immunoreactive endocrine-like cells showing dilution-independence could not be distinguished from those of nerve elements. The present results suggest that the immunoreactivity with properties very similar to those of authentic VIP may be present in the endocrine-like cells of the feline pyloric glands.

Animals↗
PubMed1981

Intranuclear filamentous inclusions in the gastro-entero-pancreatic (GEP) endocrine cells of birds.

Intranuclear filamentous inclusions were found in the normal endocrine cells of the avian stomach and pancreas. These inclusions were composed of a bundle of closely packed filaments (6-8 nm in diameter), being ultrastructurally similar to those found in the nucleus of various neurons. Most of them appeared as single rod- or spindle-shaped bodies; aggregations of two or more inclusions were rarely seen within a single nucleus. Cells with an intranuclear inclusion often contained a cytoplasmic fibrillar bundle similar to the intranuclear inclusion.

Animals↗
PubMed1981

Segi's cap, a large aggregation of endocrine cells on the intestinal villi, occurs also in cattle and pig fetuses.

Segi's cap, a huge aggregation of basal-granulated cells on the top of the intestinal villi has been known only in human fetuses. This study reveals that the peculiar structure occurs also in cattle and pig fetuses. Segi's cap in these animals mainly consists of argyrophil endocrine cells and, in the cattle fetus, includes gastrin-, somatostatin-, motilin- and secretin-immunoreactive cells.

Animals↗
PubMed1980

Ultrastructure of avian gastrin cell granules.

Gastrin cells in the pigeon, quail, gull and kite were identified at ultrastructural level by immunocytochemistry, using the consecutive semithin/ultrathin section technique. In contrast to the gastrin granules known in mammals, the avian gastrin granules generally contained a consistent dense core accompanied by a clear halo between the core and limiting membrane. The mean diameters of gastrin granules in the pigeon, quail, gull and kite were 211 +/- 37, 247 +/- 45, 331 +/- 61 and 353 +/- 73 nm respectively. The carnivorous birds seemed to possess larger gastrin granules than the grain-eating birds. The species differences in the size and fine structures of the gastrin granules were discussed.

Animals↗
PubMed1979

Distribution and frequency of gastrin cells in the digestive tract of the Japanese quail.

The gastrin cells of the Japanese quail were studied histologically and immunocytochemically. Cells reacting with antiserum to gastrin (gastrin cells) were demonstrated by the peroxidase-labelled antibody method and showed brownish cytoplasm. They also were stained argyrophil by the Grimelius' silver method. Gastrin cells were found in the epithelium of the pyloric region and small intestine and not in any other regions. They were the most numerous in the pyloric region (382.14 +/- 12.77/1.25mm2), next in the ileum (3.79 +/- 1.24/1.25mm2) and duodenum (2.93 +/- 0.62/1.25mm2), and the least in the jejunum (0.93 +/- 0.62/1.25mm2). Remarkable concentration of gastrin cells in the pyloric region has thus been demonstrated.

Animals↗
PubMed1978

Endocrine cells in the pyloric region of the Japanese quail (Coturnix coturnix japonica).

The endocrine cells of the pyloric region of the Japanese quail have been studied by light and electron microscopy. The quail pyloric region is a narrow circular zone (about 2 mm in width) between the gizzard and the duodenum. The endocrine cells are concentrated in this region, and they are represented by argyrophil cells. At least five types of endocrine cells are identified in this region by electron microscopy. Type I cells are characterized by having round and large granules (350--550 nm in diameter) whose contents are high to moderate in electron density. Type II cells are characterized by their round and medium-sized granules (250--350 nm) which possess a dense core and a clear halo. Type III cells are characterized by the presence of round and small-cored granules (150--250 nm). Type IV cells contain round and large granules (350--550 nm) of high to low electron density. Type V cells are identified by the occurrence of microfilaments and polymorphous granules of high electron density. All five cell types are recognized as open type cells. The type I, II and III cells are located mainly in the lower half portion of pyloric glands, and the other cell types are located only in the upper half portion of the glands. The type I and II cells are found much more frequently than other cell types. The relationships between the endocrine cells in the quail pyloric region and those of the mammalian digestive tracts were discussed.

Animals↗