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Biomedical subjects

M Mlyncek

Publications and source records attributed to M Mlyncek.

At least 19 recordsLinked to original sources
PubMed2005

Surgical management of endometrial cancer in Slovak Republic.

AIM: To evaluate the routine surgical treatment of endometrial cancer in Slovak Republic in relation to current international recommendations. METHODS: A retrospective study based on a questionnaire was undertaken. Data on surgical and post-operative adjuvant therapy of endometrial cancer patients in Slovakia in 2001 were collected, assessed and validated for good clinical practice. RESULTS: We presented data of 298 cases of endometrial adenocarcinoma from 48 of 66 Slovak gynecologic departments. Laparotomy with hysterectomy and bilateral salpingo-oophorectomy was performed in 280/298 patients. Peritoneal washings were examined in 41/298 cases. Lymphadenectomy (pelvic and/or para-aortic) was performed in 52/298 of the women. Malignancy remained undiagnosed in 29/298 of the cases until it was detected by histological investigation of extirpated uterus. CONCLUSION: The study demonstrates that surgical management of endometrial cancer is far from optimal. It seems necessary to restrict treatment to the centres with gynecologic oncologists trained in pelvic surgery.

Adenocarcinoma↗
PubMed2005

Leptin directly controls secretory activity of human ovarian granulosa cells: possible inter-relationship with the IGF/IGFBP system.

AIMS: The aim of our in vitro studies was to understand the role of leptin and the insulin-like growth factor I/insulin-like growth factor protein (IGF/IGFBP) system in controlling human ovarian function. METHODS: We studied the action of leptin (0, 1, 10, or 100 ng/ml) and immunoneutralization of IGF-I using specific antiserum (0.1%) on the release of progesterone (P), estradiol (E), oxytocin (OT), IGF-I, IGFBP-3, and prostaglandins F (PGF) by these cells using radioimmunoassay/immunoradiometric assay. RESULTS: It was found that leptin stimulated the secretion of OT, IGFBP-3, and PGF. It suppressed the secretion of E and IGF-I, but not P, into the medium. The addition of antiserum against IGF-I decreased IGF-I output, increased P, OT, IGFBP-3, and PGF secretion, and had no effect on E release. Immunoneutralization of IGF-I also prevented or reversed the effects of leptin on P, E, IGF-I, IGFBP-3, PGF, but not on OT. CONCLUSIONS: These observations (1) demonstrate that leptin directly controls the secretory activity of human ovarian cells, (2) confirm the involvement of IGF-I in the regulation of ovarian cells, and (3) suggest an inter-relationship between leptin and the IGF/IGFBP system in the control of these functions and the involvement of IGF/IGFBP system in mediating leptin action on the ovary.

Cells, Cultured↗
PubMed2005

Peritoneal cytology in endometrial cancer.

The results as well as prognostic interpretation of peritoneal washing cytology in endometrial cancer cases is still controversial. The incidence rate of positive cytology varies widely and the clinical significance of the positive results, especially in patients with early stage of endometrial cancer remains also controversial. Prognostic significance of malignant peritoneal cytology in endometrial cancer patients in comparison with those with ovarian cancer has been less well defined. At present, positive peritoneal cytology is not a negative prognostic factor itself, but it enhance other negative prognostic indicators. Literature regarding the significance of peritoneal cytology in endometrial cancer was reviewed in order to draw conclusions for possible therapeutic implications in patients with positive cytologic findings.

Endometrial Neoplasms↗
PubMed2003

[Sacral colpopexy using prolene mesh].

OBJECTIVE: To present two case reports of the surgical correction of vaginal vault prolapse with prolene mesh. DESIGN: Case Report. SETTING: Department of Gynecology and Obstetrics, Hospital Nitra. METHOD: Description of surgical method. CONCLUSION: Sacral colpopexy is method of the choice for correction of vaginal vault prolapse in younger sexually active women.

Female↗
PubMed2002

[Supplemental imaging methods in evaluation of the extent of cervical carcinoma].

OBJECTIVE: To present a review of literature and to analyse of contribution of imaging methods in staging of cervical carcinoma. SUBJECT: Review. SETTING: Department of Obstetrics and Gynaecology, Medical Faculty Trnava, Slovak Republic. SUBJECT AND METHOD: Discussion about relevant evidence from literature, information database (MEDLINE) review, and results from own prospective study. CONCLUSION: FIGO staging of cervical carcinoma is based on clinical examinations but is complemented by imaging methods to optimalize treatment planning. From used imaging methods (computed tomography, magnetic resonance imaging, lymphography, sentinel node mapping, ultrasonography) is combination of abdominal and endoluminal ultrasonography the best available and affordable method with significant benefit in decision making procedure.

Endosonography↗
PubMed2002

[Acebutolol in the treatment of arterial hypertension in pregnancy--comparison with commonly used hypertensive agents].

OBJECTIVE: The main purpose of our study was to analyse efficacy and safety of acebutolol (Sectral) in the treatment of hypertension in pregnancy. DESIGN: Cohort clinical study comparing efficacy and safety of acebutolol with other antihypertensive drugs by 77 patients and their 81 newborns. The number of 48 patients were treated by acebutolol. SETTING: Internal Medicine and Clinical Pharmacology Department, Regional Hospital, Nitra and The Faculty of Health Service and Social Work, University of Trnava; Department of Gynaecology and Obstetrics, Regional Hospital Nitra and The Faculty of Health Service and Social Work, University of Trnava; Institute of Pharmacology, The Faculty of Medicine, Komenského University, Bratislava; Environment, a.s. Nitra. METHODS: During the period of 6 years our patients were divided into subgroups according to the type of hypertension in pregnancy and the severity of the illness which was the basis for used antihypertensive drugs. One of the topics of the study was to provide monitoring of adverse events by mother and possible drug influence on their new-born has been evaluated by investigating the week of birth, weight and length of the new-born and evaluating of Apgar score. RESULTS: In the subgroup of 48 women treated by acebutolol we have confirmed the efficacy and safety of this antihypertensive drug without any clinically significant effect on the quality of life of their new-born and evaluating of Apgar score. CONCLUSION: With regard to the results of our clinical study we can consider acebutolol to be effective and safe antihypertensive drug in the treatment of hypertension in pregnancy.

