Biomedical subjects

M Molnár

Publications and source records attributed to M Molnár.

116 records · Page 7Linked to original sources

PubMedDate not supplied

Cytokine-initiated signal transduction in human myometrial cells.

PROBLEM: The objectives of this study were to evaluate interleukin-1 (IL-1) binding and some postreceptor actions of this cytokine and tumor necrosis factor (TNF) in human myometrial cells (HMC). METHOD: Monolayer cultures of HMC were used to characterize binding and to measure cyclic (c)AMP, prostaglandin (PG)E2, and PGI2 production. Membrane preparations were used to assess ADP-ribosylation and for immunoblotting. RESULTS: HMC were found to specifically bind [125I]IL-1 with an apparent Kd of 2 x 10(-10) M. Incubation of HMC with IL-1 or TNF caused a time-dependent and dose-dependent accumulation of cAMP, as well as a significant potentiation of forskolin-promoted cAMP production. These cytokines also increase PGE2 and PGI2 output, independently of the activation of adenylyl cyclase. IL-1 treatment had no measurable effect either on cholera toxin-mediated and pertussis toxin-mediated ADP-ribosylation, or on the amount of Gi proteins, as assessed by immunoblotting using a polyclonal antibody. CONCLUSIONS: It is suggested that IL-1 and TNF may activate one or more isoforms of the catalytic component of adenylyl cyclase, raising intracellular cAMP.

Adenosine Diphosphate Ribose↗

PubMedDate not supplied

Towards the possible clinical application of the mismatch negativity component of event-related potentials.

The mismatch negativity (MMN) is an event-related potential component that signifies neurophysiological processing of fine acoustic differences. The MMN indicates attention-independent change detection, reflects auditory sensory memory and provides a physiological measure of difference sensitivity. This paper will provide an overview of current research where results gained by MMN testing in different patient groups were central to the interpretation of an assumed abnormality of processing or storing acoustic features.

Adult↗

PubMedDate not supplied

Effects of administration of different intravenous lipid emulsions on plasma LP-X concentrations in the rat.

BACKGROUND: Prolonged parenteral nutrition with lipid emulsions is essential to provide sufficient energy supply and to avoid essential fatty acid deficiency in preterm infants. However, chronic administration of lipid emulsions may lead to the development of pathological plasma lipid and LP-X concentrations. The aim of this study was to evaluate the relative importance of the phospholipid-triglyceride (PL-TG) ratio and the source of phospholipid in lipid emulsions, with respect to plasma lipid and LP-X levels. METHODS: Rats were infused for 9 days with IV lipid emulsion containing 10% (IL-10) or 20% (IL-20) egg lecithin or Lipofundin containing 20% soya lecithin (LF), with PL-TG ratios of .12, .06, and 0.75, respectively. RESULTS: LF significantly increased plasma triglyceride concentration (p < .01), whereas the rise in cholesterol levels observed with all emulsions was primarily caused by the increase in low-density lipoprotein cholesterol concentrations. The plasma phospholipid concentration was increased most by IL-10 (p < .005). There was a strong correlation between the PL-TG ratio of emulsions and the developing plasma phospholipid and LP-X concentrations (r2 = .91 and .96, respectively), despite the different origin of phospholipids in the emulsions, suggesting that it is the PL-TG ratio, rather than the source of phospholipids in lipid emulsions that primarily influences developing plasma lipid and LP-X concentrations. CONCLUSION: These results indicate that the administration of lipid emulsions with lower PL-TG ratios should be considered, to avoid the development of pathological plasma lipoprotein concentrations.

Analysis of Variance↗

PubMedDate not supplied

Prevalence, course and risk factors of diabetic nephropathy in type-2 diabetes mellitus.

