National Anaesthesia Day activities.
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Anesthesiology↗
Biomedical subjects
M Mok
Publications and source records attributed to M Mok.
A profile of Little Athletes' injuries and the prevention methods used.
This study provides a profile of injuries sustained by Little Athletes and the injury prevention strategies they employed. The sample comprised 388 registered Little Athletes from seven Sydney New South Wales Clubs. Results from the survey and fieldwork suggested that at least one out of five Little Athletes had one or more injuries resulting from their participation in the current season. However, athletes aged under 11 to under 15 years were more likely to be injured than those in the younger age groups. The older athletes were also likely to have sustained more than four injuries during the season. Athletes reported low use of both warm up and warm down methods. Coaches, age managers and parents were portrayed as the key providers of information concerning injury prevention strategies. It was therefore noted that a valuable opportunity exists to create and design an innovative, age progressive educational package for Little Athletes, parents and coaches.
Adolescent↗
Improvement in diabetes control with a monitoring system based on a hand-held, touch-screen electronic diary.
We conducted a six-month prospective interventional crossover study examining a computerized diabetes monitoring system (DMS) that conveyed dietary information. The objectives were to compare glycaemic control between intervention and control periods, and to assess patients' acceptance of the DMS. Nineteen patients were randomized into two groups, each using the DMS for three months and serving as the control group for another three months. The patients recorded information about their meal portions and blood glucose readings in a hand-held electronic diary. After transmitting the data to the DMS through a telephone modem, the patients received immediate feedback about the carbohydrate, protein and fat content of the meal, as well as the calorie content. A significant improvement in glycaemic control was achieved during intervention compared with control periods (mean HbA1C reduction of 0.825%). The DMS was also highly acceptable: 95% patients found it easy to operate while 63% found it useful. The DMS was thus a feasible model of telemedicine in diabetes care and a larger study is warranted to examine its cost-effectiveness.
Adult↗
Postoperative coma induced by hyponatremia after laparoscopy--a case report.
Hyponatremia is a common clinical entity which may occur during the course of many medical illnesses. However disastrous sequelae or even death may develop in young, generally healthy patients who receive simple elective surgery. Here we present a case of a 34-year-old female, without past history of cardiopulmonary or renal disease, after undergoing laparoscopic surgery developed mental status changes and lapsed into coma on the second postoperative day. She was found to have a serum sodium level of 110 mEq/L. After careful treatment and a protracted hospital stay, the patient recovered uneventfully. The pathophysiology of postoperative hyponatremia is discussed and attention is called to the special vulnerability of menstruant women who carry a much increased risk of mortality and morbidity associated with hyponatremia.
Adult↗
Rasch models overview.
This overview of Rasch measurement models begins with a conceptualization of our continuous experiences that are often captured as discrete observations. It goes on to discuss the properties that are require of measures if they are to transcend the occasion in which they were collected, and concludes with a discussion the spiral of inferential development. This is followed by a discussion of the mathematical properties of the Rasch family of models that allow the transformation of discrete deterministic counts into continuous probabilistic abstractions on which science is based. The overview concludes with a discussion of six of the family of Rasch models, Binomial Trials, Poisson Counts, Rating Scale, Partial Credit, and Ranks and the types of data for which these models are appropriate.
Binomial Distribution↗
Analysis of breast milk to assess exposure to chlorinated contaminants in Kazakstan: PCBs and organochlorine pesticides in southern Kazakstan.
Organochlorine pesticides (OC) and polychlorinated biphenyls (PCBs) were measured in samples of breast milk taken from 92 donors representative of regional populations in southern Kazakstan. The World Health Organization protocol for assessing levels of chlorinated contaminants in breast milk was followed. The most prevalent OC residues were beta-hexachlorocyclohexane (beta-HCH), p,p'-DDE, p,p'-DDT, hexachlorobenzene, and alpha-HCH. The measured levels of beta-HCH were among the highest reported in the published literature. Data from Aralsk, near the Aral Sea, indicated continuing DDT exposure. Overall PCB-toxic equivalent levels (22 pg/g fat) were similar to those reported in industrialized European countries. PCBs were highest in Atyrau in the Caspian oilfields.