Acebutolol↗
PubMed1996

Oxytocin affects the release of steroids, insulin-like growth factor-I, prostaglandin F2alpha and cyclic nucleotides by human granulosa cells in vitro.

The aim of the present experiments was to examine the effects of oxytocin (1-10 000 ng/ml) on hormone and cyclic nucleotide secretion by human granulosa cells cultured in a serum-supplemented medium. The release of progesterone, oestradiol, insulin-like growth factor-I, prostaglandin F2alpha, cAMP and cGMP into the incubation medium was analysed by radioimmunoassay. An inhibition of progesterone, but not of oestradiol release was observed. Oxytocin also stimulated insulin-like growth factor-I, prostaglandin F2alpha, cAMP and cGMP output. The results suggest an involvement of oxytocin in the autocrine/paracrine regulation of steroid, insulin-like growth factor, prostaglandin and cyclic nucleotide release by human ovarian cells.

Adult↗
PubMed1996

[Treatment of pelvic endometriosis with goserelin, an LHRH agonist].

OBJECTIVE: To determine the safety and effectiveness of ZOLADEX depot (goserelin) in the therapy of endometriosis (study No. 9393CZ/0001). MATERIAL AND METHODS: A multicentric prospective open study of 60 women with endometriosis. R-AFS score of pelvic endomemtriosis was established in patients suffering from pelvalgia, dyspareunia and dysmenorrhoea during pre-treatment laparoscopy. The effect of Zoladex depot applied subcutaneously were evaluated by gynaecological and laboratory examinations, including assessment of subjective complaints every four weeks in the course of six months. Laparoscopic check-up was carried out immediately after terminating the therapy. RESULTS: (1) The average total score of subjective complaints decreased by 90% after 24 weeks of treatment. (2) Treatment was successful in 67% patients, i.e. laparoscopic examination showed a decrease of the R-AFS score by at least 50%. In 28% treated women visible endometrial foci disappeared completely. (3) The most frequent side-effects of therapy are associated with hypoestrogenic effects of GnRH agonists. Vasomotor instability, flushes and night hyperidrosis occurred in 93% and 90% treated women, respectively. CONCLUSION: Goserelin acetate is a synthetic GnRH agonist suitable for safe and effective therapy of pelvic endometriosis. The administration of 3.6 mg goserelin every day for 24 weeks reduced markedly the size of endomerial foci and painful symptoms.

Adult↗
PubMed1994

Laparoscopy without use of the Veress needle: results in a series of 1,600 procedures.

OBJECTIVE: To describe a method of laparoscopy without use of the Veress needle and to analyze the associated complications. DESIGN: We retrospectively reviewed all 1,600 laparoscopic procedures performed in the Department of Obstetrics and Gynecology at Nitra Hospital from Aug. 23, 1989, to Jan. 31, 1994. MATERIAL AND METHODS: Our laparoscopic techniques were outlined, and our complication rate was assessed and compared with that from the literature. RESULTS: In the 1,600 procedures, 6 complications occurred (0.375%)--4 technical failures in extremely obese patients, 1 case of umbilical granuloma 2 months after the procedure, and 1 case of omental prolapse on the sixth postlaparoscopic day. CONCLUSION: Results from this series of procedures suggest that laparoscopy without use of the Veress needle is simple, safe, efficient, and easy to learn.

Female↗
PubMed1992

Morphological changes of Neisseria gonorrhoeae in amniotic fluid of pregnant women in their midtrimester.

Ten selected samples of amniotic fluid obtained through transabdominal amniocentesis from 10 pregnant women in their 17th-19th weeks of pregnancy were investigated for the survival of Neisseria gonorrhoeae in amniotic fluid. In 8 cases an antibacterial effect was observed with morphological changes comparable to the effect of benzylpenicillin. When N. gonorrhoeae was inoculated in amniotic fluid at 10(5)-10(7) cells/ml, it survived as an average 2 h longer than after inoculation of 10(2)-10(4) cells/ml. Electron microscopic pictures of gonococci taken after 2.6 and 24 h incubation in amniotic fluid correlated with the growth curves. Electron microscopically there were marked morphologic changes of N. gonorrhoeae, viz. vacuolar degeneration of their cytoplasm with a damage to the bacterial wall up to its complete destruction and lysis of the cell.

Amniotic Fluid↗
PubMed1988

[The effect of amniotic fluid on Neisseria gonorrhoeae].

This study shows the antibacterial effect of amniotic fluid collected on 10 women from 32 to 38 weeks. Two techniques were used. The first is a quantitative analysis of a bacterial culture with amniotic fluid as compared to controls, with inhibition by the amniotic fluid demonstrated. The second is a morphologic analysis of gonorrheal cultures by electron microscopy, showing alteration of the bacteria when amniotic fluid is present. These facts are of interest when one tries to understand the discrepancy between high rates of discovery of bacteria during pregnancy and low rates of intra-amniotic infection by Neisseria gonorrhoeae.

Amniotic Fluid↗