UNLABELLED: Contrary to the well-known features of diabetic nephropathy (DNP) in type-1 diabetic patients (pts), the prevalence, course and risk factors of DNP in in type-2 diabetic pts are not clear. The aim of the present study was to assess the prevalence of microalbuminuria (MA) and macroalbuminuria (MAA), their relationship with other diabetic complications and with some known cardiovascular risk factors in 200 in type-2 diabetic pts (100 females and 100 males). 68 pts (33%) were normalbuminuric (NA), 55 (27.5%) had MA and 77 (38.5%) had MAA. There was no significant difference among these three groups in age, BMI or the time actually elapsed since the diabetes and hypertension were diagnosed. BMI was high in each group (28.8 +/- 5.29, 28.0 +/- 5.2 and 29.8 +/- 4.6 kg/m2 mean +/- SD). 65% of pts with NA, 77% of those with MA and 81% of pts with MAA had hypertension. MAA pts were more frequently smokers and former smokers, than MA and NA pts (56% vs 32% and 22%). Average GRF values (ml/min/1.73 m2) were 71.9 +/- 26.8 in NA pts, 82.3 +/- 36.8 in MA pts and 56.3 +/- 32 in MAA pts. There was no significant correlation between the urinary albumin excretion (UAE) and glycemic control, serum (se) cholesterol and se HDL cholesterol. At the same time UAE showed a significant positive correlation with se trigliceride (P < 0.01), se uric acid (P < 0.01) and se creatinine (P < 0.01) while a significant negative correlation was found with GFR (P < 0.01). Diabetic non-proliferative retinopathy (RP) was detected even in NA pts (27%) while 51% of MAA pts were without RP. 56% of NA pts, 57% of MA pts and 93% of MAA pts had macroangiopathy. CONCLUSIONS: (1) renal function can be impaired even in type-2 diabetic pts with NA and MA, (2) well-known cardiovascular risk factors seem to have a close relation with renal damage in type-2 diabetes (3) renal lesions in type-2 diabetic pts may be caused by diseases other than diabetes (e.g. arteriosclerosis, hypertension) (4) unlike in type-1 diabetes, where the strict glycemic control is the main preventive factor of DNP, in type-2 diabetes, the control of hypertension, hyperlipidemia, obesity, hyperuricemia may have priority.

Adult↗

PubMedDate not supplied

Correlation dimension changes of the EEG during the wakefulness-sleep cycle.

The mathematical tools of chaos theory make the quantification of a time series such as the electroencephalogram possible by calculating its correlation dimensions. In this study, the electroencephalogram was recorded from the vertex in cats during the wakefulness-sleep cycle and its correlation dimension was determined. For the estimation of the correlation dimension a new method, the point-correlation dimension was used. This method is more accurate than others currently used for tracking non-stationarities within the data. The point-correlation dimension was higher during the alert state than in slow wave sleep. It is suggested that during the alert state a different type of pacemaker activity takes over in the generation of the electroencephalogram.

Animals↗

PubMedDate not supplied

[General principles of planning fixed prostheses on implants].

The planning of fixed prostheses comprising also implant abutment is determined by that, as regards loadability, none of the implant kinds can be considered as first rate abutment tooth. At planning bridges the procedure according to the prosthetic principles related to weaker or damaged periodontium teeth is employed.

Dental Abutments↗

PubMedDate not supplied

[Stereotaxic destruction of the ventrolateral thalamic nucleus in combination with the transplantation of fetal and xenogeneic tissue in Parkinson's disease].

Four patients with Stage III-IV Parkinson's disease were operated on. Stereotactic cryodestruction of the ventrolateral nucleus was made, followed 10 minutes later by implantation of mixed fetal and xenogenic nerve tissue cells. The nerve cells-the fetal part-are obtained from human fetuses in the age range of 14-18 weeks of development. Tissues from the ventral portion of the midbrain and basal ganglia of the forebrain were used. Neurogenic layings of Notch Drosophila melanogaster mutants serve as a xenograft. The mixture of nerve cells were prepared in ratios of 1000:20 and 1000:12. The follow-up of the patients lasted a year. Relapses were absent. The effect occurred on the operating table (in the combination of nuclear destruction and nerve cell transplantation) and only 6 months later only during transplantation of nerve cells (without destruction). An attempt of transplanting the above fetal xenogenic mixture of nerve cells to patients previously undergone 2 ineffective stereotactic operations provided no significant clinical effect. The concurrent destruction of ventrolateral nuclei in combination with fetal xenogenic tissue grafting is considered to be promising.

Animals↗

PubMedDate not supplied

Effect of phenothiazines, benzo[a] phenothiazines, benz[c]acridines and Pentaglobin on endotoxin.

The endotoxin neutralizing effects of several phenothiazines, benzophenothiazines and Pentaglobin (control) were investigated by spectrophotometry, tumor necrosis factor (TNF) induction and the conventional Limulus test. In animal experiments, some beneficial effects of complex forming compounds were found, however, the compounds could not completely inactivate the biological effect of endotoxin in the Limulus test. The complex formation between endotoxin and the compounds were revealed in spectrophotometry. The TNF inducing effect of compound-endotoxin complexes was markedly reduced by some phenothiazines and benzo[a]phenothiazines in leukocytes. Benz[c]acridines and Pentaglobin could not neutralize completely the TNF induction of E. coli endotoxin. The recent findings indicate that multifocal effects of phenothiazines and benzophenothiazines can be responsible for anti-endotoxin action in vivo. Hypotensive action in experimental animals was reduced by some phenothiazines in some preliminary experiments.

Acridines↗