Biomedical subjects

M Moors

Publications and source records attributed to M Moors.

15 recordsLinked to original sources

PubMed1997

Carbon-source regulation of virulence gene expression in Listeria monocytogenes.

All known virulence genes of Listeria monocytogenes are under positive regulation by the transcription factor PrfA. Previous work employing the L. monocytogenes strain NCTC7973 suggested that the disaccharide cellobiose might serve as a specific "signature molecule' which functions to prevent activation of the PrfA-controlled regulon in a soil environment. We have examined three other L. monocytogenes strains, 10403S, LO28 and EGD, all commonly regarded as wild-type isolates, and find that NCTC7973 is anomalous with respect to the effect of carbohydrates on the expression of PrfA-controlled gene expression. In the case of 10403S, LO28 and EGD, several other readily metabolized mono- and disaccharides are as effective as cellobiose in repressing expression of the PrfA-controlled gene hly, indicating that the cellobiose effect is not specific, and suggesting that NCTC7973 may be a partially deregulated variant. Moreover, concentrations of cellobiose and other sugars required for repression of hly expression (> 1 mM) were found to significantly enhance growth of L. monocytogenes cultures, suggesting that the repression phenomenon probably results from a metabolic effect of sugar utilization rather than a signal-sensing response. Thus the previously reported cellobiose effect may reflect an aspect of a more global mechanism of catabolite repression in L. monocytogenes. Although cellobiose represses expression of hly and plcA at the level of transcript accumulation, quantitative Western blot analysis indicates that cellobiose has no effect on PrfA levels. These results are consistent with a model in which PrfA activity is controlled by interaction with a hypothetical cofactor, the synthesis or depletion of which is responsive to the presence of readily metabolized carbohydrates.

Bacterial Proteins↗

PubMed1996

Elimination of the listeriolysin O-directed immune response by conservative alteration of the immunodominant listeriolysin O amino acid 91 to 99 epitope.

A major H2-Kd-presented epitope for antilisterial cytotoxic T lymphocytes (CTLs) is the nanomer peptide which corresponds to the amino acid 91 to 99 (aa91-99) sequence from listeriolysin O (LLO). Although the LLO sequence contains at least five additional nanomer peptides which also satisfy the H2-Kd binding motif, aa91-99 is the only LLO-derived target peptide that is recognized by antilisterial CTLs following infection of BALB/c mice with Listeria monocytogenes. In order to investigate further the immunodominance of the LLO aa91-99 epitope following endogenous processing of LLO, we introduced a point mutation in hly (the gene for LLO) which results in a conservative Y-to-F substitution for the anchor residue at position 2 within the aa91-99 sequence. This "92F" L. monocytogenes mutant produces biologically active LLO and is phenotypically indistinct from wild-type L. monocytogenes in terms of intracellular growth in vitro and virulence in vivo. BALB/c mice actively immunized with the 92F L. monocytogenes mutant are protected against challenge with wild-type L. monocytogenes. Antilisterial CTLs from mice immunized with the 92F mutant lyse targets infected with L. monocytogenes; however, these CTLs do not lyse target cells pulsed with either the LLO aa91-99 peptide, other LLO-derived peptides which satisfy the H2-Kd binding motif, or a peptide corresponding to the LLO aa91-92F-99 sequence. Target cells pulsed with the LLO aa91-92F-99 peptide are, however, lysed by wild-type LLO aa91-99-specific cytotoxic cells. Thus, a conservative amino acid change in the first anchor residue of the immunodominant aa91-99 sequence of LLO eliminates the induction of the cytotoxic cell response to this epitope as well as to any of the other candidate LLO-derived peptides which fit the H2-Kd binding motif. The lack of anti-LLO-specific CTLs following immunization with the 92F mutant does not appear, however, to influence the protective antilisterial immune response.

Amino Acid Sequence↗

PubMed1994

Knowledge-based system for the automated solid-phase extraction of basic drugs from plasma coupled with their liquid chromatographic determination. Application to the biodetermination of beta-receptor blocking agents.