Adolescent↗
The social and gender composition of nursing students in Australia: a comparison between 1987-1990 and 1995 recruits.
This national study compares the social and gender characteristics of an earlier group of 1551 students in higher-education programs in 1987 and 1990 with the national study group of 2295 students sampled in 1995. Using a specially constructed socio-economic variable for comparison, the analyses demonstrated a significant difference in the socio-economic level of the household for the female but not the male group. There was a significant difference in the proportion of males entering nursing between the earlier and latter groups. Further, in the latter group, these males were more likely to enter nursing programs directly from school. Members of the female group in the latter sample were more likely to have attended a school in a less populated area, come from households with a reduced family size, have mothers who were earning an income and have mothers who had achieved a higher level of education than was found in the earlier group. Logic analysis revealed that there was a significant interaction between the household variables, socio-economic status, number of siblings and income received by the mother of the respondents in the early and latter groups for females but not for males. This interaction for the female group, plus the finding that members of the latter group were more likely than other university students to defer their Higher Education Contribution Scheme (HECS) payments, suggests that if politicians were to make changes to the HECS it may affect the delicate social balance currently achieved in nursing recruitment in Australia.
Australia↗
The pursuit of excellence in nursing care: what does it mean?
This study explores the meaning of excellence in nursing care held by nurses. Qualitative data were collected from 156 undergraduate and postgraduate nurses by means of an open-ended questionnaire. Respondents were asked to reflect on practice which enabled a clear understanding of the meanings given to excellence in nursing care. In addition, details of their nursing background, level of nursing experience, and demographics were sought. Responses were content-analysed for themes by three coders. Four major themes emerged: (1) professionalism, (2) holistic care, (3) practice, (4) humanism. The fourth theme comprised three sub-themes: (a) enabling personal qualities, (b) nurse patient relationships, and (c) nurse-health team relationships. Responses revealed that professionalism constituted an all-encompassing theme subsuming all others. Findings suggested that the client is the central focus of excellent nursing care at all times. According to these respondents, nurses who deliver excellent nursing care implement nursing in a professional and competent manner, demonstrate a holistic approach to caring, possess certain personal qualities which enhance practice, and relate to patients, families, peers, hospital administrators and community members in a competent, cooperative manner. An important aspect of this study is the differential quality of responses provided by respondents, who were students enrolled in a nursing degree programme compared to respondents who were state-registered nurses and concurrently undertaking higher degree studies. The final result is suggestive of the stages of transition through which nurses pass in their quest for excellence.
Adolescent↗
The role of speech processes and memory in reading disability.
The performance of poor and average readers on cognitive tasks comprising naming time, speech rate, and word series recall and on a phonemic segmentation task was examined in the first part of this study by computing relationships among the tasks and their relation to reading. In the second part of this study, we investigated the performance of the poor and average readers in detail on naming time by analyzing the audiotaped protocols on a computer equipped with a sound system and an analysis program. The subjects were third- and fourth-grade students. Results showed that naming time and speech rate were dependable measures of phonological coding skills and were better predictors of word decoding than were phonemic segmentation and word recall. Poor readers were slower in each of the six rows of the naming-time task, and they paused longer between the rows. A need for studying the development of the cognitive skills along with word decoding is discussed.
Child↗
Spacing and orientation of bipartite DNA-binding motifs as potential functional determinants for POU domain factors.
Investigation of the large POU domain family of developmental regulators has revealed a molecular mechanism by which highly related transcription factors sharing common DNA-binding motifs act to functionally discriminate their cognate DNA sequences. Studies of two classes of neuron-specific POU domain factors (III and IV) indicate that functional specificity on their native response elements is achieved by accommodating different nucleotide spacing between variably oriented bipartite core DNA-binding motifs. The preferred orientation of the POU-specific domain of the neuronal factors on their native response elements appears to be opposite that of Pit-1 and Oct-1. Members of POU-III (Brn-2) class exhibit remarkable flexibility in DNA site recognition (tolerating core motifs spaced by 0, 2, or 3 nucleotides), whereas POU-IV (Brn-3) class is highly constrained (tolerating core motifs with a spacing of 3 nucleotides). The molecular determinant of the constraint in DNA site selection appears to be imparted by 3 amino acid residues in the amino-terminal basic region in concert, with helix 2 of the POU homeo domain which together are involved in minor groove and possibly phosphate backbone contacts. Similar mechanisms may underlie differential flexibility in spacing and orientation for diverse families of transcription factors.
