Nonallelic inheritance of VH region alpha group allotypes: cell surface and serum studies in double and triple expressing rabbits.
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Animals↗
Biomedical subjects
M Mudgett
Publications and source records attributed to M Mudgett.
Isolation and characterization of distinct antibody populations from antisera directed against idiotypes of rabbit homogeneous antibodies.
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Acetylgalactosamine↗
The inheritance of antibody V regions in the rabbit: linkage of an H-chain-specific idiotype to immunoglobulin allotypes.
Anti-idiotype antibodies specific for the H chain of an homogeneous antistreptococcal antibody (4135 Ab) were prepared by injection of recombinant molecules consisting of the H chains from 4135 Ab and L chains isolated from the injected rabbit. The antibodies prepared in this fashion (anti-HId) were specific for the VH region of 4135 Ab. Using this preparation in an inhibition of binding assay, sera from rabbits related and unrelated to 4135 were screened for the presence of the 4135 HId. It was found that about 45% of the related rabbits, when immunized with streptococcal Group C vaccine, produced antibodies with a cross-reactive idiotype, while less than 10% of similarly immunized unrelated rabbits produced molecules bearing HId. The expression of HId was linked to the a3 allotype present in the H chain allogroup J. Antibody molecules bearing the HId determinant were isolated from heterozygous (a1a3 and a2a3) rabbits and shown to express the a3 allotype. One rabbit lacking the a3 allotype produced significant amounts of antibodies expressing HId. These antibodies were found to express both VH and CH allo-types characteristic of the J allogroup, although neither allotype was found in a preimmune IgG sample from this rabbit.
Animals↗
Peptide sequencing: the utility of chemical ionization mass spectrometry.
Chemical ionization mass spectrometry is used at low resolution to determine the sequences of permethylated acetyl peptides. The method has been tested with 45 peptides, between 2 and 5 residues long, including examples of all of the common amino acids except cysteine and N-terminal asparagine. The isobutane chemical ionization spectra contain three principal types of N-terminal sequence ion and one type of C-terminal sequence ion. The redundant information available from these four types of sequence ion increase the reliability of the sequence determination. In prospect, isobutane chemical ionization mass spectrometry seems to be a useful technique for peptide sequence determination, and may have advantages in some cases.
Amino Acid Sequence↗
Nonallelic behavior of rabbit variable-region allotypes.
Group a allotypes not detected by qualitative typing or anticipated from breeding data (latent allotypes) were detected at low levels in 50% of normal rabbit sera tested. The latent allotypes, which were serologically identical to allotypes of pooled IgG, were detected in sera from rabbits with all possible combinations of group a allotypes and their occurrence in individual rabbits was transitory and sporadic. These findings give reason to question the assumption that the synthesis of immunoglobulin allotypes is directed by allelic structural genes.
Animals↗