Techniques for the preparation of biological samples are often based nowadays on solid-phase extraction (SPE). The different SPE steps can be performed automatically on disposable extraction cartridges (DECs) by means of a sample processor. A knowledge-based system was developed to facilitate the development of fully automated methods for the solid-phase extraction of relatively hydrophobic basic drugs from plasma, coupled with their determination by high-performance liquid chromatography (HPLC). The DEC filled with 50 mg of cyanopropyl-bonded silica phase is first conditioned with methanol and buffer solution (pH 7.4). After sample application, the DEC sorbent is washed with the same buffer. The analytes are then desorbed with an appropriate eluent and the eluate is finally diluted with the same buffer as used in the HPLC mobile phase before injection. Under these conditions, only three variables are still to be optimized: the composition and volume of the elution solvent and the volume of buffer to be added to the eluate. On the basis of this general strategy, a decision tree providing information about suggested starting conditions and guidelines for the optimization of the three variables was developed and implemented by use of a hypermedia software. This didactic expert system was evaluated using several beta-receptor blocking agents as model compounds and the operating conditions obtained for the automated SPE of these compounds are presented. A method for the determination of propranolol in plasma using the SPE conditions deduced from the knowledge-based system was validated. The absolute recovery of propranolol is ca. 93% and the limit of detection is 1.3 ng ml-1. The mean within-day and between-day reproducibilities are 2.3 and 3.6%, respectively.

Adrenergic beta-Antagonists↗

PubMed1994

Solid-phase extraction of small drugs on apolar and ion-exchanging silica bonded phases: towards the development of a general strategy.

In connection with the development of a general strategy for solid-phase extraction (SPE) of drugs, the use of the apolar octadecyl silica bonded phase and ion-exchanging phases with a benzene sulphonic acid or quaternary amine bonded functionality is investigated for the SPE of small polar drugs. This investigation was performed on a set of 15 drugs, belonging to varying pharmacological groups and with varying structures. For each analyte, its adsorption on the C18 and the ion-exchanging phase was controlled for an aqueous solution and for a spiked plasma sample. For those analytes retained on the sorbent, different elution solvents were compared. Although SPE methods could successfully be developed for some drugs, no general solution can be proposed and no solution was found for a few drugs. The main problems are that for these few drugs no sufficient retention is obtained on any SPE phase investigated or the selectivity is too low.

Amines↗

PubMed1992

Comparison in shear bond strength of orthodontic brackets between five bonding systems related to different etching times: an in vitro study.

New adhesives are continuously being marketed. The adhesive-bracket bond is still the weakest point in orthodontic bonding. A new device for the measurement of shear bond strength values (SBS) of orthodontic brackets is presented in this article. With this device, five commercial products were evaluated for their SBS: Lee Bond (LB), Concise (C), Super C (SC), Achieve-no-mix (ANM), and Panavia Ex (PE). After applying Begg brackets of laminated minimesh on lower human incisors, the specimens were thermocycled. The products investigated produced high bond strengths. The products tested showed shear bond strengths that were significantly lower than those stained with PE. The application of PE yielded the highest shear values. Nevertheless, some characteristics of this product might be clinically somewhat inconvenient. There was no significant difference in shear bond strength between 15 and 60 seconds enamel etching before bond application.

Acid Etching, Dental↗

PubMed1991

Evaluation of solid-phase extraction of basic drugs from human milk.

