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Biomedical subjects

M Mitsunaga

Publications and source records attributed to M Mitsunaga.

18 recordsLinked to original sources
PubMed1989

[A case report of Swyer-James syndrome].

A 43-year-old man with Swyer-James syndrome (SJS) was reported. The left side of his chest radiograph and computed tomogram was hyperlucent with decreased vascular markings. Pulmonary angiogram and scintigram showed markedly decreased blood flow of the left lung. Statistical study of the patients with SJS in Japan was done.

Adult↗
PubMed1988

[A clinicopathologic study of primary malignant lymphoma of the thyroid with a report of six cases].

From 1977 to 1984, 500 patients with thyroid disease (426 females and 74 males) were seen at the Kure Kyosai Hospital. Among them 6 patients (1 female and 5 males; all older than 50 y) were found to have a primary malignant lymphoma of the thyroid. Histologically, two were identified as being SNC, two were D-Mx, one was a D-L, and the last an IBL (plasmacytoid), according to the Working Formulation. Three patients manifesting a high grade malignancy died within 6 months after the diagnosis. Thyroid autoantibodies were detected in four patients, and, histologically, three of the four had chronic thyroiditis. The relationship between primary malignant lymphoma of the thyroid and chronic thyroiditis is discussed.

Aged↗
PubMed1987

Cytophilic anti-thyroglobulin antibody and antibody-dependent macrophage-mediated cytotoxicity in Hashimoto's thyroiditis.

Antibody-dependent macrophage-mediated cytotoxicity was studied to determine the significance of cytophilic anti-thyroglobulin antibody (ATgA) present in the sera of patients with Hashimoto's thyroiditis. Effector cells were normal human monocytes or guinea-pig peritoneal exudate cells, and target cells were human thyroglobulin(Tg)-coated chicken erythrocytes. Cytotoxicity was evaluated by morphological observation and by 51Cr-releasing assay. Normal human monocytes rapidly destroyed ATgA-bound Tg-coated chicken erythrocytes by extracellular cytolysis and by phagocytosis. On the contrary, human monocytes "armed" with cytophilic ATgA destroyed Tg-coated chicken erythrocytes slowly and to a lesser extent, and only by extracellular cytolysis. When normal monocytes or peritoneal exudate cells were incubated with Tg-coated chicken erythrocytes in the presence of the sera of patients with Hashimoto's thyroiditis, phagocytosis occurred rapidly, but extracellular cytolysis developed rather slowly. These data suggest the possibility that human monocytes participate in antibody-dependent cell-mediated cytotoxicity (ADCC) in vivo, which may be an important destructive mechanism in Hashimoto's thyroiditis. It is also possible that ATgA cytophilic for monocytes render non-immune peripheral monocytes cytotoxic against Tg-bearing cells.

Antibody-Dependent Cell Cytotoxicity↗
PubMed1982

Determination of prednisone and prednisolone in human serum by high-performance liquid chromatography--especially on impaired conversion of corticosteroids in patients with chronic liver disease.

A reliable and rapid method is described for the determination of prednisone and prednisolone in human serum by high-performance liquid chromatography, using a Zorbax-SIL column with dichloromethane-ethanol (92.5:7.5) as eluent, with UV detection at 254 nm. Metabolites and endogenous hydrocortisone did not interfere with the determination of prednisone and prednisolone. The alteration of corticosteroid concentrations in serum from patients with chronic liver diseases was studied following a single oral administration of prednisone or prednisolone (30 mg). The proposed method showed good separation of several corticosteroids and was time-saving, suitable and reliable for the routine analysis of corticosteroids in human serum.

Chromatography, High Pressure Liquid↗
PubMed1980

Cytophilic antithyroglobulin antibody and antibody-dependent monocyte-mediated cytotoxicity in Hashimoto's thyroiditis.

Antithyroglobulin (anti-Tg) antibodies cytophilic for human monocytes were detected in the serum of 30 of 45 patients with Hashimoto's thyroiditis using the passive rosette technique. These antibodies conferred on normal monocytes the ability to form rosettes with Tg-coated erythrocyres (E-Tg) in vitro. The percentage of E-Tg rosette-forming monocytes was correlated with serum anti-Tg antibody titers measured by tanned sheep red cell hemagglutination. Most serum cytophilic activities were recovered in the immunoglobulin G fraction and were not affected by heating to 56 C for 30 min or ultracentrifugation at 105,000 X g for 60 min. Passive E-Tg rosette formation by monocytes was immunologically specific and was inhibited by the addition of small amounts of free Tg into the medium but was not inhibited by the addition of normal human serum. The anti-Tg antibody-armed monocytes became cytotoxic against Tg-coated chicken erythrocytes and lysed target erythrocytes by an extracellular mechanism. It was suggested that monocytes might be armed by cytophilic antibodies in vivo, since monocytes of patients with Hashimoto's thyroiditis showed increased E-Tg binding (rosette formation) relative to monocytes from control subjects. These findings support the possible pathogenetic involvement of monocytes in human autoimmune thyroiditis.

Animals↗
PubMed1979

Recurrent transient thyrotoxicosis with painless thyroiditis--a case report.

A case of a 40-year-old woman who was suffering from painless thyroiditis with recurrent transient thyrotoxicosis is reported. Acute exacerbations occurred four times during the past ten years, two after delivery and two after catching a cold. Serum thyroid hormones increased, though radioiodine uptake by the thyroid was very low and no inflammatory signs were observed. The histological findings of the thyroid were of atypical thyroiditis and not consistent with either chronic lymphocytic thyroiditis or subacute thyroiditis. Tanned sheep red cell hemagglutination titers for anti-thyroglobulin antibodies (TRC) and for anti-microsomal antibodies (MHA) were negative or low. The disease seems to be rare and the pathophysiology and etiology are discussed.

Adult↗
PubMed1977

[Detection of the antithyroglobulin antibody precipitated with polyethylene glycol (PEG) (author's transl)].

It is well known that the antithyroglobulin (anti-Tg) antibody plays an important role in the pathogenesis of autoimmune thyroiditis (chronic thyroiditis). The anti-Tg antibody is detected by double diffusion in agar gel, the fluorescent antibody technique and the tanned red cell haemagglutination test (TRC). The most widely used method is the TRC test, but it is negative in about 30 percent of the patients with chronic thyroiditis. In this paper, we have reported the detection of the anti-Tg antibody in serum using a modified Farr's method. In our method, PEG was used instead of ammonium sulfate to precipitate the immune complex formed in vitro between labelled Tg and the autoantibody. Percent 125I-Tg precipitated was 4.7 +/- 3.1 percent in normal controls; 20.4 +/- 11.4 percent in TRC negative sera were detected by this method. A good correlation was found between TRC titer and percent 125I-Tg precipitated by the PEG method in patients with chronic thyroiditis. By this method, the anti-Tg antibody was also detected in the sera of rabbits immunised with human Tg earlier than that detected by the TRC and double diffusion tests. The sensitivity and simplicity of this method provide a useful tool in detecting the anti-Tg antibody in clinical as well as in experimental work.

Animals↗