Serological survey of markers of infection with viral hepatitis among the Yukpa Amerindians from western Venezuela.
Explore the source record for details and available documents.
Hepatitis B↗
Biomedical subjects
M Monzon
Publications and source records attributed to M Monzon.
Infection of macaque monkeys with simian immunodeficiency virus from African green monkeys: virulence and activation of latent infection.
The virulence of three isolates of simian immunodeficiency virus from African green monkeys (SIVagm) was studied in rhesus and pigtailed macaques. None of 15 rhesus monkeys and one of four pigtailed monkeys died from infection during the time they were studied (up to 33 months). SIVagm was only isolated from rhesus monkeys for up to 2 months after inoculation. However, when these animals were secondarily infected with Simian acquired immunodeficiency syndrome retrovirus type 1 (SRV-1), SIVagm was activated and isolated. Dual infection caused increased mortality.
Animals↗
Development of the ventral striatum in the lizard Gallotia galloti.
The ventral striatum nucleus (VS) begins development at Stage 31 (E. 31) from the neuroblasts which proceed from the cellular proliferation of both the ventral and terminal sulci. The ultrastructural features of the neuroblasts of VS between E. 31 and E. 34 have the aspect of immature cells, but as from E. 38 neuronal maturity is gradual until hatching. At E. 34 cellular death occurs. The first degenerated cells belong to Type I (nuclear degeneration) of the pycnotic cells; as from E. 40 cytoplasmic degeneration appears. Vascularisation starts at E. 35 and from E. 38 the first synaptic contacts are observed, especially those of the axodendritic type.
Animals↗
Typing of herpes simplex virus by capture biotin-streptavidin enzyme-linked immunosorbent assay and comparison with restriction endonuclease analysis and immunofluorescence method using monoclonal antibodies.
A sensitive enzyme-linked immunosorbent capture assay using biotin and streptavidin (capture B/SA ELISA) was developed using type-specific monoclonal antibodies for typing of herpes simplex virus. Rabbit anti-herpes simplex virus immunoglobulin G was used as the capturing antibody, and biotin-linked type-1-specific mouse monoclonal antibody or rabbit type-1- or type-2-specific polyclonal antibody served as the detecting antibody. The captured antigen was detected by an ELISA with alkaline phosphatase-conjugated streptavidin, which reacted with biotin molecules on the detector antibody. The capture B/SA ELISA was compared with other methods for efficiency and reliability in typing. Results obtained by restriction endonuclease digestion of the radiolabeled viral genome were used to determine the type (1 or 2) of clinical isolates. These results were then used as a reference for determining the accuracy of the capture B/SA ELISA, as well as that of the immunofluorescence method, both of which are easily adaptable for use in the clinical laboratory. The three methods were in perfect agreement. It was determined that both the capture B/SA ELISA and the immunofluorescence method using monoclonal antibodies provided typing results with 100% specificity and 100% sensitivity and thus were accurate and reliable. However, the ELISA was the method of choice because of its simplicity, rapidity, and use of nonradioisotopic reagents.
Antibodies, Monoclonal↗
Cell death in the normal development of Gallotia galloti mesencephalon (Reptilia Lacertidae). An ultrastructural study.
We have studied the development of the mesencephalon of Gallotia galloti and we have observed two classes of cellular death: 'nuclear' or 'Type I' and 'cytoplasmatic' or 'Type II'. The first appears in stages around E-34 and the second one is observed from stages around hatching to the adult lizard. The degenerative cells have been observed in the profundus, torus semicircularis, the 5th pair of the trigeminal nerve nuclei as well as the 4th and 5th layers of the optic tectum where this last nucleus mentioned is situated.
Aging↗
Neuronal cilia in the embryonic thalamus and striatum of Gallotia galloti (Reptilia, Lacertidae).
Cilia are a common characteristic of the embryonic neurons of the thalamus and striated centers of Gallotia galloti from E.35. The ciliogenesis process occurs around the stages E.33 and E.34. The ciliary morphology shows no substantial variations between the different stages and the ciliary structure in the embryonic period is 8 + 1. On the other hand, the adult cilia has a 9 + 2 arrangement of microtubules. We do not rule out the possibility of other axonemes.
Animals↗