This article evaluates the use of commercially available cyanopropyl and octadecyl sorbents for the extraction of basic drugs from breast milk. Twenty drugs were selected from different pharmacological groups (beta-blocking agents, antidepressants, anxiolytic sedatives and neuroleptics, antihistamines, alkaloids and an anthelmintic) and subjected to a general solid-phase extraction (SPE) procedure described earlier for plasma samples. This SPE method was developed on a cyanopropyl cartridge and consisted of a conditioning step with methanol and water, the adsorption of the deproteinized matrix, washing with water and/or methanol, and finally the elution of the basic compounds with 0.1% propylamine in methanol. The extracts were further analysed by reversed-phase liquid chromatography (RP-LC). The application of SPE to human milk samples utilized cyanopropyl and octadecyl cartridges. The latter can be applied more generally because it better retains the basic compounds. For 14 out of 17 drugs extracted from breast milk, recoveries of greater than 70% were obtained. Standard deviations were, with the exception of three drugs, in the same range as those observed for plasma samples, i.e. 2-8%. The development of a strategy for SPE of drugs from human milk was difficult. For a number of drugs, in particular those present in human milk at low concentrations and/or detected in a non-selective way, matrix compounds interfered with the subsequent LC analysis. Therefore, SPE on CN or C18-sorbent for the analysis of basic compounds in breast milk was found to be useful as one of the steps in an extraction procedure, but not as a single technique. A major drawback of SPE is the batch-to-batch variation of the sorbents.

Adsorption↗

PubMed1990

Kinetics of Pseudomonas aeruginosa adhesion to 304 and 316-L stainless steel: role of cell surface hydrophobicity.

Fifteen different isolates of Pseudomonas aeruginosa were used to study the kinetics of adhesion to 304 and 316-L stainless steel. Stainless steel plates were incubated with approximately 1.5 X 10(7) CFU/ml in 0.01 M phosphate-buffered saline (pH 7.4). After the plates were rinsed with the buffer, the number of adhering bacteria was determined by a bioluminescence assay. Measurable adhesion, even to the electropolished surfaces, occurred within 30 s. Bacterial cell surface hydrophobicity, as determined by the bacterial adherence to hydrocarbons test and the contact angle measurement test, was the major parameter influencing the adhesion rate constant for the first 30 min of adhesion. A parabolic relationship between the CAM values and the logarithm of the adhesion rate constants (In k) was established. No correlation between either the salt aggregation or the improved salt aggregation values and the bacterial adhesion rate constants could be found. Since there was no significant correlation between the bacterial electrophoretic mobilities and the In k values, the bacterial cell surface charge seemed of minor importance in the process of adhesion of P. aeruginosa to 304 and 316-L stainless steel.

Bacterial Adhesion↗

PubMed1989

A model for the corrosion behavior of dental amalgams.

At high frequencies, electrochemical impedance behavior for corroding dental amalgams cannot be interpreted in terms of a simple Randles equivalent circuit. This may often be ascribed to the imperfect cell geometry resulting in a nonuniform current distribution. However, the insertion of a constant phase element (CPE) in the non-faradaic branch of the equivalent circuit offers a new scope for interpretation as the circuit provides an m-parameter corresponding to the degree of distortion. The decrease of m as a function of time was explained by a mathematical model reflecting an increasing number of pores. Moreover, that same model allows a better understanding of the passivating effect of NaHCO3 and of the differences between conventional and Cu-rich dental amalgams.

Biocompatible Materials↗

PubMed1989

A scanning electron microscopic and tensile bond strength evaluation of Gluma dentin bond application, as a function of dentinal pretreatment.

Scanning Electron Micrographs (SEM) and Tensile Bond Strength (TBS) measurements were used to evaluate the influence of three different dentinal pretreatments. A new testing device was developed for the TBS measurements. Human tooth roots were ground with a diamond bur. Tap water, Gluma Cleanser, and Neo-Sabenyl-Tubulicid were used to clean the surfaces before SEM inspection. The adhesion between root dentin and Lumifor Composite restorations was mediated by Gluma Bond. SEM observations revealed that a 30-second application of Gluma Cleanser removes the smear layer totally. Neo-Sabenyl-Tubulicid produced a very smooth surface. Application of Gluma Bond did not alter this appearance. Mean TBS values for surfaces sprayed with tap water, Gluma Cleanser, and Neo-Sabenyl-Tubulicid were 10 MPa, 7.3 MPa, and 10 MPa, respectively. There was a significant difference between group 2 (Gluma Cleanser) and the other groups. From this in vitro study, it is concluded that total removal of the smear layer with opening of the dentinal tubules (e.g., by means of Gluma Cleanser) reduces the TBS of Gluma Dentin Bond-Lumifor restorations to ground root dentin.