Amino Acid Sequence↗
Identification of Dirofilaria immitis larval antigens with immunoprophylactic potential using sera from immune dogs.
Previous research has demonstrated that dogs that received chemically abbreviated Dirofilaria immitis larval infections were significantly immune to challenge infections. Sera from those immune animals have been effective in passively transferring larval killing and stunting. In the present study, sera from immune and control animals were used to screen various Ag subsets for unique Ag. Through Western blot analysis of larval extracts and excretory-secretory products, and immunoprecipitation of metabolically labeled proteins and larval surface Ag, it was determined that as many as 12 molecules were uniquely recognized by protective immune sera. A 39-kDa molecule was present in both soluble lysates of third- and fourth-stage larvae and larval excretory-secretory products; it was recognized by each of the immune dogs and by none of the infected or uninfected control animals. The 39-kDa molecule appeared to be absent from adults and microfilariae of the parasite. In addition to the unique recognition by immune dog sera, larval stage specificity of this molecule suggests that it may be useful as a vaccine candidate.
Animals↗
Solubilization of epicuticular antigen from Dirofilaria immitis third-stage larvae.
The solubilization of epicuticle from third-stage (L3) Dirofilaria immitis larval cuticles was investigated. Cuticles collected after L3 had molted were incubated in 1.5% sodium dodecyl sulfate (SDS) at 37 degrees C with vigorous shaking. Solubilization of epicuticular layers was accomplished as demonstrated by electron microscopy. Diminished binding of an epicuticular specific monoclonal antibody (DIM-229) was seen when SDS-treated cuticles were compared to untreated cuticles in an indirect fluorescence antibody assay. Cuticles which were extracted further by boiling in 1.5% dithiothreitol (DTT) produced less protein than cuticles solubilized in SDS. Both extracts reacted with DIM-229 in an indirect enzyme-linked immunosorbent assay, indicating retention of antigenic reactivity of the solubilized epitope. SDS-polyacrylamide gel electrophoresis of SDS-derived antigens revealed, after silver staining, proteins from 12 to 77 kDa and only 1 band at 15 kDa for SDS-treated cuticles boiled in DTT. Western blot analyses of the extracts with DIM-229 were inconclusive.
Animals↗
[Combined effect of intrathecal morphine and nalbuphine for postoperative analgesia].
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Adult↗
The Escherichia coli preprimosome and DNA B helicase can form replication forks that move at the same rate.
A DNA replication system was developed that could generate rolling-circle DNA molecules in vitro in amounts that permitted kinetic analyses of the movement of the replication forks. Two artificial primer-template DNA substrates were used to study DNA synthesis catalyzed by the DNA polymerase III holoenzyme in the presence of either the preprimosomal proteins (the primosomal proteins minus the DNA G primase) and the Escherichia coli single-stranded DNA binding protein or the DNA B helicase alone. Helicase activities have recently been demonstrated to be associated with the primosome, a mobile multiprotein priming apparatus that requires seven E. coli proteins (replication factor Y (protein n'), proteins n and n'', and the products of the dnaB, dnaC, dnaG, and dnaT genes) for assembly, and with the DNA B protein. Consistent with a rolling-circle mechanism in which a helicase activity permitted extensive (-) strand DNA synthesis on a (+) single-stranded, circular DNA template, the major DNA products formed were multigenome-length, single-stranded, linear molecules. The replication forks assembled with either the preprimosome or the DNA B helicase moved at the same rate (approximately 730 nucleotides/s) at 30 degrees C and possessed apparent processivities in the range of 50,000-150,000 nucleotides. The single-stranded DNA binding protein was not required to maintain this high rate of movement in the case of leading strand DNA synthesis catalyzed by the DNA polymerase III holoenzyme and the DNA B helicase.
Bacterial Proteins↗
Formation of rolling-circle molecules during phi X174 complementary strand DNA replication.