Aldehydes↗

PubMed1989

A mechanistic study of the electrochemical corrosion of the gamma 2 phase in dental amalgams. I. Evaluation of the measurements.

Conventional dental amalgams are prone to corrosion of the gamma 2 phase. In an anodic polarization experiment the presence of gamma 2 causes a current peak in the proximity of -250mV vs. SCE. Electrochemical impedance measurements carried out at this potential indicate the occurrence of an impedance curve in two parts. The high frequency part is a semi-circle with its centre below the real axis. Apart from the faradaic impedance and the double layer capacitance, the high frequency part is dominated by the behaviour of the corrosion products layer on the amalgam surface. The low frequency part has the form of a diffusion-like curve. However, since the phase angle is smaller for the above-mentioned case than for a free diffusion, this phenomenon is ascribed to diffusion in a porous layer at the interface between amalgam solution.

Corrosion↗

PubMed1989

A mechanistic study of the electrochemical corrosion of the gamma 2 phase in dental amalgams. II. Introduction of a model.

Electrochemical impedance measurements on conventional dental amalgams at an anodic polarization potential of -250 mV vs. SCE were performed. An initial evaluation was given in part I of this study; in the second part the results are interpreted in terms of a model. The latter, characterized by an equivalent circuit, is a parallel combination of a flat surface and a pore. The selection of appropriate values for the elements of the equivalent circuit leads to a better insight into the corrosion process. Although no simple relationship exists between the circuit elements and the impedance curves, an explanation was found for some typical features of these curves. As an application, the influence of the anodic polarization potential on the impedance curves was studied. Evaluation by means of the model demonstrated the importance of the characteristics of the corrosion products.

Corrosion↗

PubMed1987

Simultaneous determination of levodopa, carbidopa, 3-O-methyldopa and dopamine in plasma using high-performance liquid chromatography with electrochemical detection.

An analytical method is described which allows a fast, reliable and precise determination of levodopa and its metabolises 3-O-methyldopa and dopamine, as well as the peripheral aromatic amino acid decarboxylase inhibitor carbidopa, in a single 1 ml plasma sample of Parkinsonian patients. The compounds are quantitatively isolated on small Sephadex G-10 columns and determined by HPLC with electrochemical detection. Dihydroxybenzylamine or alpha-methyldopa are used as the internal standard. An example of therapeutic drug monitoring in a patient with fluctuations in motor performances is given. It is confirmed that interference with absorption of levodopa from the stomach by food can be partly responsible for these observed fluctuations.

Journal Article↗

PubMed1986

An application of impedance techniques to corrosion research on dental amalgam.

AC impedance techniques are finding increasing application in corrosion research. It was our aim to introduce them as a method for the evaluation of the electrochemical behaviour of dental amalgam. The complex plane plots are large depressed semicircles representing the frequency dependence of the impedance and measured at the corrosion potential. Conventional dental amalgams, in a chloride medium, exhibit a current peak in the proximity of -250 mV (vs. SCE). The corresponding impedance spectra are rather complex and difficult to interpret. The high frequency part, however, can be fitted to a semicircle with its centre lying below the real axis. An evaluation of the corresponding 'charge transfer resistance' shows that it is inversely proportional to the corrosion current density.

Corrosion↗

PubMed1985

AC impedance measurements on high copper dental amalgams.

In recent years alloys with a high copper content were developed to improve the corrosion resistance of dental amalgam by elimination of the gamma 2 (Sn-Hg) phase. The purpose of the present investigation was to compare the electrochemical behaviour of high copper amalgams obtained from single composition amalgam alloys (Indiloy; Shofu, Dental Corp., Menlo Park, USA) with those that used the additive mode of copper alloying (Dispersalloy; Johnson & Johnson, East Windsor, USA).

Copper↗

PubMed1979

A method for the measurement of dimensional change of dental amalgam.

A dimensional change occurs during the setting of a dental amalgam. A measurement circuit, which makes it possible to register these changes, is introduced. To this purpose strain gages were used. The registration instrument is tested by comparing different dental amalgam products.

Chemical Phenomena↗