The primosome is a mobile multiprotein priming apparatus that requires seven Escherichia coli proteins for assembly (the products of the dnaB, dnaC and dnaG genes; replication factor Y (protein n'); and proteins i, n, and n"). While the primosome is analagous to the phage T7 gene 4 protein and phage T4 gene 41/61 proteins in its DNA G-catalyzed priming function, its ability to act similarly also as a DNA helicase has remained equivocal. The role of the primosome in unwinding duplex DNA strands was investigated in the coliphage phi X174 SS(c)----replicative form DNA replication reaction in vitro, which requires the E. coli single-stranded DNA binding protein, the primosomal proteins, and the DNA polymerase III holoenzyme. Multigenome-length, linear, double-stranded DNA molecules were generated in this reaction, presumably via a rolling circle-type mechanism. Synthesis of these products required the presence of a helicase-catalyzed strand-displacement activity to permit multiple cycles of continuous complementary (-) strand synthesis. The participation of the primosome in this helicase activity was supported by demonstrating that other SS(c) DNA templates (G4 and alpha-3), which lack primosome assembly sites, failed to support significant linear multimer production and that replication of phi X174 with the general priming system (the DNA B and DNA G proteins and DNA polymerase III holoenzyme) resulted in a 13-fold lower rate of linear multimer synthesis.
Bacteriophage phi X 174↗
In vitro culture of Dirofilaria immitis third- and fourth-stage larvae under defined conditions.
The objective of the present study was to define culture conditions under which larval Dirofilaria immitis would molt, grow, and survive. Third-stage larvae (L3) survived for over 3 wk with a molt rate of up to 95% in a variety of media supplemented with fetal calf serum. Bovine albumin, added to several media at concentrations of 10-30 mg/ml, also proved to be an effective culture supplement for the induction of molting and for supporting larval survival. Two gas phases were tested, 5% CO2/95% N2 and 5% CO2/air; no differences were noted in larval development based on gas phase. Larvae, maintained in media with FCS or albumin for 48 hr, were capable of completing the molting process and growing in length in unsupplemented media. If the temperature at which cultures were maintained was changed from 37 C to 27 C, L3 did not molt but did survive for several weeks. Two factors required for larval D. immitis molting and growth have been identified, temperature of approximately 37 C and the presence of albumin in the culture medium. The defined culture system developed for D. immitis L3 may provide a source for collection of excretory-secretory antigens, which could prove useful in immunodiagnosis or immunoprophylaxis as well as provide a means of studying the process and requirements of filarial larval molting.
Animals↗
Thermotaxis in third and fourth-stage Dirofilaria immitis larvae.
Third-stage and fourth-stage Dirofilaria immitis larvae exhibited positive thermotaxis when placed in a thermal gradient. Negative thermotaxis was not observed. Positive thermotaxis may be important for the successful transmission and for directing third and fourth-stage larval migration toward predilection sites in the host.
Aedes↗
Premature termination by human RNA polymerase II occurs temporally in the adenovirus major late transcriptional unit.
We have recently demonstrated pausing and premature termination of transcription by eucaryotic RNA polymerase II at specific sites in the major late transcriptional unit of adenovirus type 2 in vivo and in vitro. In further developing this as a system for studying eucaryotic termination control, we found that prematurely terminated transcripts of 175 and 120 nucleotides also occur in adenovirus type 5-infected cells. In both cases, premature termination occurs temporally, being found only during late times of infection, not at early times before DNA replication or immediately after the onset of DNA replication when late gene expression has begun (intermediate times). To examine the phenomenon of premature termination further, a temperature-sensitive mutant virus, adenovirus type 5 ts107, was used to uncouple DNA replication and transcription. DNA replication is defective in this mutant at restrictive temperatures. We found that premature termination is inducible at intermediate times by shifting from a permissive temperature to a restrictive temperature, allowing continuous transcription in the absence of continuous DNA replication. No premature termination occurs when the temperature is shifted up at early times before DNA replication. Our data suggest that premature termination of transcription is dependent on both prior synthesis of new templates and cumulative late gene transcription but does not require continuous DNA replication.
Adenoviridae↗